ارزیابی تأثیر ژل گیاه صبر زرد (Aloe vera) بر ساختار بافتی تخمدان موش‌های صحرایی دریافت‌کننده نیکوتین
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
علی محمد شاهین
1
,
علی محمد بهرامی
2
,
عارف دلخوش
3
,
نعمت الله شاکرمی
4
1 - دانش‌آموخته کارشناسی‌ارشد بافت‌شناسی، دانشکده پیرادامپزشکی، دانشگاه ایلام، ایلام، ایران.
2 - دانشیار گروه بافت‌شناسی و میکروب‌شناسی، دانشکده پیرادامپزشکی، دانشگاه ایلام، ایلام، ایران.
3 - دانشجوی دکترای تخصصی آسیب‌شناسی دامپزشکی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران.
4 - کارشناس علوم آزمایشگاهی، دانشکده پیرادامپزشکی، دانشگاه ایلام، ایلام، ایران.
تاریخ دریافت : 1400/07/28
تاریخ پذیرش : 1400/11/19
تاریخ انتشار : 1400/11/01
کلید واژه:
تخمدان,
موش صحرائی,
نیکوتین,
آلوئه‌ورا,
چکیده مقاله :
نیکوتین باعث کاهش فعالیت های تولید مثلی و نیز باروری می شود که عملکرد آن از طریق مهار آزادسازی پروژسترون در فاز لوتئال صورت می گیرد. گیاه صبر زرد یا آلوئه‌ورا (Aloe vera) یکی از گیاهان دارویی با نام علمی (Aloe barbadensis) و متعلق به خانواده آسفودلاسه ست که با داشتن خواص دارویی بسیار زیاد نظیر آنتی اکسیدان، ضد التهاب و ضد پیری مورد توجه بوده است. در مطالعه حاضر اثر محافظتی ژل گیاه مذکور بر تغییرات بافتی تخمدان موش‌های صحرائی دریافت کننده نیکوتین مورد بررسی قرار گرفت. بدین منظور، تعداد 32 سر موش صحرایی ماده نژاد ویستار به 4 گروه شاهد (بدون تیمار)، تیمار با نیکوتین (6 هفته نیکوتین به میزان mg/kg 2/0 وزن حیوانات/تزریق داخل صفاقی)، تیمار با نیکوتین+ آلوئه‌ورا شامل 6 هفته تیمار (با نیکوتین مشابه گروه 2 و ژل گیاه آلوئه‌ورا به میزان mg/kg 400 وزن حیوانات/خوراکی) و تیمار فقط با ژل گیاه آلوئه‌ورا (6 هفته مشابه گروه 3) تقسیم شدند. در پایان دوره مطالعه، موش‌ها با تزریق کتامین-زایلازین آسان‌کشی شده و تخمدان آن ها جهت تهیه مقاطع بافتی و انجام رنگ‌آمیزی هماتوکسیلین- ائوزین به آزمایشگاه منتقل گردید. پس از تیمار با نیکوتین، کاهش وزن بدن، وزن تخمدان، تعداد فولیکول‌های سالم و فعال، جسم زرد جدید و نیز افزایش تعداد فولیکول‌های آترتیک قابل مشاهده بود. همچنین مصرف ژل گیاه آلوئه‌ورا توانست به طور معنی داری تمامی عوارض و تغییرات میکروسکوپی و ماکروسکوپی ناشی از تزریق نیکوتین را بهبود بخشد (05/0p<). نتایج مطالعه حاضر نشان داد که مصرف خوراکی ژل آلوئه‌ورا می‌تواند موجب کاهش و یا مهار اثرات مضر نیکوتین بر بافت تخمدان موش صحرایی شود.
چکیده انگلیسی:
Exposure to nicotine and cigarette smoke has dangerous effects on pregnant women, fetal and neonatal health, and fertility rates. Aloe vera is one of the few medicinal plants that has maintained its popularity for a long time due to its many medicinal properties. Therefore, in the present study, the protective effect of aloe vera gel on ovarian histological changes in nicotine-receiving rats was investigated. For this purpose, 32 female Wistar rats into 4 control groups (normal diet), nicotine (6 weeks nicotine at 0.2 mg / kg body weight of animals injected intra peritoneal), nicotine-aloe vera (6 weeks nicotine injection + gel) Aloe vera plant (400 mg / kg body weight of animals orally), Aloe vera group (6 weeks aloe vera gel by the above method and amount) were divided. At the end of the course, the mice were facilitated and the ovaries were transferred to a histological laboratory to prepare a tissue slide and perform hematoxylin-eosin staining. The results showed that after nicotine treatment, weight loss, ovarian weight, number of healthy and active follicles, new corpus luteum and increase in the number of atretic follicles were observed. But the use of aloe vera gel was able to improve all the microscopic complications and disorders and macroscopic changes caused by nicotine injection. Therefore, oral consumption of aloe vera gel can reduce or inhibit the harmful effects of nicotine in tobacco or drugs on the ovaries and fertility in women.
