اثرات افزودن سطوح مختلف سلنیوم آلی بر پاسخ سیستم ایمنی و بیان ژن های اینترفرون گاما، اینترلوکین 2 و 6 در موش‌های تحت تنش گرمایی
محورهای موضوعی : زیست شناسی
1 - گروه علوم دامی واحد علوم و تحقیقات
کلید واژه: سلنیوم آلی, اینترفرون گاما, اینترلوکین2, اینترلوکین6, پاسخ سیستم ایمنی, موش,
چکیده مقاله :
دمای بالای محیط سبب ایجاد تنش اکسیداتیو در بدن حیوانات می شود. سلنیوم ماده معدنی کمیابی است که در شرایط تنش بر سلامتی و عملکرد بدن اثر دارد. مطالعات محدودی در زمینه اثر دوزهای مختلف سلنیوم در شرایط تنش گرمایی طولانی مدت بر پاسخ سیستم ایمنی و بیان ژن‌های مرتبط با التهاب وجود دارد. پژوهش کنونی به منظور تعیین اثرات دوزهای مختلف سلنیوم از مکمل سلنیوم-متیونین بر پاسخ سیستم ایمنی و بیان ژن‌های اینترفرون گاما، اینترلوکین 2 و 6 انجام شد. 25 سر موش صحرایی ماده به طور تصادفی به پنج گروه و هر گروه پنج سر موش تقسیم شدند. تعداد 5 سر موش در کل دوره آزمایش در دمای استاندارد و بقیه موش ها در تنش گرمایی قرار داده شدند. موش های گروه کنترل منفی (بدون تنش گرمایی) و کنترل مثبت (تنش گرمایی) پلت استاندارد بدون افزودنی و سه گروه دیگر به ترتیب پلت استاندارد به اضافه 0/15، 0/30 و 0/45 میلی گرم در کیلوگرم سلنیوم از مکمل سلنیوم-متیونین به مدت 30 روز دریافت کردند. افزودن مکمل سلنیوم به جیره سبب افزایش بیان ژن اینترفرون گاما در موش‌های تحت تنش گرمایی شد. بیان نسبی ژن اینترلوکین2 در گروه‌های دریافت کننده مکمل سلنیوم نسبت به گروه شاهد مثبت پایین‌تر بود. همچنین افزودن مکمل سلنیوم به جیره سبب کاهش بیان ژن اینترلوکین6 در مقایسه با تیمار شاهد مثبت گردید، به طوری که پایین‌ترین بیان ژن اینترلوکین6 در موش‌های تحت تنش گرمایی مربوط به تیمار0/45 میلی‌گرم بود. نتایج حاکی از افزایش غلظت ایمونوگلوبولین G با افزودن مکمل سلنیوم به جیره بود.
High ambient temperature causes oxidative stress in animals. Selenium is a trace mineral that has an effect on the health and performance of the body in stressful conditions There are limited studies on the effect of different doses of selenium in the conditions of long-term heat stress on immune system response and the expression of genes related to inflammation. The present study was conducted to determine the effects of different doses of selenium from selenium-methionine supplement on immune system response and the expression of interferon gamma, interleukin-2 and 6 genes in rats during the hot season.25 female rats were randomly divided into five groups and five replicates. Five rats were placed in standard temperature during the experiment period and the rest of the rats were placed in heat stress. Rats in the negative control group (without heat stress) and positive control (heat stress) fed with standard pellet without additive and the other three groups fed with standard pellet plus 0.15, 0.30 and 0.45 mg selenium/kg as selenium-methionine supplement for 30 days. Adding selenium supplement to the diet increased the expression of interferon gamma gene in rats under heat stress. The relative expression of the interleukin-2 gene was lower in the groups receiving selenium supplements than in the positive control group. Also, adding selenium supplement to the diet decreased the expression of interleukin-6 gene compared to the positive control treatment. The results indicated an increase in the concentration of immunoglobulin G with the addition of selenium to the diet.
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