مقایسه وضعیت اکسیداتیو سرم اسب‌های سالم عرب و دره‌شور
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
مریم کریمی دهکردی
1
,
ناصر صالحی
2
,
پردیس بنی مهدی
3
1 - استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
2 - دانشجوی دکترای تخصصی داخلی دام بزرگ، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.
3 - دانشجوی دکترای حرفه‌ای، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
تاریخ دریافت : 1399/01/21
تاریخ پذیرش : 1400/02/05
تاریخ انتشار : 1400/02/01
کلید واژه:
اسب,
استرس اکسیداتیو,
آنزیم‌های آنتی‌اکسیدانی,
نژاد عرب,
نژاد دره‌شور,
چکیده مقاله :
استرس اکسیداتیو که عبارت از عدم تعادل بین تولید رادیکال های آزاد اکسیژن و ظرفیت دفاع آنتی اکسیدانی بدن است، به شدت با فعالیت بدنی در طول زندگی ورزشی اسب ها در ارتباط می‌باشد. هدف از انجام مطالعه حاضر، ارزیابی سطوح سرمی مالون‌دی‌آلدئید (malondialdehyde; MDA)، ظرفیت آنتی‌اکسیدانی تام (total antioxidant capacity; TAC) و آنزیم‌های آنتی‌اکسیدانی سوپراکسید دیسموتاز (superoxid dismutase; SOD)، گلوتاتیون پراکسیداز (glutation peroxidase; GPX) و کاتالاز (catalase; CAT) به‌عنوان شاخص‌های استرس اکسیداتیو و مقایسه آن‌ها در دو نژاد عرب و دره شور بود. به این منظور، تعداد 26 رأس اسب ماده غیر آبستن و غیر شیروار از استان یزد (13 رأس نژاد عرب و 13 رأس نژاد دره شور) به طور تصادفی انتخاب شدند. نمونه‌های خونی تمامی اسب ها در زمان استراحت اخذ شد. در سرم اسب های موردمطالعه MDA، TACو آنزیم‌های آنتی‌اکسیدانی مورد سنجش قرار گرفت. نتایج نشان داد که میزان MDA در سرم اسبان نژاد عرب به‌طور معنی‌دار کمتر از مقدار آن در سرم اسب های نژاد دره شور بود (55/0±98/0 در مقابل 56/0±59/1 میکرومول بر لیتر). همچنین غلظت TAC در سرم اسب های نژاد عرب و دره شور هیچ‌گونه اختلاف آماری معنی دار نشان ندادند، هرچند میزان این پارامتر در اسب های نژاد عرب مقداری بالاتر از نژاد دره شور بود (75/70±54/113 در مقابل 93/53±56/112 میکرومول بر لیتر). سطح آنزیم‌های SOD، GPX و CAT نیز در اسبان نژاد عرب بالاتر از نژاد دره شور بود که این اختلاف در آنزیم‌های SOD و GPX از نظر آماری معنی‌دار بود (05/0>p). بالا بودن آنزیم‌های آنتی‌اکسیدانی و پایین بودن سطح MDA نشان‌دهنده وضعیت سلامت بهتر اسب‌های نژاد عرب می‌باشد و به نظر می رسد نژاد و ژنتیک در افزایش قدرت سیستم دفاع آنتی اکسیدانی نقش داشته باشد.
چکیده انگلیسی:
Oxidative stress, which is an imbalance between the production of oxygen free radicals and the body's antioxidant defense, is strongly associated with physical activity during a horse's sporting life. The aim of the present study was to evaluate the serum levels of Malondialdehyde (MDA), total antioxidant capacity (TAC) and the antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxatase (GPX) and catalase (CAT) as indicators of oxidative stress and to compare them between two horse races of Arab and Dareshoor. For this purpose, 26 non-pregnant and non-lactating female horses from Yazd province (13 Arab and 13 Dareshoor) were randomly selected. Blood samples were taken from all horses at rest. MDA, TAC and antioxidant enzymes were tested in the serum of the studied horses. The results showed that the rate of MDA in the serum of Arabian horses was significantly lower than Dareshoor horses (0.98±0.55 vs 1.59±0.56 µmol/L, respectively). Also, serum TAC concentrations in Arabian and Dareshoor horses did not show any statistically significant difference, although the rate of this parameter in Arabian horses was slightly higher than in Dareshoor (113.54±70.75 vs 112.56±53.93 µmol/L, respectively). In Arab race, level of SOD, GPX and CAT were also higher than Dareshoor. These differences between SOD and GPX enzymes were statistically significant (p<0.05). High levels of antioxidant enzymes and low levels of MDA indicate better health status for Arabian horses, and breed and genetics appear to play a role in enhancing the strength of the antioxidant defense system.
