تاثیر سطوح مختلف سرکه سیب بر آنزیم¬های کبدی و هیستوپاتولوژی روده جوجه¬های گوشتی
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکیحسنیه حیدری 1 , محمد جهانتیغ 2 , سیده آیدا داوری 3 , داریوش سعادتی 4
1 - دانش¬آموخته دکترای عمومی دامپزشکی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
2 - استاد گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
3 - استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
4 - دانشیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
کلید واژه: آنزیم¬های کبدی, جوجههای گوشتی, سرکه سیب, هیستوپاتولوژی روده.,
چکیده مقاله :
امروزه به منظور افزایش بازده غذایی در صنعت مرغداری، از مواد افزودنی از جمله سرکه در جیره استفاده میشود. هدف از انجام پژوهش حاضر، بررسی تاثیر سطوح مختلف سرکه سیب در جیره غذایی جوجههای گوشتی بر فعالیت آنزیمهای کبدی و هیستوپاتولوژی روده است. بدین منظور تعداد 64 قطعه جوجه گوشتی نژاد راس 308 بهطور تصادفی به 4 گروه آزمایشی با 4 تکرار و 4 جوجه در هر تکرار تقسیم شدند. جیره غذایی در گروههای 2، 3 و 4 به ترتیب با مقادیر 1، 2 و 3 درصد سرکه سیب مخلوط شد و گروه 1، با جیره معمولی تغذیه گردید. در روزهای 14 و 28 دوره پرورش، یک جوجه از هر گروه به صورت تصادفی انتخاب گردید و خونگیری از ورید بال آنها انجام و فعالیت آنزیمهای کبدی سرم با استفاده از دستگاه اتوآنالایزر(Auto Analyzer) و کیت اختصاصی بررسی شد. برای بررسی تغییرات هیستوپاتولوژیکی روده، نمونههای بافتی از قسمت ژژنوم روده باریک تهیه شد. طبق نتایج، میزان آنزیم ALT (alanine aminotransfrase) در گروههای 3 و 4، بهطور معنیداری کمتر از گروه کنترل بود (05/0<p) ولی سرکه سیب، تغییرات معنیداری در میزان آنزیمهای ALP(alkaline phosphatase) و AST (aspartate aminotransferase) ایجاد نکرد. نتایج هیستوپاتولوژی ژژنوم هم نشان دهنده افزایش ارتفاع پرزهای روده، عمق کریپتهای لیبرکوهن و تعداد پرزهای روده در گروههای دریافت کننده سرکه سیب بود. براسا یافته‌های ما، میتوان نتیجهگیری کرد که سرکه سیب، سبب کاهش فعالیت برخی آنزیمهای کبدی، افزایش سطح پرزها در ژژنوم روده باریک و در نتیجه بهبود عملکرد روده میگردد.
Nowadays, to increase the food efficiency in the poultry industry, additives such as vinegar are used in the diet. The purpose of this research was to investigate the effect of different levels of apple vinegar in the diet of broiler chickens on the activity of liver enzymes and intestinal histopathology. For this purpose, 64 pieces of Ross 308 broiler chickens were randomly divided into 4 experimental groups. The diet in groups 2, 3, and 4 was mixed with 1, 2, and 3 percent apple vinegar, respectively, and group 1 was fed with normal diet. On the 14th and 28th days, one chick was randomly selected from each replication. Blood samples were collected from the brachial vein. Then, the activity of liver enzymes was checked using an Auto Analyzer and a dedicated kit. To observe intestinal histopathological changes, tissue samples were prepared from the jejunum. According to the results, the amount of Alanine aminotransferase (ALT) enzyme in groups 3 and 4 was significantly lower than the control group (p<0.05). However, there were no significant changes in the amount of Alkaline phosphatase (ALP) and Aspartate aminotransferase (AST). The results of histopathology of the jejunum showed an increase in the height of intestinal villi, the depth of Lieberkohn's crypts and the number of intestinal villi in the groups receiving apple vinegar. Based on our findings, it can be concluded that apple vinegar reduces the activity of some liver enzymes, increases the surface of villi in the jejunum, and thus improves intestinal function.
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