مطالعه تاثیر اسانس مرزه خوزستانی (Satureja khuzestanica) و جاذب سم پلیذورب بر آفلاتوکسیکوزیس تجربی در بلدرچین ژاپنی
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
هما آراک
1
,
محمد امیر کریمی ترشیزی
2
,
شعبان رحیمی
3
1 - دانشجوی کارشناسی ارشد پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران.
2 - استادیار گروه پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران.
3 - استاد گروه پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران
تاریخ دریافت : 1392/09/17
تاریخ پذیرش : 1392/12/12
تاریخ انتشار : 1392/09/01
کلید واژه:
آفلاتوکسین,
کبد,
آسیبشناسی,
مرزه خوزستانی,
پلی ذورب,
چکیده مقاله :
در این تحقیق پتانسیل ضد آفلاتوکسینی تغذیه با اسانس مرزه و جاذب سم پلی ذورب در برابر عوارض آفلاتوکسیکوزیسB1 تجربی مورد بررسی قرار گرفت.تعداد144 قطعه بلدرچین نر ژاپنی 21 روزهبهشش گروه آزمایشی شامل: A- شاهد منفی (جیره بدون آفلاتوکسین)، B- شاهد مثبت (جیره آلوده حاوی 5/2 میلی گرم در کیلوگرم آفلاتوکسین)، C- جیره آلوده+PPM300اسانس مرزه، D- جیره آلوده+PPM600 اسانس مرزه، E- جیره آلوده+PPM900 اسانس مرزه، F- جیره آلوده+g/kg5/2 پلی ذوربباچهار تکرار (هر تکرار ششپرنده) تقسیم شدند و آزمایش درقالب طرح کاملاًتصادفی انجام گرفت. طول دوره آزمایش 10هفته و در انتهای هفته های چهارم و دهم از هر قفس دو قطعه پرنده به طور تصادفی کشتار شدند. وزن نسبی کبد، بورس و طحال اندازه گیری شد و نمونه های بافتی مناسب از کبد تهیه گردید. در پایان هفته دهم سطح مالون دی آلدئید موجود در گوشت تازه و نگه داری شده در یخچال مورد ارزیابی قرار گرفت. استفاده از اسانس مرزه در سطح PPM600موجب کاهش اثرات آفلاتوکسین بر وزن بورس فابرسیوس و طحال گردید.تغییرات چربی بسیار شدید و کانون های گرد و با اندازه متنوع در سلول های کبدی در حال دژنره شدن و فیبروز نواحی باب در اثر وجود آفلاتوکسین (گروه B) مشاهده گردید.به نظر می رسد که استفاده از اسانس مرزه خوزستانی در سطحPPM600و جاذب سم پلی ذورب می تواند به طور موثری اثراتمسمومیت ناشی از آفلاتوکسین B1 را کاهش دهد.
چکیده انگلیسی:
Anti-aflatoxicosis potential of dietary supplementation of savory essential oil and a commercial mycotoxin binder (Polysorb) was investigated by study of some internal organ weights, lipid oxidation of meat and liver lesions. A total of 144 male 21 days old Japanese quails were divided into six treatments and 4 replicates (6 birds in each replication)and treated for 10 wks as follows: A-Negative control, B-Positive control-fed diet contaminated with aflatoxin B1 (2.5 PPM), C- Contaminated feed+300 PPM savory essential oil,D-Contaminated feed+ 600 PPM savory essential oil, E- Contaminated feed+ 900 PPM savory essential oil, F- Contaminated feed+ 2.5 g/kg Polysorb. At the end of the fourth and tenth weeks of experiment, two birds from each cage were randomly euthanized and relative weights of liver, spleen and bursa offabricius were determined and tissue samples of the liver were taken. The lipid oxidation in fresh and refrigerator meat samples were determined at the end of experiment. Use of 600 PPM of savory essential oil reduced the effects of aflatoxin on weights of bursa of fabricius and spleen. Highly intense fatty changes, large and small foci of hepatocellular degeneration and portal fibrosis caused by aflatoxin were observed in non-treated aflatoxicosis induced birds (group B). It seems that dietary supplementation of 600 PPM of savory essential oil and Polysorb can effectively reduce the toxic effects of aflatoxin B1 in Japanese quails.
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