ارزیابی اثر ضد قارچی انار (Punica granitum L.) رقم ملس ساوه بر قارچ عامل پوسیدگی میوه انار آلوده به کرم گلوگاه
محورهای موضوعی : زیست شناسی
عذرا عطایی عظیمی
1
(دانشیار، گروه زیست‌شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران )
بابک دلنواز هاشملویان
2
(دانشیار، گروه زیست‌شناسی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران)
رضا رضاخانلو
3
(استادیار، گروه کشاورزی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران)
کلید واژه: انار رقم ملس ساوه, فنل, آلکالوئید, آسپرژیلوس, پریکارپ, قارچ, کرم گلوگاه, پوسیدگی میوه.,
چکیده مقاله :
هدف: هدف پژوهش حاضر پاسخ به این سوال است که کدام ترکیب میوه انار (L. Punica granitum) رقم ملس ساوه باعث رشد یا جلوگیری از رشد قارچ عامل پوسیدگی میوه آلوده به کرم گلوگاه می‌شود.
مواد و روش‌ها: در این پژوهش، اجزای مختلف انار شامل پوست میوه، دانه، آب انار و رب انار رقم ملس ساوه تهیه شد. محتوای فنلی و آلکالوئیدی کل بخش‌ها با استفاده از حلال‌‌هایی مثل آب، الکل و استن، استخراج و اندازه‌‌گیری گردید. قارچ آسپرژیلوس (Asperjillus Sp..) از محل پوسیدگی آلوده به کرم گلوگاه جدا و شناسایی شد. اثر عصارههای آبی و الکلی اجزای میوه انار روی این قارچ مورد مطالعه قرار گرفت.
یافته‌ها: نتایج نشان داد که آب و رب خشک انار، بیشترین آلکالوئید و فنل را دارند. اثر عصارههای آبی و الکلی روی قارچ آسپرژیلوس نشان داد عصارههای آبی و الکلی مانع رشد این قارچ می‌‌شوند، ولی اثر بازدارنده الکلی پریکارپ از همه بیشتر است.
نتیجه‌گیری: اجزای میوه انار ملس ساوه سرشار از ترکیبات آلکالوئید و فنلی هستند. اثر ضدقارچی عصارههای آبی و الکلی پریکارپ میوه می‌تواند از رشد قارچ جلوگیری کند
Purpose: The purpose of this research is to answer the question, which composition of pomegranate fruit (L. Punica granitum) of the variety Meles Saveh causes the growth or prevents the growth of the fungus that causes fruit rot infected with neck worm (Ectomyelois ceratoniae)
Materials and methods: In this research, different parts of pomegranate including fruit skin, seeds, pomegranate juice and pomegranate paste of Meles Saveh variety were prepared. The phenolic and alkaloid content of all parts were extracted and measured using solvents such as water, alcohol, and acetone. Aspergillus fungus (Asperjillus Sp.) was isolated and identified from the decay site infected with neck worm. The effect of aqueous and alcoholic extracts of pomegranate fruit components on this fungus was studied.
Findings: The results showed that pomegranate juice and dry paste have the most alkaloids and phenols. The effect of aqueous and alcoholic extracts on Aspergillus mushroom showed that aqueous and alcoholic extracts prevent the growth of this fungus, but the inhibitory effect of alcoholic pericarp is the most.
Conclusion: Meles Saveh pomegranate fruit components are rich in alkaloid and phenolic compounds. Antifungal effect of aqueous and alcoholic extracts of fruit pericarp can prevent the growth of fungus.
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