اثر تنش کم آبی بر بیان برخی ژن های مرتبط با کم آبی( MYB، AP2 ) و میکروRNAهای مرتبط در برگ های گیاه نرگس هلندی pseudonarcissus L. Narcissus
محورهای موضوعی :
زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
مریم السادات کامی شیرازی
1
,
گلناز تجدد
2
1 - گروه تکوین، دانشکده علوم وفناوریهای نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی ، تهران، ایران
2 - گروه زیست شناسی ،دانشکده علوم زیستی دانشگاه آزاد اسلامی ، واحد تهران شمال ،تهران، ایران
تاریخ دریافت : 1399/06/27
تاریخ پذیرش : 1399/10/15
تاریخ انتشار : 1402/09/01
کلید واژه:
تنش کم آبی,
miRNA159,
miRNA172,
MYB,
AP2,
چکیده مقاله :
پیشرفت هایی که در دنیا اتفاق می افتد، سبب ایجاد تغییراتی در شرایط زندگی موجودات زنده از جمله گیاهان می گردند. گیاهان باید بتوانند با روش های مختلف حتی روش های مولکولی با شرایط تنش زای محیطی سازگارشوند. یکی از ساز و کارها، تنظیم بیان ژن ها بعد از رونویسی توسط miRNAs (میکروRNAها) است. میکروRNA ها، اغلب 20 تا 22 نوکلئوتید دارند که برخی از ژن های هدف آنها متعلق به عوامل رونویسی می باشند. بیان میکروRNA ها در پاسخ به تنش کم آبی تغییر می کند. در پژوهش حاضر، پیازهای نرگس هلندی در شرایط متفاوت آبیاری (از یک بار در هفته تا دو ماه یک بار) کشت شدند. از برگ‌های گیاهان 60 روزه برای استخراج ژن هدف میکروRNA 159 ، MYB و ژن هدف میکروRNA 172، AP2 ، با روش qPCR استفاده شد. نتایج نشان دهنده افزایش بیان معنی دار ژنهای MYB و AP2 و عدم بیان میکروRNA های 159 و 172در نمونه های تیمار و کنترل بود. لذا، miR172-miR159 تحت تاثیر تنش کم آبی قرار نمی گیرد. با توجه به اینکه بیان ژنهای کم آبی برای اولین بار در گیاه نرگس هلندی ارزیابی شده است، به همین دلیل این پژوهش می تواند زمینه خوبی برای بررسی بیشتر در این مورد را فراهم کند.
چکیده انگلیسی:
Advances in the world are causing changes in the living conditions of living things, including plants. Plants must be able to adapt to environmental stressful conditions in a variety of ways, even molecular methods. One mechanism is to regulate gene expression after transcription by miRNAs (microRNAs). MicroRNAs often have 20 to 22 nucleotides, some of whose target genes belong to transcription factors. The expression of microRNAs changes in response to dehydration stress. In the present study, Narcissus bulbs were grown under different irrigation conditions (from once a week to once every two months). The leaves of 60-day-old plants were used to extract the target gene of microRNA 159, MYB and the target gene of microRNA 172, AP2, by qPCR method. The results showed a significant increase in the expression of MYB and AP2 genes and no expression of 159 and 172 microRNAs in the treated and control samples. Therefore, miR172-miR159 is not affected by dehydration stress. Given that the expression of dehydration genes has been evaluated for the first time in the Narcissus pseudonarcissus, therefore, this study can provide a good basis for further investigation in this case.
منابع و مأخذ:
Singh SP, Upadhyay SK, Pandey A, Kumar S. Molecular approaches in plant biology and environmental challenges. Springer Singapore; 2019.
Bhatia G, Singh A, Verma D, Sharma S, Singh K. Genome-wide investigation of regulatory roles of lncRNAs in response to heat and drought stress in Brassica juncea (Indian mustard). Environmental and Experimental Botany. 2020 Mar 1;171:103922.
Fei X, Li J, Kong L, Hu H, Tian J, Liu Y, Wei A. miRNAs and their target genes regulate the antioxidant system of Zanthoxylum bungeanum under drought stress. Plant Physiology and Biochemistry. 2020 May 1;150:196-203.