منابع و مأخذ:
Abbasali Pourkabireh, M., Atyabi, N., Mojabi, A., Nadalian, M., Nowrouzian, I., Khazrainia, P., et al. (2000). A survey for biochemical pattern of Caspian Miniature ponies and comparing with Iranian Arab horses. Journal of the Faculty of Veterinary Medicine, University of Tehran, 55(2): 37-41.
Basit, A., Ali, M., Hussain, G., Irfan, S., Saqib, M., Iftikhar, A., et al. (2020). Effect of Equine Piroplasmosis on hematological and oxidative stress biomarkers in relation to different seasons in district Sargodha, Pakistan. Pakistan Veterinary Journal, 40(1): 43-48.
Bażanów, B.A., Chełmecka, E., Romuk, E. and Stygar, D.M. (2020). Basic studies on the oxidative stress markers in two types of Horse breed: Semi-isolated population of Huculs is different from commercially used Arabian Horses. BioMed Research International, 2020: 1-10.
Benzie, I.F. and Strain, J.J. (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in enzymology, 299: 15-27.
Bottegaro, N.B., Gotić, J., Šuran, J., Brozić, D., Klobučar, K., Bojanić, K., et al. (2018). Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Veterinary Research, 14(1): 1-9.
Burton, G. J. and Jauniaux, E. (2011). Oxidative stress. Best Practice and Research Clinical Obstetrics and Gynaecology, 25(3): 287-299.
Crimi, E., Sica, V., Williams-Ignarro, S., Zhang, H., Slutsky, A. S., Ignarro, L. J., et al. (2006). The role of oxidative stress in adult critical care. Free Radical Biology and Medicine, 40(3): 398-406.
Crowley, J., Po. E., Celi, P. and Muscatello, G. (2013). Systemic and respiratory oxidative stress in the pathogenesis and diagnosis of Rhodococcus equi pneumonia. Equine Veterinary Journal, 45(S45): 20-25.
D'autréaux, B. and Toledano, M. B. (2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8(10): 813-824.
Deaton, C.M. (2006). The role of oxidative stress in an equine model of human asthma. Redox Report, 11(2): 46-52.
Deger, S., Deger, Y., Bicek, K., Ozdal, N. and Gul, A. (2009). Status of Lipid peroxidation, antioxidants and oxidation products of Nitric Oxide in Equine Babesiosis: Status of antioxidant and oxidant in Equine Babesiosis. Journal of Equine Veterinary Science, 29(10): 743-747.
El-Deeb, W.M. and El-Bahr, S.M. (2010). Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers. Veterinary Research Communications, 34(8): 677-689.
Firouz, L. (1998). The original ancestors of the Turkoman, Caspian horses. Proc. 1st Int. Conference on Turkoman Horse, Ashgabad, Turkmenistan.
Grotto, D., Santa Maria, L.D., Boeira, S., Valentini, J., Char, M.F., Moro, A.M., et al. (2007) Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography–visible detection. Journal of Pharmaceutical and Biomedical Analysis, 43(2): 619-624.
Kirschvink, N. and Lekeux, P. (2002). Oxidative stress in equine medicine-Current knowledge. Pferdeheilkunde, 18(6): 569-573.
Kirschvink, N., De Moffarts, B. and Lekeux, P. (2008). The oxidant/antioxidant equilibrium in horses. The Veterinary Journal, 177(2): 178-191.
