ارزیابی تنوع ژنتیکی گیاه بارهنگPlantago major L. با نشانگر مولکولی ISSR در منطقه بروجرد
محورهای موضوعی :
گیاهی
نرجس آزادبر
1
,
زهرا سادات موسوی خوانساری
2
,
محمدمهدی دهشیری
3
,
رضا یاری
4
1 - کارشناسی ارشد، گروه زیست‌شناسی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران.
2 - کارشناسی ارشد، گروه زیست‌شناسی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
3 - دانشیار، گروه زیست‌شناسی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
4 - استادیار، گروه زیست‌شناسی، مرکز تحقیقات گیاهان دارویی، سلامت و امنیت غذایی؛ واحد بروجرد، دانشگاه آزاد اسلامی،
بروجرد، ایران
تاریخ دریافت : 1400/04/24
تاریخ پذیرش : 1400/07/10
تاریخ انتشار : 1401/01/01
کلید واژه:
Plantago major L,
تنوع ژنتیکی,
گیاه بارهنگ,
ISSR,
UPGMA,
بروجرد,
چکیده مقاله :
هدف: منطقه بروجرد در استان لرستان یکی از بهترین مناطق کم‌تر شناخته‌شده رویشگاه‌های طبیعی بارهنگ است. هدف مطالعه حاضر شناسایی رویشگاه‌های طبیعی آن در منطقه بروجرد و ارزیابی تنوع ژنتیکی در اکوتیپ‌ها با مارکر مولکولی ISSR است.
مواد و روش‌ها: در این پژوهش تنوع ژنتیکی 23 نمونه از 6 جمعیت بارهنگ (L. major Plantago) مورد مطالعه قرار گرفت. DNA استخراج شده هر 23 نمونه، با روش کیت انجام پذیرفت. جهت واکنش PCR از 10 پرایمر با کدهای بین‌المللی استفاده شد. حضور یا عدم حضور باند به‌صورت ماتریس یک/صفر در برنامه SPSS ذخیره شد.
یافته‌ها: از کل 1632 باند تولیدشده، 200 باند (آلل) پلیمرف بودند که معادل 24% پلیمرفیسم محاسبه شد. طول قطعات ایجادشده بین bp 300 تا 1650 متغیر بود. پرایمر P8 بیشترین باند مشترک به تعداد 61 باند و پرایمر P9 کم‌ترین باند مشترک به تعداد 7 باند را در جمعیت‌ها تولید کرده‌اند. بیشترین تعداد باند تکثیرشده 206 باند مربوط به آغازگر P8 و کم‌ترین تعداد باند تولیدشده 125 باند مربوط به آغازگر P5 بود.
نتیجه‌گیری: نتایج نشان می‌دهد که مارکرهای ژنتیکی ISSR بویژه پرایمر P8 به خوبی می‌تواند در مطالعات فیلوژنتیکی گیاه بارهنگ بکار رود. منطقه بروجرد با وجود وسعت کم، جهت رویش اکوتیپ‌های متنوع گیاه بارهنگ مناسب است؛ لذا حفظ و نگهداری این رویشگاه طبیعی و نیز تکثیر اکوتیپ‌های مختلف باید مورد توجه قرار گیرد.
چکیده انگلیسی:
Objective: Borujerd region in Lorestan province is one of the best and less known natural habitats of plantago major L. The aim of the current study is to identify its natural habitats in Borujerd region and to evaluate genetic diversity in ecotypes with ISSR molecular markers.
Materials and methods: In this research, the genetic diversity of 23 samples from 6 populations of Plantago major L. was studied. The extracted DNA of all 23 samples was done by the kit method. 10 primers with international codes were used for PCR reaction. The presence or absence of the band was stored as a one/zero matrix in the SPSS program.
Findings: Out of the total 1632 bands produced, 200 bands (alleles) were polymorphism, which was calculated as 24% of polymorphism. The length of the generated fragments varied between 300 and 1650 bp. Primer P8 produced the most common bands with 61 bands and primer P9 produced the least common bands with 7 bands in the populations. The highest number of amplified bands was 206 bands related to P8 primer and the lowest number of produced bands was 125 bands related to P5 primer.
Conclusion: The results show that the ISSR genetic markers, especially the P8 primer, can be used in phylogenetic studies of leek plant. Despite its small area, Borujerd region is suitable for the growth of various ecotypes of the Plantago major L, and therefore the preservation and maintenance of this natural habitat and the propagation of different ecotypes should be considered.
منابع و مأخذ:
Ghasemi F, Jalili A, Mirzadeh Vaghefi S & Kazemi S. Cytogenetic study of Plantago major populations in North West of Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 2016; 24(2): 305-314. (In persian)
Najafian Y, Hamedi SS, Farshchi MK & Feyzabadi Z. Plantago major in Traditional Persian Medicine and modern phytotherapy: a narrative review. Electron Physician. 2018; 10(2): 6390-6399.
Ramezani M & Rahimi M. Study of phylogenetic relationships and genetic diversity of Plantago ovata ecotypes using morpho-phenological traits and ISSR markers. Plant Genetic Resources. 2017; 4(1): 63-74. (In persian)
Keivani M, Mehregan I & Albach D. Genetic diversity and population structure of Plantago major (Plantaginaceae) in Iran. 2020; 26: 111-124.
Nejati V & khaneshi F. Effect of hydroalcoholic extract of Plantago major leaf on the testis morphology, sperm parameters and testosterone level in streptozotocin-induced diabetic rats. The Horizon of Medical Sciences. 2014; 20(1): 49-55. (In persian)
Jafari R & Kamali K. Faunestic study of ladybird (Col.: Coccinellidae) in Lorestan rrovince and report of new records in Iran. New Findings in Agriculture. 2007; 1(4): 349-359.
