تاثیر غلظت‌های مختلف نیترات پتاسیم بر خصوصیات فیزیولوژیکی و بیوشیمیایی ذرت (Zea mays L.) تحت شرایط تنش شوری
محورهای موضوعی : فیزیولوژی و متابولیسم بذرهما زارعی 1 , محمد صدقی 2 , سلیم فرزانه 3 , هانیه سعادت 4
1 - دانش آموخته کارشناسی‌ارشد علوم و تکنولوژی بذر دانشگاه محقق اردبیلی، اردبیل، ایران
2 - استاد گروه علوم و مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
3 - دانشیار گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران
4 - دانش آموخته دکتری اکولوژی گیاهان زراعی دانشگاه محقق اردبیلی، اردبیل، ایران
کلید واژه: آنزیم‌های آنتی‌اکسیدانت, پرایمینگ, کلرید سدیم, نیترات پتاسیم,
چکیده مقاله :
به‌منظور بررسی اثر پرایمینگ با غلظت‌های مختلف نیترات پتاسیم بر روی خصوصیات فیزیولوژیکی و بیوشیمیایی ذرت تحت تنش شوری، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا گردید. تیمارها شامل سه سطح مختلف شوری با غلظت‌های صفر، 100 و ۲۰۰ میلی‌مولار و سطوح مختلف محلول نیترات پتاسیم با غلظت‌های صفر، 5/1 و ۳ درصد بود. نتایج نشان داد که بیشترین درصد جوانه‌زنی، سرعت جوانه‌زنی، یکنواختی جوانه‌زنی، طول ریشه‌چه و ساقه‌چه، وزن تر و خشک ریشه‌چه و ساقه‌چه در پرایمینگ با نیترات پتاسیم 3 درصد و بدون شوری حاصل شد. میانگین مدت جوانه‌زنی 53 درصد نسبت به شاهد کاهش نشان داد. فعالیت آنزیم‌های کاتالاز، پراکسیداز و سوپراکسیددیسمیوتاز در پرایمینگ با نیترات پتاسیم 3 درصد و شوری 200 میلی‌مولار نسبت به شاهد به‌ترتیب 56، 68 و 67 درصد افزایش نشان دادند. شوری موجب کاهش فعالیت آنزیم آمیلاز شد، ولی پرایمینگ با نیترات پتاسیم فعالیت این آنزیم را افزایش داد. محتوای کل پروتئین بذر در پیش تیمار نیترات پتاسیم 3 درصد و بدون شوری حدود 63 درصد افزایش داشت. به‌طور کلی، پرایمینگ با محلول نیترات پتاسیم 3 درصد را می‌توان به‌عنوان مناسب‌ترین تیمار برای بهبود خصوصیات فیزیولوژیکی و بیوشیمیایی ذرت تحت تنش شوری در نظر گرفت.
In order to investigate the effect of different concentrations of KNO3 on physiological and biochemical properties of maize (Zea mays L.) under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2021. Treatments included three salinity levels (0, 100 and 200 Mm) and three KNO3 levels (0, 1.5 and 3%). The results showed that the highest germination percentage (GP), germination rate (GR), germination uniformity (GU), radicle and plumule length (RL and PL) and radicle fresh and dry weight (RFW and RDW) was related to priming with KNO3 3% and without salinity. Mean of germination time (MGT) compared to the control showed a reduction about 53%. The activity of catalase, peroxidase and superoxide dismutase enzymes in priming with KNO3 3% and 200 mM salinity compared to the control showed an increase about 56, 68 and 67%, respectively. Salinity decreased the activity of amylase, but priming with KNO3 increased the activity of this enzyme. The total seed protein content in KNO3 3% pretreatment and without salinity was increased about 63%. In general, priming with KNO3 3% solution can be considered as the best treatment to improve the physiological and biochemical properties of corn under salinity stress.
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