A Simple and Rapid Method for Micropropagation of Petunia × hybrida F1 'Opera Supreme Pink Morn'

محورهای موضوعی : مجله گیاهان زینتی

Shahram Mehri ¹ , Mohammad Nabi Ilkaee ² , Farzin Saeedzadeh ³

1 - Department of Agronomy, Pars Abad Moghan Branch, Islamic Azad University, Pars Abad Moghan, Iran
2 - Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Agronomy and Plant Breeding, Astara Branch, Islamic Azad University, Astara, Iran.

تاریخ دریافت : 1396/12/25 تاریخ پذیرش : 1397/04/23 تاریخ انتشار : 1397/08/10

کلید واژه: tissue culture, plant growth regulators, Auxins, Cytokinins, ornamental plants, &lt, i&gt, In vitro&lt, /i&gt, culture,

چکیده مقاله :

For efficient regeneration of Petunia × hybrida F1 'Opera Supreme Pink Morn', a simple in vitro micropropagation protocol was developed. Axillary bud explants dissected from 30-day-old in vitro-grown seedlings sprouted from hybrid seeds were cultured on Murashige and Skoog (MS) medium supplemented with 36 combinations of 6-benzylaminopurine (BA; 0.00, 0.50, 1.00, 2.00, 3.00 and 5.00 mg l-1) and α-naphthaleneacetic acid (NAA; 0.00, 0.10, 0.50, 1.00, 1.50 and 3.00 mg l-1). The BA alone at 0.50 or 2.00 mg l-1 was found to be best for shoot length, shoot number, node number, and leaf number than other concentrations of BA and BA in combination with NAA. The highest number of shoots (8.44) and the maximum average shoot length (13.16 cm) were recorded on MS medium supplemented with 0.50 mg l-1 BA without NAA. Root length (5.20 cm) and root number (8.77) were the maximum in the medium containing 0.10 mg l-1 NAA. The plantlets regenerated in vitro with well-developed shoots and roots were successfully established in pots containing peat and perlite and grown in a greenhouse within 4 weeks with a 100% survival rate. The regenerated plants were morphologically identical with donor plants and did not show any detectable phenotypic variation. Overall, BA at 0.50 or 2.00 mg l-1 had a better effect on shoot system than other concentrations of BA and BA in combination with NAA. NAA induced more root formation and root growth than BA.

چکیده انگلیسی:

برای تکثیر مطلوب گل اطلسی رقم ’مرلین مورن صورتی‘، ما یک روش ساده ریزازدیادی آزمایشگاهی ابداع کردیم. برای این منظور جوانه‌های انتهایی 30 روزه حاصل از گیاهان رشد یافته در محیط آزمایشگاهی از بذرهای هیبرید، در محیط موراشیگ و اسکوگ (MS) تیمار شده با 36 ترکیب تیماری حاصل از سطوح 6- بنزیل‌آمینوپورین (0، 5/0، 1، 2، 3 و 5 میلی گرم BA در لیتر) در سطوح آلفا نفتالین استیک اسید (0، 1/0، 5/0، 1، 5/1 و 3 میلی گرم NAA در لیتر) کشت داده شدند. نتایج نشان داد که فقط BA در مقدار 5/0 یا 2 میلی‌گرم در لیتر برای طول ساقه، تعداد ساقه، تعداد گره و تعداد برگ در مقایسه با سایر غلظت‌های BA و BA در ترکیب با NAA، بهتر بود. بیشترین تعداد شاخه فرعی (44/8) و حداکثر طول ساقه (16/13سانتیمتر) در محیط MS حاوی 50/0 میلی‌گرم در لیتر BA بدون NAA ثبت شد. حداکثر طول ریشه (2/5 سانتی متر) و تعداد ریشه (77/8) در محیط حاوی 10/0 میلی‌گرم NAA لیتر مشاهده شد. گیاهان پرورش یافته در محیط آزمایشگاهی در داخل گلدان‌های حاوی پرلیت و کوکوپیت کاشته شدند و در طی 4 هفته با بقای 100٪ رشد کردند. گیاهان تکثیر شده از لحاظ مورفولوژیکی با گیاهان مادری مشابه بودند و هیچ گونه تنوع فنوتیپی قابل تشخیص مشاهده نشد.

منابع و مأخذ:

Abu-Qaoud, H., Abu-Rayya, A. and Yaish, S. 2010. In vitro regeneration and somaclonal variation of Petunia hybrida. Journal of Fruit and Ornamental Plant Research, 18 (1): 71-81.

Aremu, A.O., Plačková, L., Pěnčík, A., Novák, O., Doležal, K. and van Staden, J. 2016. Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species. New Biotechnology, 33: 883–890.

