Exploring the Impact of Seasonal Sampling, Media Phase, Concentration and Type of Hormone on the Micropropagation of Dog Rose (Rosa canina L.)
محورهای موضوعی : مجله گیاهان زینتیMorteza Sheikh-Assadi 1 , Neda Ghaffari Tari 2 , Roohangiz Naderi 3 , Reza Fattahi 4
1 - Department of Horticultural Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran
2 - Department of Horticultural Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran
3 - Department of Horticultural Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran
4 - Department of Horticultural Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran
کلید واژه: tissue culture, Proliferation, Rooting, establishment, Disinfection, Nodal explant,
چکیده مقاله :
One of the most significant decorative and medicinal plants, the dog rose (Rosa canina L.), serves as the rootstock for the majority of ornamental roses, including hybrid roses. The quick growth with desirable traits and the creation of wholesome, disease-free plants are the major outcomes of in vitro rose propagation. So, in order to provide micropropagation instructions for this plant utilizing nodal explants, this research was carried out. Many factors including the type, concentration, and phase of culture medium, sampling season, type, and concentration of hormones were investigated in several experiments. Explant disinfection was significantly affected by the timing of immersion in different sodium hypochlorite concentrations as well as the explant sampling season. The explants harvested in the winter and treated with 1% sodium hypochlorite showed the lowest rate of infection and the best percentage of survival. The percent of explant establishment was affected by the culture medium used as well as the IBA and BAP concentrations employed; the highest percentage of explant establishment was found in the MS culture medium with 0.3 mg L-1 BAP and or 0.4 mg L-1 IBA. The solid MS culture medium with 0.5 mg L-1 BAP had a stronger impact on proliferation. The results of the root formation test proved that the highest percentage of rooting is obtained in the half-strength MS culture medium with 2 mg L-1 IBA. After adapting to the environment with more than 80% survival, the produced plants were finally moved to the greenhouse. Using these findings, Rosa canina can be quickly commercially propagated for use in breeding and conservation efforts.
نسترن وحشی (Rosa canina L) یکی از مهم‌ترین گیاهان زینتی و دارویی، به عنوان پایه برای اکثر رزهای زینتی از جمله رزهای هیبرید بکار می‌رود. رشد سریع همراه با صفات مطلوب و ایجاد گیاهان سالم و عاری از بیماری از نتایج اصلی تکثیر درون‌شیشه‌ای رز است. از اینرو این پژوهش به منظور ارائه دستورالعمل ریزازدیادی این گیاه با استفاده از ریزنمونه‌های گره انجام شد. عوامل زیادی از جمله نوع، غلظت و حالت محیط کشت، فصل نمونه‌برداری، نوع و غلظت تنظیم‌کننده‌های رشد در چندین آزمایش مورد بررسی قرار گرفت. ضدعفونی ریزنمونه به طور قابل توجهی تحت تاثیر زمان غوطه‌وری در غلظت‌های مختلف هیپوکلریت سدیم و همچنین فصل نمونه‌برداری ریزنمونه قرار گرفت. ریزنمونه‌های برداشت شده در زمستان که با هیپوکلریت سدیم یک درصد تیمار شده بودند کمترین میزان آلودگی و بیشترین درصد زنده‌مانی را نشان دادند. درصد استقرار ریزنمونه تحت تأثیر محیط کشت و همچنین غلظت IBA و BAP قرار گرفت. بالاترین درصد استقرار ریزنمونه در محیط کشت MS دارای 0.3 میلی‌گرم در لیتر BAP و یا 0.4 میلی‌گرم در لیتر IBA بدست آمد. محیط کشت جامد MS با 0.5 میلی‌گرم در لیتر BAP تأثیر زیادی بر پرآوری داشت. نتایج آزمایش ثابت کرد که بیشترین درصد ریشه‌زایی در محیط کشت ½ MS با 2 میلی‌گرم در لیتر IBA به دست می‌آید. پس از سازگاری (با بیش از 80 درصد زنده‌مانی)، در نهایت گیاهان تولید شده به گلخانه منتقل شدند. با استفاده از یافته‌های حاضر، نسترن وحشی را می‌توان به سرعت به صورت تجاری برای اهداف اصلاحی و حفاظتی تکثیر نمود.
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