Fei X, Li J, Kong L, Hu H, Tian J, Liu Y, Wei A. miRNAs and their target genes regulate the antioxidant system of Zanthoxylum bungeanum under drought stress. Plant Physiology and Biochemistry. 2020 May 1;150:196-203.
Liu J, Wang H, Chua NH. Long noncoding RNA transcriptome of plants. Plant biotechnology journal. 2015 Apr;13(3):319-28.
Khraiwesh B, Zhu JK, Zhu J. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms. 2012 Feb 1;1819(2):137-48.
Willmann MR, Poethig RS. Conservation and evolution of miRNA regulatory programs in plant development. Current opinion in plant biology. 2007 Oct 1;10(5):503-11.
Jiang D, Zhou L, Chen W, Ye N, Xia J, Zhuang C. Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways. Rice. 2019 Dec;12:1-1.
Zhou M, Tang W. MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells. Molecular Genetics and Genomics. 2019 Apr 5;294:379-93.
Liu HH, Tian X, Li YJ, Wu CA, Zheng CC. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. Rna. 2008 May 1;14(5):836-43.
Wei L, Zhang D, Xiang F, Zhang Z. Differentially expressed miRNAs potentially involved in the regulation of defense mechanism to drought stress in maize seedlings. International Journal of Plant Sciences. 2009 Oct;170(8):979-89.
Yang J, Zhang N, Mi X, Wu L, Ma R, Zhu X, Yao L, Jin X, Si H, Wang D. Identification of miR159s and their target genes and expression analysis under drought stress in potato. Computational biology and chemistry. 2014 Dec 1;53:204-13.
Hackenberg M, Gustafson P, Langridge P, Shi BJ. Differential expression of micro RNA s and other small RNA s in barley between water and drought conditions. Plant biotechnology journal. 2015 Jan;13(1):2-13.
Li Y, Wan L, Bi S, Wan X, Li Z, Cao J, Tong Z, Xu H, He F, Li X. Identification of drought-responsive microRNAs from roots and leaves of alfalfa by high-throughput sequencing. Genes. 2017 Apr 13;8(4):119.
Samad AF, Sajad M, Nazaruddin N, Fauzi IA, Murad AM, Zainal Z, Ismail I. MicroRNA and transcription factor: key players in plant regulatory network.
Frontiers in plant science. 2017 Apr 12;8:565.
Agarwal PK, Jha B. Transcription factors in plants and ABA dependent and independent abiotic stress signalling. Biologia Plantarum. 2010 Jun;54(2):201-12.
Luscombe NM, Thornton JM. Protein–DNA interactions: amino acid conservation and the effects of mutations on binding specificity. Journal of molecular biology. 2002 Jul 26;320(5):991-1009.
Chiu TP, Xin B, Markarian N, Wang Y, Rohs R. TFBSshape: an expanded motif database for DNA shape features of transcription factor binding sites. Nucleic acids research. 2020 Jan 8;48(D1):D246-55.
Lata C, Prasad M. Role of DREBs in regulation of abiotic stress responses in plants. Journal of experimental botany. 2011 Oct 1;62(14):4731-48.
Wang J, Wang F, Jin C, Tong Y, Wang T. A R2R3-MYB transcription factor VvMYBF1 from grapevine (Vitis vinifera L.) regulates flavonoids accumulation and abiotic stress tolerance in transgenic Arabidopsis. The Journal of Horticultural Science and Biotechnology. 2020 Mar 3;95(2):147-61.
Liu X, Meng P, Yang G, Zhang M, Peng S, Zhai MZ. Genome-wide identification and transcript profiles of walnut heat stress transcription factor involved in abiotic stress. BMC genomics. 2020 Dec;21:1-3.
Brasileiro AC, Morgante CV, Araujo AC, Leal-Bertioli SC, Silva AK, Martins AC, Vinson CC, Santos CM, Bonfim O, Togawa RC, Saraiva MA. Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant molecular biology reporter. 2015 Dec;33:1876-92.
Moumeni A, Satoh K, Kondoh H, Asano T, Hosaka A, Venuprasad R, Serraj R, Kumar A, Leung H, Kikuchi S. Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress. BMC plant biology. 2011 Dec;11:1-7.
Berry-Lowe SL, Mc Knight TD, Shah DM, Meagher RB. The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1, 5-bisphosphate carboxylase in soybean. Journal of molecular and applied genetics. 1982 Jan 1;1(6):483-98.
