Changes in Total Phenol and Some Enzymatic and Non-Enzymatic Antioxidant Activities of Rose-scented Geranium (Pelargonium graveolens) in Response to Exogenous Ascorbic Acid and Iron Nutrition
محورهای موضوعی : مجله گیاهان زینتیAbdolhossein Rezaei Nejad 1 , Zeinab Izadi 2 , Kobra Sepahvand 3 , Hassan Mumivand 4 , Sadegh Mousavifard 5
1 - Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, PO box 465, Korramabad, Iran
2 - Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, PO box 465, Korramabad, Iran
3 - Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, PO box 465, Korramabad, Iran
4 - Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, PO box 465, Korramabad, Iran
5 - Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, PO box 465, Korramabad, Iran
کلید واژه: ascorbate peroxidase, Catalase, peroxidase, Essential oil,
چکیده مقاله :
The strong antioxidant activity of Pelargonium graveolens is well established. The question addressed in this study was whether different concentrations of exogenous ascorbic acid (AsA) and iron (Fe) could influence the antioxidant activity and total phenol content (TPC) of geranium. Thus, three levels of Fe (0, 20 and 40 µM) and three levels of AsA (0, 1 and 2 mM) in the nutrient solution were combined factorially based on a completely randomized design with six replications, and chlorophyll content, TPC, and antioxidant activities of the leaves were measured. The results showed that oil content, ascorbate peroxidase (APX), and catalase (CAT) activities were increased in leaf samples under Fe starvation, regardless of the AsA concentration. The highest peroxidase (POD) activity was observed in samples treated with 20 µM Fe and 1 mM AsA. The highest total chlorophyll content was produced in plants treated with 40 µM Fe along with 1 mM AsA. TPC was increased with an increase in Fe concentration. Despite the positive effect of AsA on the pigment contents, plants treated with AsA showed lower TPC under all Fe concentrations. In total, lower Fe nutrition increased oil content and reactive oxygen species (ROS) scavenging activity of geranium. AsA application increased oil content while decreased total phenol and antioxidant activity in this plant.
فعالیت آنتیاکسیدانی قوی حاصل از اسانس و عصاره شمعدانی عطری بسیار مشهور است. سوالی که در این تحقیق مطرح شده است این است که آیا غلظتهای مختلف آسکوربیک اسید خارجی و آهن میتوانند فعالیت آنتیاکسیدانی و میزان فنول کل را در شمعدانی عطری تحت تاثیر قرار دهند. بدینترتیب، سه سطح از آهن (0، 20 و 40 میکرومولار) و سه سطح از آسکوربیک اسید (0، 1، 2 میلی مولار) در محلول غذایی در قالب آزمایش فاکتوریل بر پایه کاملا تصادفی، با 6 تکرار ترکیب شدند و میزان کلروفیل، فنول کل و فعالیت آنزیمهای آنتیاکسیدانی در برگها اندازهگیری شد. نتایج نشان داد که میزان اسانس، فعالیت آسکوبات پراکسیداز و کاتالاز در برگهای گیاهان تحت شرایط فقر آهن بدون توجه به غلظت آسکوربیک اسید افزایش نشان داد. بالاترین فعالیت پراکسیداز در نمونههایی که تحت تیمار 20 میکرومولار آهن با یک میلی مولار آسکوربیک اسید قرار داشتند، مشاهده شد. فنول کل با افزایش غلظت آهن افزایش پیدا کرد. علی رغم اثر مثبت آسکوربیک اسید روی میزان رنگریزهها، گیاهان تیمار شده با آسکوربیک اسید میزان فنول کل کمتری را در کلیه غلظتهای آهن بکار رفته نشان دادند. بطورکلی، غلظتهای پایین آهن موجب افزایش میزان اسانس و فعالیت مقابله با رادیکالهای آزاد در این گیاه گردید. آسکوربیک اسید موجب افزایش میزان اسانس شد، درحالیکه موجب کاهش فنول کل و فعالیت آنتیاکسیدانی در این گیاه شد.
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