Beyl, C.A. 2005. Getting started with tissue culture: Media preparation, sterile technique, and laboratory equipment. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. CRC Press, Florida, pp 19–38.

Clapa, D. and Cantor, M. 2006. Plant regeneration from stem cuttings of Petunia hybrida. Bulletin of University of Agricultural Science and Veteran Medicine, Cluj-napoca, Horticulture, 63 (1-2): 45-49.

Ċosiċ, T, Motyka, V., Raspor, M., Savić, J., Cingel. A., Vinterhalter, B., Vinterhalter, D., Trávníčková, A., Dobrev, P., Bohanec, B. and Ninković, S. 2015. In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): Effects of genotype, explant type and applied cytokinins. Plant Cell, Tissue and Organ Culture, 121: 741–760.

Ghafari-Esizad, S., Kaviani, B., Tarang, A.R. and Bohlooli-Zanjani, S. 2012. Micropropagation of lisianthus, an ornamental plant. Plant Omics Journal, 5: 314-319.

Jain, S.M. and Ochatt, S.J. 2010. Protocols for in vitro propagation of ornamental plants. Springer Protocols. Humana Press.

Kalimuthu, K., Senthilkumar, R. and Vijayakumar, S. 2007. In vitro micropropagation of orchid, Oncidium sp. (Dancing Dolls). African Journal of Biotechnology, 6 (10): 1171-74.

Kaviani, B. 2014. Micropropagation of ten weeks (Matthiola incana) and lisianthus (Eustoma grandiflorum) (two ornamental plants) by using kinetin (KIN), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid (2,4-D). Acta Scientiarum Polonorum-Hortorum Cultus, 13 (1): 141-154.

Kaviani, B. 2015. Some useful information about micropropagation. Journal of Ornamental Plant, 5 (1): 29-40.

Kaviani, B., Ahmadi Hesar, A., Tarang, A.R., Bohlooli Zanjani, S., Hashemabadi, D. and Ansari, M.H. 2013. Effect of kinetin (Kn) and naphthalene acetic acid (NAA) on the micropropagation of Matthiola incana using shoot tips, and callus induction and root formation on the leaf explants. African Journal of Agricultural Research, 8 (30): 4134-4149.

Kaviani, B., Ahmadi Hesar, A., Tarang, A.R., Bohloli Zanjani, S., Hashemabadi, D. and Rezaei, M.A. 2011. Callus induction and root formation on the leaf micro-cuttings of Matthiola incana using Kn and NAA. American-Eurasian Journal of Agricultural and Environmental Science, 11 (3): 456-461.

Kaviani, B. and Negahdar, N. 2017. Propagation, micropropagation and cryopreservation of Buxus hyrcana Pojark, an endangered ornamental shrub. South African Journal of Botany, 111: 326-335.‏

Kaviani, B., Zamiraei, F., Tarang, A.R., Bohlooli Zanjani, S. and Kaviani, B. 2014. In vitro flowerring and micropropagation of lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA). Acta Scientiarum Polonorum-Hortorum Cultus, 13 (4): 145-155.

Maberly, D.J. 1990. The Plant Book. A portable dictionary of the higher plants. Cambridge University Press, Cambridge, U.K.

Mneny, E.E. and Mantell, S.H. 2002. Clonal propagation of cashew (Anacardium occidentale L.) by tissue culture. Journal of Horticultural Science and Biotechnology, 77: 649–657.

Motte, H., Vereecke, D., Geelen, D. and Werbrouck, S. 2014. The molecular path to in vitro shoot regeneration. Biotechnological Advances, 32:107–121.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant, 15: 473-497.

Nazki, I.T., Wani, M.A., Din, A. and Slathia, D. 2018. In vitro propagation of ornamentals for maximizing livelihood security. In: Sustainable Agriculture Reviews, 27 (pp. 243-270). Springer, Cham.‏

Plačková, L., Hrdlička, J., Smýkalová, I., Cvečková, M., Novák, O., Griga, M. and Doležal, K. 2015. Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures. Plant Growth Regulators, 77:125–132.

Reuveni, M. and Evenor, D. 2007. On the effect of light on shoot regeneration in Petunia. Plant Cell, Tissue and Organ Culture, 89: 49-54.

Sujatha, G. and Ranjitha Kumari, B.D. 2007. Effect of phytohormones on micropropagation of Artemisia vulgaris L. Acta Physiology Plant, 29:189–195. 

van Staden, D., Zazimalora, E. and George, E.F. 2008. Plant growth regulators, II: Cytokinins, their analogues and inhibitors. In: Plant Propagation by Tissue Culture (edn. 3) (George E.F., et al., ed.), pp. 205-226, Springer.