Nakashima K, Ito Y, Yamaguchi-Shinozaki K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant physiology. 2009 Jan;149(1):88-95.
Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends in plant science. 2010 Oct 1;15(10):573-81.
Miyakawa T, Fujita Y, Yamaguchi-Shinozaki K, Tanokura M. Structure and function of abscisic acid receptors. Trends in plant science. 2013 May 1;18(5):259-66.
Xu ZS, Chen M, Li LC, Ma YZ. Functions and application of the AP2/ERF transcription factor family in crop improvement F. Journal of integrative plant biology. 2011 Jul;53(7):570-85.
Zhang B, Pan X, Cannon CH, Cobb GP, Anderson TA. Conservation and divergence of plant microRNA genes. The Plant Journal. 2006 Apr;46(2):243-59.
Trindade I, Capitão C, Dalmay T, Fevereiro MP, Santos DM. miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula. Planta. 2010 Feb;231:705-16.
Wu P, Han S, Zhao W, Chen T, Zhou J, Li L. Genome-wide identification of abiotic stress-regulated and novel microRNAs in mulberry leaf. Plant Physiology and Biochemistry. 2015 Oct 1;95:75-82.
Lu S, Sun YH, Shi R, Clark C, Li L, Chiang VL. Novel and mechanical stress–responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis. The Plant Cell. 2005 Aug;17(8):2186-203.
Chen H, Li Z, Xiong L. A plant microRNA regulates the adaptation of roots to drought stress. Febs Letters. 2012 Jun 12;586(12):1742-7.
Xu D, Duan X, Wang B, Hong B, Ho TH, Wu R. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant physiology. 1996 Jan;110(1):249-57.
_||_
Singh SP, Upadhyay SK, Pandey A, Kumar S. Molecular approaches in plant biology and environmental challenges. Springer Singapore; 2019.
Bhatia G, Singh A, Verma D, Sharma S, Singh K. Genome-wide investigation of regulatory roles of lncRNAs in response to heat and drought stress in Brassica juncea (Indian mustard). Environmental and Experimental Botany. 2020 Mar 1;171:103922.
Fei X, Li J, Kong L, Hu H, Tian J, Liu Y, Wei A. miRNAs and their target genes regulate the antioxidant system of Zanthoxylum bungeanum under drought stress. Plant Physiology and Biochemistry. 2020 May 1;150:196-203.
Fei X, Li J, Kong L, Hu H, Tian J, Liu Y, Wei A. miRNAs and their target genes regulate the antioxidant system of Zanthoxylum bungeanum under drought stress. Plant Physiology and Biochemistry. 2020 May 1;150:196-203.
Liu J, Wang H, Chua NH. Long noncoding RNA transcriptome of plants. Plant biotechnology journal. 2015 Apr;13(3):319-28.
Khraiwesh B, Zhu JK, Zhu J. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms. 2012 Feb 1;1819(2):137-48.
Willmann MR, Poethig RS. Conservation and evolution of miRNA regulatory programs in plant development. Current opinion in plant biology. 2007 Oct 1;10(5):503-11.
Jiang D, Zhou L, Chen W, Ye N, Xia J, Zhuang C. Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways. Rice. 2019 Dec;12:1-1.
Zhou M, Tang W. MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells. Molecular Genetics and Genomics. 2019 Apr 5;294:379-93.
Liu HH, Tian X, Li YJ, Wu CA, Zheng CC. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. Rna. 2008 May 1;14(5):836-43.
Wei L, Zhang D, Xiang F, Zhang Z. Differentially expressed miRNAs potentially involved in the regulation of defense mechanism to drought stress in maize seedlings. International Journal of Plant Sciences. 2009 Oct;170(8):979-89.
Yang J, Zhang N, Mi X, Wu L, Ma R, Zhu X, Yao L, Jin X, Si H, Wang D. Identification of miR159s and their target genes and expression analysis under drought stress in potato. Computational biology and chemistry. 2014 Dec 1;53:204-13.
Hackenberg M, Gustafson P, Langridge P, Shi BJ. Differential expression of micro RNA s and other small RNA s in barley between water and drought conditions. Plant biotechnology journal. 2015 Jan;13(1):2-13.
