ارزیابی خاصیت ضد قارچی عصاره های میوه آستراگالوس کرناتوس و صمغ ترنجبین ایرانی بر روی گونه های کاندیدیایی جدا شده از شیر گاوهای مبتلا به ورم پستان تحت بالینی
محورهای موضوعی : پاتوبیولوژی مقایسه ای
منصوره پاک نژادی
1
(دکترای تخصصی قارچ شناسی، استادیار گروه میکروبیولوژی، دانشکده علوم پایه دانشگاه ازاد اسلامی واحد شهر قدس)
بهاره همت یار
2
(گروه میکروبیولوژی، دانشکده علوم پایه، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران)
حمیرا سیده سلیمانی
3
(گروه میکروبیولوژی، دانشکده علوم پایه، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران)
کلید واژه: ورم پستان تحت بالینی, گونه های کاندیدا, صمغ ترنجبین, میوه آستراگالوس کرناتوس, PCR-RFLP,
چکیده مقاله :
تشخیص و درمان مناسب ورم پستان تحت بالینی کاندیدیایی می تواند تأثیر بسزائی در سلامت گاوهای شیری و محصولات لبنی داشته باشد. اما عوارض جانبی داروهای ضد قارچی، به عنوان اصلی ترین محدودیت درمانی در حوزه دامپزشکی شناخته شده است. لذا هدف از مطالعه حاضر، شناسایی مولکولی گونه های کاندیدیایی در شیر گاوهای مبتلا به عفونت تحت بالینی و ارزیابی اثر ضد قارچی عصاره میوه آستراگالوس کرناتوس و صمغ گیاه خارشتر (ترنجبین) ایرانی می باشد. در این مطالعه از 70 شیر خام، 32 نمونه براساس افزایش تعداد سلولهای سوماتیک انتخاب شدند. سپس شناسایی اولیه و قطعی گونه های کاندیدا به ترتیب با استفاده از محیط کروموژنیک و PCR-RFLP انجام شد. درنهایت، اثر عصاره های اتانولی و متانولی (غلظت 1000، 500 و 250 mg/ml) هر دو گیاه، توسط روشهای چاهک و دیسک گذاری ارزیابی شدند. یافته ها نشان داد که از 15 نمونه مثبت، گونه های غیرآلبیکنس شامل کاندیدا کفیر، کاندیدا فاماتا و مخلوط آنها، فراوانی بیشتری (33/93%) نسبت به کاندیدا آلبیکنس (67/6%) داشتند. براساس سنجش خاصیت ضد قارچی، میانگین اثر مهاری نیستاتین بر کاندیدا فاماتا (6/28 میلیمتر)، آلبیکنس (7/26 میلیمتر) و کفیر (8/25 میلیمتر) نشان دهنده حساسیت هر سه گونه بدون تفاوت معنا دار (171/0= P-value) بود. درحالیکه، غلظتهای تهیه شده از هر دو عصاره، دارای اثر قابل توجهی در مقایسه با نیستاتین نبودند. با توجه به تنوع عوامل دخیل در کیفیت و میزان اثر ترکیبات گیاهی بر پاتوژنهای حیوانی، شناسایی گسترده تر بر روی فاکتورهای محیطی و ترکیبات ضد قارچی مستخرج از میوه آستراگالوس کرناتوس و صمغ ترنجبین ایرانی ضروری می باشد.
Appropriate diagnosis and treatment of subclinical mastitis caused by Candida species can have a great impact on the health of dairy cows and dairy products. However, the side effects of antifungal drugs are known as one of the main therapeutic limitations in the veterinary medicine. Therefore, the present study aims to identify Candida species in the milk of cows with subclinical mastitis using molecular methods. In addition, to investigate the antifungal properties of Persian Taranjebin manna and Astragalus crenatus fruit extracts. In this research, out of 70 raw milk, 32 samples were selected based on the excessive number of somatic cells. Primary and definitive differentiation of Candida species were conducted using chromogenic medium and PCR-RFLP, respectively. Finally, disk and well diffusion agar methods were applied to identify the effect of ethanolic and methanolic extracts at 1000, 500, and 250 mg/ml concentrations. The findings indicated that out of 15 positive samples, non-albicans species including C. kefir, C. famata, and their mixture had a higher frequency (99.33%) than C. albicans (6.67%). According to the antifungal properties measurement, the average inhibitory effect of nystatin on C. famata (28.6 mm), C. albicans (26.7 mm), and C. kefir (25.8 mm) showed the sensitivity of all three species without significant difference (P-value=0.171). However, the concentrations prepared from the both extracts did not have a significant effect compared to nystatin. Considering the variety of factors involved in the quality and effect of herbal compounds on animal pathogens, it is necessary to identify more extensive environmental factors and antifungal compounds extracted from Persian Taranjebin manna and A. crenatus fruit.
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