• صفحه اصلی
  • بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

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آدرس مقاله


کد مقاله : EJMP-1702-1192 (R1) بازدید : 246 صفحه: 49 - 68

20.1001.1.23223235.1396.5.2.5.6

نوع مقاله: پژوهشی

بررسی فیتوشیمیایی، ارزیابی عملکرد آنتی‌اکسیدانی و آنتی-باکتریایی عصاره‌های پنج گونه دارویی Pulicaria gnaphalodes (Vent.) Boiss، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L.، Hibiscus sabdariffa L. در استان سیستان و بلوچستان

محورهای موضوعی : فیتوشیمی

امید عزیزیان شرمه 1 , محرم ولی زاده 2 , علی قاسمی 3 , احمد مهربان 4 , افسانه کمالی دلجو 5

1 - دانش‌آموخته کارشناسی ارشد فیتوشیمی، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران
2 - استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران
3 - مربی، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
4 - استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد زاهدان، زاهدان، ایران
5 - دانش آموخته کارشناسی ارشد زراعت و اصلاح نباتات، باشگاه پژوهشگران جوان و نخبگان، واحد زاهدان، دانشگاه آزاد اسلامی، زاهدان، ایران

تاریخ دریافت : 1395/11/15 تاریخ پذیرش : 1396/01/19 تاریخ انتشار : 1396/06/01

کلید واژه: سیستان و بلوچستان, آنتی‌اکسیدانی, فلاونوئید, فنول, آنتی‌باکتریایی, گیاهان دارویی بومی,

چکیده مقاله :

مطالعه حاضر به بررسی ترکیبات فیتوشیمیایی فنول و فلاونوئید کل، ارزیابی عملکرد آنتی اکسیدانی و آنتی باکتریایی سه عصاره متانولی، اتانولی و آبی پنج گونه مختلف گیاهان دارویی Pulicaria gnaphalodes (Vent.) Boiss.، Rhazya stricta L.، Salvadora persica L.، Teucrium polium L. و Hibiscus sabdariffa L. در منطقه سیستان و بلوچستان می پردازد. پس از جمع آوری گیاهان و تهیه عصاره ها به روش ماسراسیون، محتوای فنولی و فلاونوئیدی به‌ترتیب با روش های معرف فولین-سیوکالتیو و رنگ سنجی کلرید آلومینیوم، فعالیت آنتی اکسیدانی با دو روش DPPH و FRAP و فعالیت آنتی باکتریایی علیه باکتری های استافیلوکوکوس اورئوس، انتروکوکوس فکالیس، اشرشیاکلی و سالمونلا تیفیموریوم به روش دیسک دیفیوژن (انتشار دیسک) سنجیده شده است. نتایج نشان دادند که در تمامی گونه ها، عصاره متانولی دارای بیشترین ترکیبات ثانویه و فعالیت های آنتی‌اکسیدانی و آنتی باکتریایی بوده است. عصاره متانولی گونه Hibiscus sabdariffa L. دارای بیشترین میزان ترکیبات فنولی (mgGAE/g44/6±54/32) و فلاونوئیدی (mgGAE/g00/1±11/27) و فعالیت آنتی اکسیدانی (µg/ml21/1±34/17IC50= و mMFe2+/mg27/2±19/55) و فعالیت آنتی باکتریایی علیه باکتری استافیلوکوکوس اورئوس با قطر‌هاله عدم رشد 00/1±27 میلی متر بوده است. در مقابل عصاره آبی گونه Teucrium poliumL.دارای کمترین میزان ترکیبات فنولی (mgGAE/g11/1±33/4) و فلاونوئیدی (mgGAE/g08/0±03/2) و فعالیت آنتی‌اکسیدانی (µg/ml02/2±71/220 IC50= و mM Fe2+/mg sample99/0±76/8) و فعالیت آنتی باکتریایی علیه باکتری انترکوکوس فکالیس با قطر‌هاله عدم رشد 50/1±4 میلی متر و اشرشیاکلی با قطر‌ هاله عدم رشد 57/0±4 میلی متر بوده است. به‌طورکلی بر اساس نتایج بدست آمده، گیاهان مورد مطالعه می توانند کاندیدای خوبی جهت درمان بیماری های ناشی از استرس‌های اکسیداتیوی و بیماری های ناشی از میکروب های بیماری زا قرار گیرند.

چکیده انگلیسی:

This present study is carried out to evaluate the phytochemical compounds such as total phenolic and total flavonoid contents, antioxidant and antibacterial activities of three extracts (methanolic, ethanolic and aqueous) in five different species of medicinal plants: Pulicaria gnaphalodes (Vent.)  Boiss.,  Rhazya stricta L., Salvadora persica L., Teucrium polium L., and Hibiscus sabdariffa L.. These samples were collected in different regions from Sistan and Baluchestan, 2014.The plant extracts were obtained by maceration method, phenolic and flavonoid contents were determined by Folin-Ciocaltiu and Aluminum Chloride colorimetric, antioxidant activities were studied by two methods (DPPH and FRAP) and then antimicrobial activities were estimated by Disk-Diffusion method against four bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Salmonella typhimurium. The results were showed that in all species, the methanolic extract had high amount of secondary compounds, antioxidant and antibacterial activities. The methanolic extract of Hibiscus sabdariffa L. had maximum total phenolic (32.54±6.44 mgGAE/g) , flavonoid contents(27.11±1.00mgQUE/g) and antioxidant activity, (IC50=17.34±1.21µg/ml, 55.19±2.27 mM Fe2+/mg) and antibacterial activity against Staphylococcus aureus with inhibition zone (27±1.00mm) was the most sensitive bacteria to plant extracts. In contrast, the aqueous extract of Teucrium polium L. had the minimum value of total phenolic , flavonoid contents , antioxidant activity and antibacterial activity .Overall, these plants could be a good candidate for the treatment of diseases caused by oxidative stress and pathogenic microbes.

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