Kirschvink, N., De Moffarts, B., Farnir, F., Pincemail, J. and Lekeux, P. (2006). Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds. Equine Veterinary Journal, 38(S36): 239-244.
Kuloglu, M., Atmaca, M., Tezcan, E., Ustundag, B. and Bulut, S. (2002). Antioxidant enzyme and malondialdehyde levels in patients with panic disorder. Neuropsychobiology, 46(4): 186-189.
Lacerda, L., Campos, R., Sperb, M., Soares, E., Barbosa , P., Godinho, E., et al. (2006). Hematologic and biochemical parameters in three high performance horse breeds from Southern Brazil. Archives of Veterinary Science, 11(2): 40-44.
Leeuwenburgh, C. and Heinecke, J. (2001). Oxidative stress and antioxidants in exercise. Current Medicinal Chemistry, 8(7): 829-838.
Lu, X., Wang, C. and Liu, B. (2015). The role of Cu/Zn-SOD and Mn-SOD in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Fish and Shellfish Immunology, 42(1): 58-65.
Mami, S., Khaje, G., Shahriari, A. and Gooraninejad, S. (2019). Evaluation of biological indicators of Fatigue and Muscle damage in Arabian horses after race. Journal of Equine Veterinary Science, 78: 74-78.
Rahman, I., Biswas, S.K. and Kirkham, P.A. (2006). Regulation of inflammation and redox signaling by dietary polyphenols. Biochemical Pharmacology, 72(11): 1439-1452.
Ray, P.D., Huang, B.W. and Tsuji, Y. (2012). Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular Signalling, 24(5): 981-990.
Serafini, M. and Del Rio, D. (2004). Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Report, 9(3): 145-152.
Shono, S., Gin, A., Minowa, F., Okubo, K. and Mochizuki, M. (2020). The oxidative stress markers of horses—the comparison with other animals and the influence of exercise and disease. Animals, 10(4): 1-9.
Strycharz-Dudziak, M., Kiełczykowska, M., Drop, B., Świątek, Ł., Kliszczewska, E., Musik, I., et al. (2019). Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal cancer associated with EBV Infection. Oxidative Medicine and Cellular Longevity, 2019: 1-15.
Van der Kolk, J.H., Thomas, S., Mach, N., Ramseyer, A., Burger, D., Gerber, V., et al. (2020). Serum acylcarnitine profile in endurance horses with and without metabolic dysfunction. The Veterinary Journal, 255: 1-6.
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Abbasali Pourkabireh, M., Atyabi, N., Mojabi, A., Nadalian, M., Nowrouzian, I., Khazrainia, P., et al. (2000). A survey for biochemical pattern of Caspian Miniature ponies and comparing with Iranian Arab horses. Journal of the Faculty of Veterinary Medicine, University of Tehran, 55(2): 37-41.
Basit, A., Ali, M., Hussain, G., Irfan, S., Saqib, M., Iftikhar, A., et al. (2020). Effect of Equine Piroplasmosis on hematological and oxidative stress biomarkers in relation to different seasons in district Sargodha, Pakistan. Pakistan Veterinary Journal, 40(1): 43-48.
Bażanów, B.A., Chełmecka, E., Romuk, E. and Stygar, D.M. (2020). Basic studies on the oxidative stress markers in two types of Horse breed: Semi-isolated population of Huculs is different from commercially used Arabian Horses. BioMed Research International, 2020: 1-10.
Benzie, I.F. and Strain, J.J. (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in enzymology, 299: 15-27.
Bottegaro, N.B., Gotić, J., Šuran, J., Brozić, D., Klobučar, K., Bojanić, K., et al. (2018). Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Veterinary Research, 14(1): 1-9.
Burton, G. J. and Jauniaux, E. (2011). Oxidative stress. Best Practice and Research Clinical Obstetrics and Gynaecology, 25(3): 287-299.
Crimi, E., Sica, V., Williams-Ignarro, S., Zhang, H., Slutsky, A. S., Ignarro, L. J., et al. (2006). The role of oxidative stress in adult critical care. Free Radical Biology and Medicine, 40(3): 398-406.
