بررسی تأثیر مکمل روی بر سطح سرمی روی، ویتامین A و بتاکاروتن در گاو شیری
محورهای موضوعی : پژوهش های بالینی دام های بزرگ
کلید واژه: روی, بتاکاروتن, گاو شیری, ویتامین A, سرم,
چکیده مقاله :
این مطالعه به منظور ارزیابی نقش مکمل سولفات روی بر سطح سرمی ویتامین A و بتاکاروتن در گاوهای شیری انجام گرفت. 17 رأس گاو شیری هم سن با شرایط تغذیه‌ای، مدیریتی و تولید تقریباً یکسان در دو گروه شاهد (7 رأس) و تیمار (10 رأس) قرار گرفتند. در گروه تیمار علاوه بر غذای معمول، روزانه به میزان mg500 مکمل سولفات روی در جیره غذایی به مدت 45 روز اضافه شد. در روزهای صفر (قبل از شروع مصرف مکمل)، 15، 30 و 45 از گاوهای هر دو گروه از ورید دم خونگیری به عمل آمد و میزان سرمی روی با کیت بیوشیمیایی و مقادیر سرمی ویتامین A و بتا کاروتن با روش اسپکتروفتومتری با استفاده از محلول -N هگزان اندازه‌گیری شد. میانگین سطح سرمی روی در گروه تیمار افزایش معنی‌داری را در مقایسه با گروه شاهد در روزهای 15، 30 و 45 نشان داد (000/0= P). میانگین سطح سرمی ویتامین A در گره تیمار در روز 15 افزایش معنی‌داری نداشت، ولی در روزهای 30 و 45 افزایش معنی‌دار بود (به ترتیب 016/0= p و 004/0= P). بتاکاروتن سرم نیز از روز 15 به بعد در گروه تیمار افزایش نشان داد ولی در هیچ کدام از زمانها افزایش معنی‌دار نبود. در گروه تیمار همبستگی بین سطح سرمی ویتامین A با میزان سرمی روی فقط در روز 45 معنی‌دار بود (716/0= r و 05/0< p) و در بقیه زمانها معنی‌دار نبود ولی در مورد بتاکاروتن ارتباط معنی‌دار با روی سرم وجود نداشت. نتیجه نهایی اینکه مکمل سولفات روی باعث افزایش سطح سرمی روی و ویتامین A در گاوهای شیری می‌شود.
This study has been done in order to assess the supplementation of zinc sulfate on serum level of vitamin A and Beta carotene in dairy cows. 17 heads of milky cows with equal conditions of feed management and production and coeval were located in two control group (7 heads) treatment group (10 heads). In treatment group in addition to normal food, 500 mg supplement zinc sulfate has added to diet for 45 days. Blood samples were taken in days zero (before of initial administration supplement), 15, 30 and 45 from cows in both groups of tail vein. Average zinc serum by biochemical kit and serum quantity of vitamin A and Beta- carotene by spectrophatometry and using N. Hexane solution were measured. The average of serum level of supplement group in comparision to control group in 15, 30, 45 days, shows meaningful increase (p=0/000). Average serum level of vitamin A in supplement group in 15 days didn't has significant increase, but in 30 and 45 days had significant increase (p=0/016 , p=0/004, respectively). Serum Betacarotene in creased after day 15 in supplement group but any time that was not meaning increase but in 30 and 45 days had meaningful increase. (P=0.016, P=0.004). Serum Betacarotene incrased after day 15 in care group but any time, that was not meaningful increase. In supplement group the correlation between serum level of vitamin A with serum level of zinc was meaningful only day 45. (r= 0.716, p<0.05), In other times it was not meaningful, but in the case of Betacarotene, There wasn't meaningful relation with zinc serum. The result is that, zinc sulfate complementary increases serum level of zinc and vitamin A in milky cows and can be useful.
1- Abdulkareem, T. and Al-Haboby, A.(2003): Sperm abnormalities associated with vitamin deficiency in rams.- Small animal research. Elseviere Inc. Vol 57 , Issue 1. pp 67-71
2- Abdullah Eryavuz and Burk, Dehority, A.(2009): Effects of supplemental zinc concentration on cellulose digestion and cellulolytic and total bacterial numbers in vitro, Animal Feed Science and Technology, Volume 151, Issues 3-4, PP: 175-183.
