بررسی اثرات تحریکی و مهاری نیتریک اکساید بر تعداد سلول‌های کوپفر و حجم سینوزوئیدهای کبد موش صحرایی
محورهای موضوعی : فصلنامه زیست شناسی جانوری
محمد تلواری
1
,
عطاردالسادات مصطفوی نیا
2
,
پریوش داودی
3
,
مبینا زرگر
4
,
سید محمد حسین نوری موگهی
5
*
1 - گروه علوم تشریحی و اعصاب شناختی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران، تهران، ایران.
2 - گروه علوم تشریحی و اعصاب شناختی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران، تهران، ایران
3 - گروه علوم تشریحی و اعصاب شناختی، دانشگاه آزاد اسلامی واحد پزشکی تهران، تهران، ایران
4 - گروه علوم تشریحی و اعصاب شناختی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران، تهران، ایران.
5 - گروه علوم تشریحی، دانشکده پزشکی، دانشگاه آزاد اسلامی اهواز، اهواز، ایران.
کلید واژه: کبد, سلول کوپفر, سینوزوئید, نیتریک اکساید, L-Arginine, L-NAME,
چکیده مقاله :
نیتریک اکساید(NO) یک مولکول دو اتمی و چربی دوست است که در بسیاری از اندام‌های بدن توسط آنزیم نیتریک اکساید سنتاز(Nos) از L-Arginine تولید می‌شود. هدف از این مطالعه و بررسی اثرات تحریکی و مهاری نیتریک اکساید بر تعداد سلول‌های کوپفر و حجم سینوزوئیدهای کبد در موش صحرایی است. 40 سر موش صحرایی ماده بالغ باردار نژاد ویستار(Wistar) با وزن متوسط 200-250 گرم به طور تصادفی در 5 گروه قرار گرفتند. به جز گروه کنترل، بقیه گروه‌ها به ترتیب به ازای هر کیلوگرم وزن حیوان، 200 میلی گرم از پودر L-Arginine و 20 میلی گرم از پودر L-NAME حل شده در محلول نرمال سالین، نرمال سالین و مخلوط دو ماده L-NAME و L-Arginine را با همان دوزهای مشابه در روزهای سوم، چهارم و پنجم حاملگی به صورت داخل صفاقی دریافت کردند. سپس موش‌ها را در روز 18 حاملگی، بیهوش کرده و کبد حیوانات را خارج و تعداد سلول‌های کوپفر و حجم سینوزوییدها را با رنگ آمیزی هماتوکسیلین ائوزین(H&E) بررسی شد. در این بررسی، تعداد سلول‌های کوپفر و حجم سینوزوئیدها در گروه‌های L-Arginine و L-NAME، تفاوت معنی‌داری با گروه کنترل داشت، در حالی که تفاوت معنی‌داری در مقایسه دو متغیر فوق بین گروه L-Arginine+L-NAME و گروه کنترل مشاهده نشد. این مطالعه نشان داد که مصرف ترکیبی از دو ماده L-NAME و L-Arginine می‌تواند اثرات تحریکی و مهاری نیتریک اکساید بر تعداد سلول‌های کوپفر و حجم سینوزوئیدهای کبد را تعدیل کند
Nitric oxide (NO) is a diatomic and lipophilic molecule that is produced by the nitric oxide synthase (Nos) from L-Arginine in many organs of the body. Considering the important roles of nitric oxide in many physiological processes of the body and limited number of researches that have been done in the field of its effects on Kupffer cells and volume of sinusoids, the purpose of this study is to evaluate excitatory and inhibitory effects of Nitric Oxide on liver histology in rat. Forty female Wistar rats with average weight of 200-250 gr after pregnancy were randomly divided into 5 groups. Except the control group, the rest of the groups intraperitoneally received Normal Saline, L-Arginine, L-NAME and mixture of L-NAME and L-Arginine respectively with the same dose in the 3th, 4th and 5th days of pregnancy. Then, in 18th day of pregnancy, Rats were anesthetized and we took out the animal’s liver and examined Kupffer cells and volume of sinusoids with Hematoxylin-Eosin staining. In this study, Kupffer cells and volume of sinusoids were significantly different in L-NAME and L-Arginine groups with control group, whereas in L-NAME+Arginine group no significant difference was observed with the control group. This study showed that the combined use of L-Arginine and L-NAME can modulate the excitatory and inhibitory effects of nitric oxide on the rat liver.
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