منابع و مأخذ:
Afrogh, M., Erfani Majd, N. and Najafzadeh Varzi, H. (2016). Morphometric and immunocytochemical study of the quality and maturity of oocytes of diabetic laboratory mice following aloe vera administration. Urmia Medical Journal, 27(3): 186-178. [In Persian]
Arabi, M. (2004). Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia, 36(5): 305-310.
Amo Oghli Tabrizi, B., Mohajeri, D., Balila, A., Rezaei, A.and Mesgari, M. (2006). Evaluation of serum levels of calcium, phosphorus, alkaline phosphatase, magnesium, total protein and albumin after complete removal of ovaries in rats. Journal of Veterinary Clinical Pathology, 1(1): 49-56. [In Persian]
Aydos, K., Gueven, M.C., Can. B. and Ergue, A. (2001). Nicotine toxicity to the ultrastructure of the testis in rats. BJUInternational,88(6): 622-626.
Azad, F., Nejati, V., Shalizar-Jalali, A., Najafi, Gh. and Rahmani, F. (2018). Royal jelly protects male mice against nicotine-induced reproductive failure. Veterinary Research Forum, 9(3): 231-238.
Bahrami Tapehbor, M., Mazaheri, Y., Khaksari Mahabadi, M., Fatemi Tabatabai, S.R. and Tabandeh, M.R. (2016). Aloe vera gel improves the structural disorders of the brain of male rats with streptozotocin-induced diabetes. Journal of Isfahan Medical School, 35(422): 240-235. [In Persian]
Balazadeh Kocheh, F. and Hassanzadeh, Sh. (2014). The effect of nicotine on histomorphology and histomorphometry of ovarian follicles in adult rats. Journal of Qom University of Medical Sciences, 8(6): 9-21. [In Persian]
Bancroft, J.P. and Gamble, M. (2002). Theory and practice of histological techniques. 5th ed., London: New York, pp: 63-84.
Carvalho, C., Favaro, W., Padovani, C. and Cagnon, V. (2006). Morphometric and ultrastructure features of the ventral prostate of rats (Rattus norvegicus) submitted to long‐term nicotine treatment. Andrologia, 38(4): 142-151.
Ghorbani, T., karimi, A., Najafi, Gh., Besharati, M. and Sharafi, M. (2020). Study of the effect of vitagnus castus on maturation and in vitro fertilization of mice with polycystic ovary syndrome. Journal of Veterinary Clinical Pathology, 14(2): 101-113. [In Persian]
Helen, A., Krishnakumar, K., Vijayammal, P.L. and Augusti, K.T. (2000). Antioxidant effect of onion oil(Allium cepa. Linn) on the damagesinduced by nicotine in rats as compared to alpha-tocopherol.Toxicology Letters, 116(1-2): 61-68.
Javan Masoumi, F., Talat Mehrabad, J. and Babri Bonab, R. (2016). Evaluation of aloe vera ethanolic extract on some biomarkers of cardiovascular diseases in mice treated with titanium dioxide nanoparticles. Journal of Animal Research and Development, 9(4): 53-43. [In Persian]
Katzung, B.G. Master, S.B. and Trevor A.J. (2009). Basic and clinical pharmacology. 11th ed., Philadelphia: McGraw Hill, pp: 79-82.
Kosif, R. and Aktas, R.G. (2009). Investigation of the effects of Aloe barbadensis on Rat ovaries: a preliminary study. Journal of Medicinal Food, 12(6): 1393-1397.
Modaresi, M., Khodaei, H. and Khodadi, A. (2013). The effect of aloe vera extract on spermatogenesis and reproductive hormones in mice. Journal of Animal Biology, 6(1): 69-76. [In Persian]
Rahmani Kahnemoei, J. (2016). The effect of passive inhalation of cigarette smoke on serum fat profile in rats. Journal of Veterinary Clinical Pathology, 10(3): 245-250. [In Persian]
Rajasekaran, S., Ravi, K., Sivagnanam, K. and Subramanian, S. (2006). Beneficial effects of aloe vera leafgel extract on lipid profile status in rats withstreptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology, 33(3):232-237.
Saleh, R.A. Agarwal, A., Sharma, R.K. Nelson, D.R. and Thomas, A.J.Jr. (2002). Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertility and Sterility, 78(3): 491-499.
Sistani, S., Raiszadeh, M. and Amiri, A.A. (2017). The effect of different amounts of hydroalcoholic extract of alfalfa on the control of oxidative stress of nicotine in the ovaries of female rats. Journal of Paramedical School of Tehran University of Medical Sciences, 11(3): 3601-351. [In Persian]
Subramanyan, S., Kumar, D.S. and Arulselvan, P. (2006). Wound healing potential of Aloe vera leaf gel studied in experimental rabbit. Asian Journal of Biochemistry, 1(2): 178-85.
Wack, J.T. and Rodin, J. (1982). Smoking and its effects on body weight and systems of caloric regulation. The American Journal of Clinical Nutrition, 35(2): 366-380.
