تهيه نانوالياف پليمري زيست سازگار جديد حاوي عصاره گياه دافنه ماکروناتا و بررسي فعاليت ضدميکروبي آن
محورهای موضوعی : آلودگی های محیط زیست (آب، خاک و هوا)لاله صفوی نیا 1 , محمد رضا اخگر 2 * , بتول تهامی پور 3 , سید علی احمدی 4
1 - دانشجوي دکتراي شيمي آلي، گروه شيمی، واحد کرمان، دانشگاه ¬آزاد ¬اسلامي، کرمان، ايران.
2 - دانشيار شيمي¬ آلي، گروه شيمي، واحد کرمان، دانشگاه ¬آزاد ¬اسلامي، کرمان، ايران. *(مسوول مکاتبات)
3 - استاديار شيمي آلي، باشگاه پژوهشگران جوان و نخبگان، واحد سيرجان، دانشگاه ¬آزاد اسلامي، سيرجان، ايران
4 - استاديار شيمي¬ آلي، گروه شيمي، واحد کرمان، دانشگاه ¬آزاد ¬اسلامي، کرمان، ايران
کلید واژه: دافنه ماکروناتا , عصاره, پلي¬وينيل ¬الکل, نانوالياف, ضدميکروبي.,
چکیده مقاله :
زمینه و هدف: گياه دافنه ماکروناتا يکي از گونههاي جنس دافنه در ايران است که داراي خواص زيستي منحصر به فرد بوده و از ديرباز به‌عنوان يک گياه دارويي استفاده شده است. در اين مطالعه، نانوالياف پليمري زيستسازگار از پليوينيل الکل و عصاره گياه دافنه ماکروناتا به عنوان پوشش ضدميکروبي با کارآيي بالا به روش الکتروريسي تهيه و خواص ضدميکروبي آن بر روي باکتري های گرممثبت، گرممنفي و قارچ بررسي شد.
روش بررسی: در این تحقیق محلول پليمري با درصد وزني/وزني 12 درصد از پليمر پليوينيل الکل تهيه شد و 2 ميليليتر، از عصاره گياهي به 10 ميليليتر از محلول پليمري افزوده شد. به منظور تهيه نانوالياف، شرايط بهينه دستگاه الکتروريسي شامل ولتاژ 17 کيلوولت، نرخ تزريق محلول 3 ميليليتر بر ساعت، فاصله سر نازل تا جمعکننده 7 سانتي‌متر و سرعت جمعکننده 100 دور بر دقيقه تنظيم شد.
یافته ها: بعد از تهيه لايه پليمري، مورفولوژي و قطر نانوالياف به کمک تصوير ميکروسکوپ الکتروني روبشي معين شد و قطر متوسط نانوالياف تهيه شده در شرايط بهينه، برابر 80 نانومتر به‌دست آمد. بررسي فعاليت ضدميکروبي اين پوشش نشان داد که اين ساختار اثر ضدميکروبي قابلتوجهی بر روي باکتريها نشان ميدهد، به طوري که بيشترين اثر ضدميکروبي بر روي باکتري گرممثبت استرپتوکوکوس پیوژنز با قطر هاله عدم رشد 14 ميليمتر مشاهده شد.
بحث و نتیجه گیری: نانوپوشش حاصل در این پژوهش به دليل ساختار زيستسازگار، غيرسمي، ارزان قيمت و کارآيي بالا ميتواند به عنوان پوشش ضدميکروبي در حوزه زيستي و پزشکي جهت بستهبندي يا پوشش زخم مورد استفاده قرار گيرد.
Background & Objective: Daphne macronata is one of the species of Daphne genus in Iran that has unique biological properties and has been used as a medicinal plant for a long time. In this study, biocompatible polymer nanofibers from polyvinyl alcohol and Daphne macronata plant extract were prepared as an antimicrobial coating with high efficiency by electrospinning method and its antimicrobial properties on gram-positive, gram-negative and the fungus was examined.
Material and Methodology: In this study, a polymer solution with weight / weight of 12% polyvinyl alcohol polymer was prepared and 2 mL of plant extract was added to 10 mL of polymer solution. In order to prepare nanofibers, the optimal conditions of the electrospinning device including voltage of 17 kV, solution injection rate of 3 mL / h, nozzle head to collector distance of 7 cm and collector speed of 100 rpm were set.
Findings: After preparing the polymer layer, the morphology and diameter of the nanofibers were determined by scanning electron microscopy and the average diameter of the nanofibers prepared under optimal conditions was 80 nm. Examination of the antimicrobial activity of this coating showed that this structure shows a significant antimicrobial effect on bacteria, so that the highest antimicrobial effect was observed on the gram-positive bacterium Streptococcus pyogenes with a growth inhibition zone diameter of 14 mm.
Discussion and conclusion: The nanocoatings obtained in this study can be used as an antimicrobial coating in the biological and medical fields for packaging or wound dressing due to their biocompatible, non-toxic, inexpensive and high efficiency structure.
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