تأثیر دو محیط آب – خزه و خاک پیت‌ماس بر تعداد کوکون‌گذاری، وزن کوکون و تعداد تلفات زالوی شرقی (Hirudo orientalis)
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
حمیدرضا بیدمال
1
(دانشکده شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان)
محمد سوداگر
2
(دانشیار گروه تکثیر و پرورش آبزیان، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.)
کلید واژه: کوکون‌گذاری, آب-خزه, خاک پیت‌ماس, تلفات, زالوی شرقی,
چکیده مقاله :
زمینه و هدف: کلرید کادمیوم باعث ایجاد صدمه کبدی می گردد. در این مطالعه اثر حفاظتی عصاره هیدروالکلی گل ساعتی بر علیه سوء عملکرد کبدی القاء شده توسط کلرید کادمیوم در موش های صحرایی نر بالغ مورد بررسی قرارگرفت. روش کار: 54 سر موش صحرایی نربالغ نژاد ویستار به6 گروه 9 تایی تقسیم شدند. گروه کنترل،گروه شاهد1: 0.2ml/kg آب مقطر روزانه به صورت درون صفاقی به عنوان حلال دریافت کردند.گروه شاهد2: 2mg/kg کلرید کادمیوم روزانه به صورت درون صفاقی به مدت 21 روز دریافت کردند.گروه های تجربی3و2و1: 2mg/kg کلرید کادمیوم روزانه به صورت درون صفاقی به مدت 21 روز و سپس 150,300,450mg/kg عصاره هیدروالکلی بخش های هوایی گل ساعتی روزانه به صورت درون صفاقی به مدت30روز دریافت کردند. سطوح سرمی ALT,AST,ALP,GGT,LDH،آلبومین، بیلی روبین و پروتئین توتال اندازه گیری شدند. آزمایشات آسیب شناسی بافتی کبد بعد از رنگ آمیزی هماتوکسیلن وائوزین انجام شد. یافته‌ها: میانگین غلظت سرم ALT، ALp < /strong>،LDH ، GGT و بیلی روبین در تمام گروه های تجربی دریافت کننده کلرید کادمیوم و عصاره هیدروالکلی بخش های هوایی گل ساعتی و کلرید کادمیوم نسبت به گروه دریافت کننده کلرید کادمیوم کاهش معنی داری نشان داد. میانگین غلظت سرم AST در گروه های تجربی دریافت کننده 3و2 نسبت به گروه دریافت کننده کلرید کادمیوم کاهش معنی داری نشان داد. میانگین غلظت پروتئین توتال و آلبومین سرم در گروه های تجربی 3و2 نسبت به گروه دریافت کننده کلرید کادمیوم افزایش معنی داری نشان داد(05/0>p < /strong>). نتیجه‌گیری: نتایج این مطالعه نشان داد که عصاره هیدروالکلی بخش های هوایی گل ساعتی دارای اثر حفاظتی بر سوء عملکردکبدی القاء شده توسط کلرید کادمیوم در موش های صحرایی نر بالغ می باشد.
Background: In recent years, leeches have been used to treat some diseases, extract many enzymes and substances that are effective in treating diseases.This study aims to influence the two environments of water-moss and peat moss soil on the number of cocoons, the weight of the cocoons and the losses in the oriental leech. Method: For this purpose, 300 productive leeches for 45 days in 6 treatments and 3 repetitions per treatment: treatment (1) 10 leeches in water-moss, treatment (2) 15 leeches, treatment (3) 25 pieces Leeches, treatment (4) 10 pieces of leeches in Peat mosss, treatment (5) 15 pieces of leeches, treatment (6) 25 pieces of leeches. Reproductive leeches were kept in a water-moss environment in tanks containing 50 liters of chlorine-free water at 28-27°c. The mosses were controlled and sprayed twice a week, and the cocoons inside the mosses were collected and stored at 25°c. Productive leeches were kept in a 10-liter plastic container in the soil of Peat mosss. The peat moss soil was inspected 3 times a week and sprayed to retain moisture, and cocoons were collected inside the moss soil and stored at 25 °c in the peat moss soil. Results: The results showed that the number of cocoons produced in water-moss and paet moss soil did not differ significantly (p <0.05). The weight of the cocoons produced in the two environments did not differ significantly (p <0.05). Also, the number of casualties of productive leeches in the two environments showed no significant cifference (p <0.05).
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