3- Al-Sultan, S.I.(2008): Immuno- stimulant effects of ascorbic acid in colostrums-deprived neonatal camels. Department of public health and animal husbandry.college of veterinary medicine and animal resources, King faisal university.
4- Asadian, A. and Mezez, M.(1996): Effects of vitamins A and E supplementation on Vitamins A and E Status of blood Plasma , liver and tail fat- tailed sheep , Small Ruminant research, Volume 23 , Issue 1, PP: 1-6.
5- Azizzadeh, M., Mohri, M. and Seifi, H.A.(2005): Effect of oral zinc supplementation on hematology, serum biochemistry, performance, and health in neonatal dairy calves. Comparative Clinical Pathology, 14: 67–71
6- Chesters, J.K.(1983): Zinc metabolism in animals: pathology, immunology and genetics. Journal of inherited Metabolic Disease, 6 (1): 34-38
7- Cope, C.M., Mackenzie, A., Wilde and, D. Sinclair, L.A.(2009): Effects of level and form of dietary zinc on dairy cow performance and health, J. Dairy Sci. 92:2128-2135.
8- Cummins, K.A. and Brunner, C.J. (1991): Effect of calf Housing on Plasma Ascorbate and Endocrine and Immune function . J. Dairy Sci., 74: 1582-1588
9- Dubick, M.A.,Williams, C., Elgjo, G.I., Kramer, G.C.(2005): High dose vitamin c infusion reduce fluid requirements in the resuscitation of burn-injured sheep.Pub Med - U.S.national library of medicine and the National institute of health.Vol 24.PP: 139-144
10- Erel, O., Mehmet, S., Gurel. (1998): Alterations of serum selenium, zinc, copper, and iron concentrations and some related antioxidant enzyme activities in patients with cutaneous leishmaniasis. Bological Trace Element Reaserch .Vol 65, Number 3, Pges 271-281
11- Golalipour, M., Mansourian, A. and Keshtkar, A.(2006): Serum Zinc Levels in Newborns with Neural Tube Defects.Indian pediatrics 812.Volume 43-september 2006
12- Harwey, L., Foxall, R., McAnena, L. and Susan, J.(2007): Effect of high-dose iron supplements on fractional zinc absorption and status in pregnant women. American Journal of Clinical Nutrition, Vol. 85, No. 1, 131-136, January 2007
13- Howard, J. L. and Smith, R.A. (1999): Current veterinary therapy, food animal practice. 4th edition, W.B. Saunders company, pp: 181, 186-187, 706
14- Irvin, V.C.R.(1974): Influence of disease in colostrum deprived calves. Vet. Rec., 94: 105.
15- Kincaid, R.L., Chew, B.P. and Cronrath, J.D.(1997): Zinc oxide and amino acids as sources of dietary zinc for calves: effects on uptake and immunity. J. Dairy Sci. 80:1381-1388.
16- Kolb, E.(1992): Neure Erkenntnisse Zur Bedeutung der Askorbinsaure fur Haustiere and Zu ihrer Anwendung in der Veterinarmedizin, Tierarztl. Umschau, 47:163-175.
17- Kumar, S., Awashti, S.(2004): Blood Zinc Levels in Children Hospitalized with Severe Pneumonia:A case control study. Indian pediatrics. Volume 41.pages 486-491
18- Nocek, J. E., Socha, M. T. and Tomlinson, D.J.(2006): The effect of trace mineral fortification level and source on performance of dairy cattle. J. Dairy Sci. 89:2679-2693.
19- Radostits, O. M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D.(2007): Veterinary Medicine, a textbook of the diseases of cattle, horses, sheep, pigs and goats, 10th edition. Elsevier publishing, pp:508-516, 1526-1555, 1731-1732
20- Suzki, J. and Katch, N., 1990, A simple and cheep methods for measuring serum vitamin A in cattle using only a spectrophotometer, Jpn. J. Vet. Sci., 52: 1281- 1283.
21- Tomlinson, D.J., Socha, M.T. and DeFrain, J.M.(2008): Role of trace minerals in the immune system. Page 39-52. In: Proc. Penn State Dairy Cattle Nutrition Workshop. Grantville, PA.
22- Wright, C.L., Spears, J.W. and Webb, K.E.(2008): Uptake of zinc from zinc sulfate and zinc proteinate by ovine ruminal and omasal epithelia. J. Anim. Sci. 86:1357-1363.
_||_