Crowley, J., Po. E., Celi, P. and Muscatello, G. (2013). Systemic and respiratory oxidative stress in the pathogenesis and diagnosis of Rhodococcus equi pneumonia. Equine Veterinary Journal, 45(S45): 20-25.
D'autréaux, B. and Toledano, M. B. (2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8(10): 813-824.
Deaton, C.M. (2006). The role of oxidative stress in an equine model of human asthma. Redox Report, 11(2): 46-52.
Deger, S., Deger, Y., Bicek, K., Ozdal, N. and Gul, A. (2009). Status of Lipid peroxidation, antioxidants and oxidation products of Nitric Oxide in Equine Babesiosis: Status of antioxidant and oxidant in Equine Babesiosis. Journal of Equine Veterinary Science, 29(10): 743-747.
El-Deeb, W.M. and El-Bahr, S.M. (2010). Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers. Veterinary Research Communications, 34(8): 677-689.
Firouz, L. (1998). The original ancestors of the Turkoman, Caspian horses. Proc. 1st Int. Conference on Turkoman Horse, Ashgabad, Turkmenistan.
Grotto, D., Santa Maria, L.D., Boeira, S., Valentini, J., Char, M.F., Moro, A.M., et al. (2007) Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography–visible detection. Journal of Pharmaceutical and Biomedical Analysis, 43(2): 619-624.
Kirschvink, N. and Lekeux, P. (2002). Oxidative stress in equine medicine-Current knowledge. Pferdeheilkunde, 18(6): 569-573.
Kirschvink, N., De Moffarts, B. and Lekeux, P. (2008). The oxidant/antioxidant equilibrium in horses. The Veterinary Journal, 177(2): 178-191.
Kirschvink, N., De Moffarts, B., Farnir, F., Pincemail, J. and Lekeux, P. (2006). Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds. Equine Veterinary Journal, 38(S36): 239-244.
Kuloglu, M., Atmaca, M., Tezcan, E., Ustundag, B. and Bulut, S. (2002). Antioxidant enzyme and malondialdehyde levels in patients with panic disorder. Neuropsychobiology, 46(4): 186-189.
Lacerda, L., Campos, R., Sperb, M., Soares, E., Barbosa , P., Godinho, E., et al. (2006). Hematologic and biochemical parameters in three high performance horse breeds from Southern Brazil. Archives of Veterinary Science, 11(2): 40-44.
Leeuwenburgh, C. and Heinecke, J. (2001). Oxidative stress and antioxidants in exercise. Current Medicinal Chemistry, 8(7): 829-838.
Lu, X., Wang, C. and Liu, B. (2015). The role of Cu/Zn-SOD and Mn-SOD in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Fish and Shellfish Immunology, 42(1): 58-65.
Mami, S., Khaje, G., Shahriari, A. and Gooraninejad, S. (2019). Evaluation of biological indicators of Fatigue and Muscle damage in Arabian horses after race. Journal of Equine Veterinary Science, 78: 74-78.
Rahman, I., Biswas, S.K. and Kirkham, P.A. (2006). Regulation of inflammation and redox signaling by dietary polyphenols. Biochemical Pharmacology, 72(11): 1439-1452.
Ray, P.D., Huang, B.W. and Tsuji, Y. (2012). Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular Signalling, 24(5): 981-990.
Serafini, M. and Del Rio, D. (2004). Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Report, 9(3): 145-152.
Shono, S., Gin, A., Minowa, F., Okubo, K. and Mochizuki, M. (2020). The oxidative stress markers of horses—the comparison with other animals and the influence of exercise and disease. Animals, 10(4): 1-9.
Strycharz-Dudziak, M., Kiełczykowska, M., Drop, B., Świątek, Ł., Kliszczewska, E., Musik, I., et al. (2019). Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal cancer associated with EBV Infection. Oxidative Medicine and Cellular Longevity, 2019: 1-15.
Van der Kolk, J.H., Thomas, S., Mach, N., Ramseyer, A., Burger, D., Gerber, V., et al. (2020). Serum acylcarnitine profile in endurance horses with and without metabolic dysfunction. The Veterinary Journal, 255: 1-6.