Li Y, Wan L, Bi S, Wan X, Li Z, Cao J, Tong Z, Xu H, He F, Li X. Identification of drought-responsive microRNAs from roots and leaves of alfalfa by high-throughput sequencing. Genes. 2017 Apr 13;8(4):119.
Samad AF, Sajad M, Nazaruddin N, Fauzi IA, Murad AM, Zainal Z, Ismail I. MicroRNA and transcription factor: key players in plant regulatory network.
Frontiers in plant science. 2017 Apr 12;8:565.
Agarwal PK, Jha B. Transcription factors in plants and ABA dependent and independent abiotic stress signalling. Biologia Plantarum. 2010 Jun;54(2):201-12.
Luscombe NM, Thornton JM. Protein–DNA interactions: amino acid conservation and the effects of mutations on binding specificity. Journal of molecular biology. 2002 Jul 26;320(5):991-1009.
Chiu TP, Xin B, Markarian N, Wang Y, Rohs R. TFBSshape: an expanded motif database for DNA shape features of transcription factor binding sites. Nucleic acids research. 2020 Jan 8;48(D1):D246-55.
Lata C, Prasad M. Role of DREBs in regulation of abiotic stress responses in plants. Journal of experimental botany. 2011 Oct 1;62(14):4731-48.
Wang J, Wang F, Jin C, Tong Y, Wang T. A R2R3-MYB transcription factor VvMYBF1 from grapevine (Vitis vinifera L.) regulates flavonoids accumulation and abiotic stress tolerance in transgenic Arabidopsis. The Journal of Horticultural Science and Biotechnology. 2020 Mar 3;95(2):147-61.
Liu X, Meng P, Yang G, Zhang M, Peng S, Zhai MZ. Genome-wide identification and transcript profiles of walnut heat stress transcription factor involved in abiotic stress. BMC genomics. 2020 Dec;21:1-3.
Brasileiro AC, Morgante CV, Araujo AC, Leal-Bertioli SC, Silva AK, Martins AC, Vinson CC, Santos CM, Bonfim O, Togawa RC, Saraiva MA. Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant molecular biology reporter. 2015 Dec;33:1876-92.
Moumeni A, Satoh K, Kondoh H, Asano T, Hosaka A, Venuprasad R, Serraj R, Kumar A, Leung H, Kikuchi S. Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress. BMC plant biology. 2011 Dec;11:1-7.
Berry-Lowe SL, Mc Knight TD, Shah DM, Meagher RB. The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1, 5-bisphosphate carboxylase in soybean. Journal of molecular and applied genetics. 1982 Jan 1;1(6):483-98.
Nakashima K, Ito Y, Yamaguchi-Shinozaki K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant physiology. 2009 Jan;149(1):88-95.
Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends in plant science. 2010 Oct 1;15(10):573-81.
Miyakawa T, Fujita Y, Yamaguchi-Shinozaki K, Tanokura M. Structure and function of abscisic acid receptors. Trends in plant science. 2013 May 1;18(5):259-66.
Xu ZS, Chen M, Li LC, Ma YZ. Functions and application of the AP2/ERF transcription factor family in crop improvement F. Journal of integrative plant biology. 2011 Jul;53(7):570-85.
Zhang B, Pan X, Cannon CH, Cobb GP, Anderson TA. Conservation and divergence of plant microRNA genes. The Plant Journal. 2006 Apr;46(2):243-59.
Trindade I, Capitão C, Dalmay T, Fevereiro MP, Santos DM. miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula. Planta. 2010 Feb;231:705-16.
Wu P, Han S, Zhao W, Chen T, Zhou J, Li L. Genome-wide identification of abiotic stress-regulated and novel microRNAs in mulberry leaf. Plant Physiology and Biochemistry. 2015 Oct 1;95:75-82.
Lu S, Sun YH, Shi R, Clark C, Li L, Chiang VL. Novel and mechanical stress–responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis. The Plant Cell. 2005 Aug;17(8):2186-203.
Chen H, Li Z, Xiong L. A plant microRNA regulates the adaptation of roots to drought stress. Febs Letters. 2012 Jun 12;586(12):1742-7.
Xu D, Duan X, Wang B, Hong B, Ho TH, Wu R. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant physiology. 1996 Jan;110(1):249-57.