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Afrogh, M., Erfani Majd, N. and Najafzadeh Varzi, H. (2016). Morphometric and immunocytochemical study of the quality and maturity of oocytes of diabetic laboratory mice following aloe vera administration. Urmia Medical Journal, 27(3): 186-178. [In Persian]
Arabi, M. (2004). Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia, 36(5): 305-310.
Amo Oghli Tabrizi, B., Mohajeri, D., Balila, A., Rezaei, A.and Mesgari, M. (2006). Evaluation of serum levels of calcium, phosphorus, alkaline phosphatase, magnesium, total protein and albumin after complete removal of ovaries in rats. Journal of Veterinary Clinical Pathology, 1(1): 49-56. [In Persian]
Aydos, K., Gueven, M.C., Can. B. and Ergue, A. (2001). Nicotine toxicity to the ultrastructure of the testis in rats. BJUInternational,88(6): 622-626.
Azad, F., Nejati, V., Shalizar-Jalali, A., Najafi, Gh. and Rahmani, F. (2018). Royal jelly protects male mice against nicotine-induced reproductive failure. Veterinary Research Forum, 9(3): 231-238.
Bahrami Tapehbor, M., Mazaheri, Y., Khaksari Mahabadi, M., Fatemi Tabatabai, S.R. and Tabandeh, M.R. (2016). Aloe vera gel improves the structural disorders of the brain of male rats with streptozotocin-induced diabetes. Journal of Isfahan Medical School, 35(422): 240-235. [In Persian]
Balazadeh Kocheh, F. and Hassanzadeh, Sh. (2014). The effect of nicotine on histomorphology and histomorphometry of ovarian follicles in adult rats. Journal of Qom University of Medical Sciences, 8(6): 9-21. [In Persian]
Bancroft, J.P. and Gamble, M. (2002). Theory and practice of histological techniques. 5th ed., London: New York, pp: 63-84.
Carvalho, C., Favaro, W., Padovani, C. and Cagnon, V. (2006). Morphometric and ultrastructure features of the ventral prostate of rats (Rattus norvegicus) submitted to long‐term nicotine treatment. Andrologia, 38(4): 142-151.
Ghorbani, T., karimi, A., Najafi, Gh., Besharati, M. and Sharafi, M. (2020). Study of the effect of vitagnus castus on maturation and in vitro fertilization of mice with polycystic ovary syndrome. Journal of Veterinary Clinical Pathology, 14(2): 101-113. [In Persian]
Helen, A., Krishnakumar, K., Vijayammal, P.L. and Augusti, K.T. (2000). Antioxidant effect of onion oil(Allium cepa. Linn) on the damagesinduced by nicotine in rats as compared to alpha-tocopherol.Toxicology Letters, 116(1-2): 61-68.
Javan Masoumi, F., Talat Mehrabad, J. and Babri Bonab, R. (2016). Evaluation of aloe vera ethanolic extract on some biomarkers of cardiovascular diseases in mice treated with titanium dioxide nanoparticles. Journal of Animal Research and Development, 9(4): 53-43. [In Persian]
Katzung, B.G. Master, S.B. and Trevor A.J. (2009). Basic and clinical pharmacology. 11th ed., Philadelphia: McGraw Hill, pp: 79-82.
Kosif, R. and Aktas, R.G. (2009). Investigation of the effects of Aloe barbadensis on Rat ovaries: a preliminary study. Journal of Medicinal Food, 12(6): 1393-1397.
Modaresi, M., Khodaei, H. and Khodadi, A. (2013). The effect of aloe vera extract on spermatogenesis and reproductive hormones in mice. Journal of Animal Biology, 6(1): 69-76. [In Persian]
Rahmani Kahnemoei, J. (2016). The effect of passive inhalation of cigarette smoke on serum fat profile in rats. Journal of Veterinary Clinical Pathology, 10(3): 245-250. [In Persian]
Rajasekaran, S., Ravi, K., Sivagnanam, K. and Subramanian, S. (2006). Beneficial effects of aloe vera leafgel extract on lipid profile status in rats withstreptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology, 33(3):232-237.
Saleh, R.A. Agarwal, A., Sharma, R.K. Nelson, D.R. and Thomas, A.J.Jr. (2002). Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertility and Sterility, 78(3): 491-499.
Sistani, S., Raiszadeh, M. and Amiri, A.A. (2017). The effect of different amounts of hydroalcoholic extract of alfalfa on the control of oxidative stress of nicotine in the ovaries of female rats. Journal of Paramedical School of Tehran University of Medical Sciences, 11(3): 3601-351. [In Persian]
Subramanyan, S., Kumar, D.S. and Arulselvan, P. (2006). Wound healing potential of Aloe vera leaf gel studied in experimental rabbit. Asian Journal of Biochemistry, 1(2): 178-85.
Wack, J.T. and Rodin, J. (1982). Smoking and its effects on body weight and systems of caloric regulation. The American Journal of Clinical Nutrition, 35(2): 366-380.
