تهیه داربست‌های سلول زدایی شده ی رگ گاو و ارزیابی مدل‌های هایپرالاستیک به منظور کاربرد در مهندسی بافت عروق
محورهای موضوعی : فصلنامه زیست شناسی جانوری
مهرداد شیخلو
1
*
,
آرش عبدالمالکی
2
,
عباس صباحی نمینی
3
1 - گروه علوم مهندسی، دانشکده فناوری های نوین، دانشگاه محقق اردبیلی، نمین، ایران
2 - Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
3 - گروه علوم مهندسی، دانشکده فناوری های نوین، دانشگاه محقق اردبیلی، نمین، ایران
کلید واژه: خصوصیات مکانیکی, هایپرالاستیک, مهندسی بافت قلبی عروقی, پزشکی ترمیمی, داربست,
چکیده مقاله :
بیماری عروق کرونر مسئول تقریباً 30 درصد از کل مرگ و میرها در سراسر جهان است. هدف از این پژوهش تهیه داربست سلول‌زدایی شده رگ گاو و مقایسه آن با نمونه کنترل و همچنین ارزیابی رفتار مکانیکی آن بود. مدل‌سازی و انتخاب صحیح معادلات ساختاری برای تحلیل رفتار مکانیکی بافت‌ها از اهمیت حیاتی برخوردار است. استفاده از مدل‌های ساختاری هایپرالاستیک برای پیش بینی رفتار مکانیکی غیر خطی بافت‌های نرم رایج است، با این حال، مدل‌های هایپرالاستیک به مجموعه‌ای از ثابتهای مواد بستگی دارند که باید به صورت آزمایشگاهی محاسبه شوند. در این مطالعه از یک روش محاسباتی/آزمایشگاهی برای مطالعه رفتار مکانیکی غیرخطی بافت‌های رگ و داربست تحت کشش تک محوری استفاده شد. ثابتهای ماده برای سه مدل مختلف مواد هایپرالاستیک از طریق روش‌های معکوس محاسبه شد. جستجو برای یک مقدار بهینه برای هر مجموعه از پارامترهای مواد با استفاده از روش مینیمم کردن مجموع مربعات خطا انجام شد. دقت برازش رابطه تنش- نسبت کشش تئوری با نتایج آزمایشگاهی ارزیابی شد. مشاهده شد که بافت رگ در مقایسه با داربست در مقابل کشش مقاومت بیشتری از خود نشان می دهد، خواص مکانیکی بالاتر رگ به خاطر الاستین و محتوای کلاژن موجود در دیواره رگ می‌باشد. برای رگ مدل‌های یئو و آگدن به خوبی با نتایج آزمایشگاهی برازش کردند ولی برای داربست بهترین نتایج با مدل یئو به دست آمد. تمام مدل‌های مواد بررسی شده دقت کمتری در ناحیه کشش‌های کوچک نشان دادند. مشاهده شد که برای مدل‌سازی رفتار مکانیکی رگ و داربست به سه پارامتر ماده و در برخی موارد دو پارامتر ماده نیاز است. به طور کلی نتایج نشان می‌دهد که داربست‌های حاصل از سلول‌زدایی با توجه به حفظ ترکیبات اصلی بافت مورد نظر و همچنین مقاومت مکانیکی مناسب، مدلی ایده‌آل برای کاربردهای مهندسی بافت عروق می‌باشد.
Coronary artery disease (CAD) is responsible for approximately 30% of deaths worldwide. The purpose of this research was to prepare a decellularized bovine vein scaffold and compare it with the control sample and evaluate its mechanical behavior. Modeling and selection of structural equations is of vital importance for analyzing the mechanical behavior of tissues. It is common to use hyperelastic structural models to predict the nonlinear behavior of soft tissues, however, hyperelastic models depend on a set of material constants that must be calculated experimentally. In this study, a computational/laboratory method was used to study the nonlinear mechanical behavior of vessel and scaffold tissues under uniaxial tension. Material constants were calculated for three different hyperelastic material models through inverse methods. The search for an optimal value for each set of material constants was performed using the sum of squared error minimization method. The accuracy of the fitted theoretical stress-stretch ratio relationship was evaluated with the experimental results. It was observed that the tissue of the vessel shows more resistance to tension compared to the scaffold; the higher mechanical properties of the vessel are due to the elastin and collagen content in the vessel wall. For the vessel, the Yeo and Ogden models fit well with the laboratory results, but for the scaffold, the best results were obtained with the Yeo model. All of the investigated material models showed less accuracy in the area of small tension ratios. It was observed that three material parameters and in some cases two material parameters are needed to model the mechanical behavior of vessels and scaffolds. Overall, the results show that scaffolds obtained from decellularization are an ideal model for vascular tissue engineering applications, considering the preservation of the main components of the desired tissue as well as appropriate mechanical strength.
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