(In persian)
Hashemi M, Nabipour F, Yari R & Jafari R. The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of Hippodamia variegate (Col.:Coccinellidae) in Lorestan provinces, Iran. Journal of Entomological Research. 2018; 9(4): 393-400. (In persian)
Hassani M‚ Babaii A & Moslemkhani K. Study of Chelidonium majus genetic diversity by ISSR markers in Iran. New Cellular and Molecular Biotechnology Journal. 2016; 6(22): 91-96. (In persian)
Shazdehahmadi M & Kharrazi M. Application of ISSR molecular markers for genetic diversity study of some Tobacco genotypes. Plant Genetic Resources. 2016; 2(2): 33-46. (In persian)
Wongsawad P & Peerapornpisal Y. Molecular identification and phylogenetic relationship of green Algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences. 2014; 21: 505-510.
Kaswan V, Joshi A & Maloo AR. Assessment of genetic diversity in Isabgol (Plantago ovata) using Random Amplified Polymorphic DNA (RAPD) and Inter-simple Sequence Repeat (ISSR) markers for developing crop improvement strategies. African Journal of Biotechnology. 2013; 12(23): 3622-3635.
Mohammadi S, Mehrabi AA, Arminian A & Fazeli A. Genetic diversity structure of Aegilops cylindrica accessions revealed by genomic ISSR markers. Plant Genetic Resources. 2014; 1(1): 13-26. (In persian)
Gheitarani B‚ Erfani-Moghadam J & Fazeli A. Evaluation of genetic diversity among some common Fig using RAPD and ISSR molecular markers. Plant Genetic Resources. 2019; 6(2): 43-54. (In persian)
Amiri P, Ismaili A & Hadian J. Evaluation of genetic diversity of Styrian pumpkin (Cucurbita pepo styriaca) populations, using ISSR molecular markers. Plant Genetic Resources. 2018; 4(2): 17-28. (In persian)
Salami A, Pahlevani M, Zenalinezhad K & Esmaeilzadeh Moghaddam M. Genetic variation pattern of Iranian Wheat landraces Based on ISSR molecular markers and morphological traits. Plant Genetic Resources. 2018; 5(1): 87-100. (In persian)
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Ghasemi F, Jalili A, Mirzadeh Vaghefi S & Kazemi S. Cytogenetic study of Plantago major populations in North West of Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 2016; 24(2): 305-314. (In persian)
Najafian Y, Hamedi SS, Farshchi MK & Feyzabadi Z. Plantago major in Traditional Persian Medicine and modern phytotherapy: a narrative review. Electron Physician. 2018; 10(2): 6390-6399.
Ramezani M & Rahimi M. Study of phylogenetic relationships and genetic diversity of Plantago ovata ecotypes using morpho-phenological traits and ISSR markers. Plant Genetic Resources. 2017; 4(1): 63-74. (In persian)
Keivani M, Mehregan I & Albach D. Genetic diversity and population structure of Plantago major (Plantaginaceae) in Iran. 2020; 26: 111-124.
Nejati V & khaneshi F. Effect of hydroalcoholic extract of Plantago major leaf on the testis morphology, sperm parameters and testosterone level in streptozotocin-induced diabetic rats. The Horizon of Medical Sciences. 2014; 20(1): 49-55. (In persian)
Jafari R & Kamali K. Faunestic study of ladybird (Col.: Coccinellidae) in Lorestan rrovince and report of new records in Iran. New Findings in Agriculture. 2007; 1(4): 349-359.
(In persian)
Hashemi M, Nabipour F, Yari R & Jafari R. The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of Hippodamia variegate (Col.:Coccinellidae) in Lorestan provinces, Iran. Journal of Entomological Research. 2018; 9(4): 393-400. (In persian)
Hassani M‚ Babaii A & Moslemkhani K. Study of Chelidonium majus genetic diversity by ISSR markers in Iran. New Cellular and Molecular Biotechnology Journal. 2016; 6(22): 91-96. (In persian)
Shazdehahmadi M & Kharrazi M. Application of ISSR molecular markers for genetic diversity study of some Tobacco genotypes. Plant Genetic Resources. 2016; 2(2): 33-46. (In persian)
Wongsawad P & Peerapornpisal Y. Molecular identification and phylogenetic relationship of green Algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences. 2014; 21: 505-510.
Kaswan V, Joshi A & Maloo AR. Assessment of genetic diversity in Isabgol (Plantago ovata) using Random Amplified Polymorphic DNA (RAPD) and Inter-simple Sequence Repeat (ISSR) markers for developing crop improvement strategies. African Journal of Biotechnology. 2013; 12(23): 3622-3635.
Mohammadi S, Mehrabi AA, Arminian A & Fazeli A. Genetic diversity structure of Aegilops cylindrica accessions revealed by genomic ISSR markers. Plant Genetic Resources. 2014; 1(1): 13-26. (In persian)
Gheitarani B‚ Erfani-Moghadam J & Fazeli A. Evaluation of genetic diversity among some common Fig using RAPD and ISSR molecular markers. Plant Genetic Resources. 2019; 6(2): 43-54. (In persian)
Amiri P, Ismaili A & Hadian J. Evaluation of genetic diversity of Styrian pumpkin (Cucurbita pepo styriaca) populations, using ISSR molecular markers. Plant Genetic Resources. 2018; 4(2): 17-28. (In persian)
Salami A, Pahlevani M, Zenalinezhad K & Esmaeilzadeh Moghaddam M. Genetic variation pattern of Iranian Wheat landraces Based on ISSR molecular markers and morphological traits. Plant Genetic Resources. 2018; 5(1): 87-100. (In persian)
