Physiological Changes During Cold Storage of Lilium ledebourii (Baker) Boiss. Bulb, a Rare Indigenous Species to Iran
محورهای موضوعی : مجله گیاهان زینتی
Fatemeh Abazarifard
1
(Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Seyed Najmmaddin Mortazavi
2
(Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran)
Rohangiz Naderi
3
(Department of Horticultural Sciences, Faculty of Agricultural Science and Engineering, University of Tehran, Tehran, Iran)
Sepideh Kalateh Jari
4
(Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran)
کلید واژه: Hormone, Saponin, Lily, Soluble sugars, Cold period,
چکیده مقاله :
Lilium ledebourii is a native and endangered rare species in Iran. It requires a cold period for proper growth and flowering. This experiment was performed to investigate the effects of cold storage on changes of endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA3), sucrose, fructose, glucose, and total saponin during dormancy of Lilium ledebourii bulbs. The bulbs of lily were stored at 1 or 7 ºC, for 0, 30, 60, 90, and 120 days, and after each period removed from storage and analyzed using HPLC system. The results indicated that, cold storage at 1 ºC for 120 days decreased the levels of ABA while, 7 ºC in the same duration increased its content, slightly. The levels of IAA increased at 1 ºC storage, while remained almost constant at 7 ºC. Cold treatment at 1 ºC increased the levels of GA3, while storage at 7 ºC caused slight decrease in its content. Sucrose and fructose concentrations increased in both temperatures during the storage, while minor reduction in glucose content was observed. Storage at 7 ºC for 120 days induced the highest content of total saponin. The magnitude of changes in hormones and soluble sugars measured at 1 ºC was greater than that at 7 ºC.
سوسن چلچراغ یکی از گونه­های نادر بومی و در معرض خطر انقراض است که برای رشد و گلدهی مناسب نیاز به یک دوره سرمایی دارد. این آزمایش به­منظور بررسی اثرات سرمادهی پیاز بر تغییرات تنظیم کننده­های رشد درون­زای آبسیزیک اسید (ABA)، ایندول استیک اسید (IAA)، جیبرلیک اسید (GA3) و قندهای محلول ساکارز، فروکتوز، گلوکز و همچنین ساپونین تام در طول خواب پیاز سوسن چلچراغ انجام شد. بدین­منظور پیازهای این گیاه در دمای 1 یا 7 درجه سانتی­گراد به مدت 0، 30، 90،60 و 120 روز قرار گرفتند و پس از پایان هر دوره، از انبار برداشت و توسط دستگاه HPLC تجزیه و تحلیل شدند. نتایج نشان داد که سرمادهی پیازها در یک درجه سانتی­گراد به مدت 120 روز باعث کاهش سطح ABA شد در حالیکه 7 درجه سانتی­گراد در همان مدت زمان سبب افزایش جزیی در سطح این هورمون شد. تیمارهای سرمایی یک درجه سانتی­گراد سبب افزایش سطح IAA و GA3 شدند اما در 7 درجه سانتی­گراد سطح IAA تقریبا ثابت باقی ماند و GA3 کاهش جزئی نشان داد. غلظت­های ساکارز و فروکتوز در هر دو تیمار دمایی افزایش یافتند در حالیکه مقدار گلوکز کاهش یافت. انبار پیازها در 7 درجه سانتی­گراد به مدت 120 روز بالاترین سطح ساپونین تام را سبب شد. تغییرات هورمون­ها و قندهای اندازه گیری شده در 1 درجه سانتی­گراد بیشتر از 7 درجه سانتی­گراد بود.
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