بررسی اثر استفاده از سطوح مختلف کنسانتره در جیره غذایی بر میزان سنتز پروتئین میکروبی در شکمبة گاومیش بومی استان مازندران
محورهای موضوعی : پژوهش های بالینی دام های بزرگ
کلید واژه: گاومیش, جیره غذایی, مشتقات پورینی, سنتز پروتئین میکروبی,
چکیده مقاله :
عوامل مختلفی از جمله فراهم بودن منبع انرژی، تامین نیتروژن، pH شکمبه، نرخ عبور از شکمبه، حذف تک‌یاخته‌ها از شکمبه و مواد معدنی بر میزان سنتز پروتئین میکروبی اثر می‌گذارند. به طور طبیعی تامین انرژی، اولین عامل محدود کننده در سنتز پروتئین میکروبی در شکمبه به شمار می‌رود. هدف از این تحقیق بررسی میزان تأثیر سطوح مختلف کنسانتره بر مقدار سنتز پروتئین میکروبی با استفاده از روش دفع مشتقات پورینی در ادرار می باشد. اثر 4 جیره غذایی شامل: جیره تمام علوفه‌ای، جیرة حاوی 15٪ ، 30٪ و 45٪ کنسانتره در قالب طرح مربع لاتین روی 4 رأس گاومیش نر بومی مازندران با متوسط وزنی 10±140 کیلوگرم انجام گرفت. میزان مشتقات پورینی دفع شده برای جیره‌های تمام علوفه‌ای، حاوی 15% ، 30 % و 45% کنسانتره به ترتیب 54/14، 11/18، 59/21، 55/24 (میلی‌مول در روز) و میزان نیتروژن میکروبی تولید شده برای جیره‌های فوق به ترتیب 76/38، 44/60، 48/81، 43/99 (گرم در روز) بود. میزان پروتئین میکروبی سنتز شده نیز برای این جیره‌ها به ترتیب 28/242، 74/377، 23/509 و 42/621 گرم در روز بود. با افزایش سطح کنسانتره در جیرة غذایی، میزان مشتقات پورینی دفع شده در ادرار و نیتروژن یا پروتئین میکروبی سنتز شده در شکمبه به طور معنی‌داری افزایش یافت (01/0>p).
Several factors such as source of energy, nitrogen supply, rumen pH, passage rate, defaunating the rumen and mineral supply affect on microbial protein synthesis in rumen. Energy is the first limiting factor in microbial protein supply in the rumen. This study aims at measuring the microbial protein synthesis in Iranian buffalo's rumen. Simultaneously, it tries to study the various effects of concentration on the amount of microbial protein synthesis. Four Iranian male swamp buffalo with the average live body weight of 140±10 kg were used for this study. It has been experimented four diets consist of 0 % (all forage diet), 15%, 30% and 45% concentrate in a Latin square design. The results of this experiment indicated that increasing concentrate level in diet of swamp buffaloes affected the amount of PD excreted in urine. The amount of total purines derivatives excreted in urine were 14.54, 18.11, 21.59 and 24.55 mmol/day, microbial nitrogen synthesized were 38.76, 60.44, 81.48 and 99.43 g/day and microbial protein supply were 242.28, 377.74, 509.23 and 621.42 g/day respectively. The results of this experiment indicated that increasing the concentrate level in diet of swamp buffaloes grew the amount of microbial protein synthesized in rumen.
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