تاثير مصرف جيره هاي مكمل شده با سطوح مختلف گوانيدينواستيك اسيد بر صفات لاشه، برخي فراسنجه‌هاي بيوشيميايي خون، فعاليت آنزیمهاي كبدي، جمعيت ميكروبي و ريخت شناسي روده در بلدرچین‌هاي گوشتي ژاپني
محورهای موضوعی : فصلنامه زیست شناسی جانوریرضا علي پور فيل آبادي 1 , مصطفی فغانی 2 * , فرشید خیری 3
1 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
کلید واژه: گوانيدينو استيك اسيد, فراسنجه‌هاي بيوشيمايي خون, آنزیم كبدي, ريخت شناسي روده, بلدرچين ژاپني,
چکیده مقاله :
به منظور بررسي اثر مصرف جيرههاي مكمل شده با سطوح مختلف گوانيدينواستيكاسید بر عملكرد، برخي فراسنجه‌هاي بيوشيميايي خون، جمعيت ميكروبي و ريختشناسي روده در بلدرچین‌هاي گوشتي ژاپني، تعداد 288 قطعه بلدرچین ژاپنی در سن 1-35 روزگی با 4 تیمار آزمایشی شامل گروه شاهد و مصرف کننده گوانيدينو استيك اسيد با سطوح 5/0 و 1 و 5/1 گرم/کیلوگرم از خوراک استاندارد با 6 تکرار و 12 قطعه بلدرچین تخمگذار در هر تکرار استقاده گرديد. در انتهاي دوره (35 روزگي) پس از كشتار دو قطعه پرنده از هر تكرار، صفات لاشه بلدرچین‌ها، فراسنجه‌هاي خوني نظير كلسترول، تري‌گليسيريد، وي ال دي ال و اچ دي ال و همچنين فعاليت آنزیمهاي كبدي نظير آلكالين فسفاتاز (ALP)، آلانينترانسآميناز (ALT) و آسپارتات ترانس‌آميناز (AST) بررسي شد. برای ارزيابي بافت و جمعيت فلور ميكروبي روده باريك، نمونهبرداري صورت گرفت. نتايج نشان داد مصرف جيرههاي مكمل شده با گوانيدينواستيك اسيد سبب بهبود معنيدار صفات لاشه در بلدرچین‌هاي تحت مطالعه شد (05/0 ≤ p). مكملسازي جيره با گوانيدينو استيك اسيد سبب كاهش معنيدار بر غلظت سرمي فراسنجه‌هاي خوني كلسترول، تري گليسريد و LDL و متعاقبا افزايشHDL شد (05/0 ≤ p). بهبود وضعيت فعاليت آنزیمهاي كبدي تحت تاثير مصرف سطوح گوانيدينواستيكاسيد مشاهده گرديد (05/0 ≤ p). افزايش معنيدار جمعيت ميكروبي مفيد لاكتوباسيلوسها و در مقابل كاهش جمعيت كلوني باكتريهاي بيماريزاي اشرشیاكلي و سالمونلا انتريكا در روده همراه با افزايش سطح ويليها و تراكم تعداد سلول‌هاي گابلت نشاندهنده اثر مكملسازي سطوح مختلف گوانيدينو استيك اسيد بر بهبود وضعيت سلامت روده در بلدرچین‌هاي تحت مطالعه بود (05/0 ≤ p). نتايج اين مطالعه ننشانگر سودمندي مصرف سطوح مختلف گوانيدينو استيكاسيد بر صفات لاشه، فراسنجه‌هاي بيوشيميايي خون، آنزیمهاي كبدي، ريختشناسي و جمعيت ميكروبي روده در بلدرچین‌هاي ژاپني بود.
In order to investigate the effect of different levels of Guanidinoacetic acid supplementation supplemneted diets on carcass traits, some blood bichemical and liver enzyme activiies, morphology, intestinal microbial population in broiler Japanese quails, 288 Japanese quails aged 1-35 days with 4 experimental treatments including the control group and those consuming Guanidinoacetic acid at levels of 0.5 and 1 and 1.5 g/kg of standard feed was used with 6 repetitions and 12 pieces of quail in each repetition. At the end of the period (35 days), after killing two quails from each repetition, the cracar traits, blood bichemicals and the liver enzymes activity indices such as alkaline phosphatase (ALP), alanine transaminase (ALT) and aspartate transaminase (AST) were investigated. Additionally, the morphology and intestinal microbial population were examined. The results showed that the Guanidinoacetic acid supplementation supplemented diets caused a significant increase in the thigh, wing, breast and abdominal fat wieght.the result showd that cholestrol, triglycirdie and LDL serum content has decreased and HDL content has increased significantly (p ≤ 0.05). in current study the significant improvement of the liver enzyemes activity has been assayed (p ≤ 0.05). The significant increase of beneficial microbial flora of Lactobacillus and decrease of the colony population of Escherichia coli and Salmonella enterica in the intestine along with the increase in the surface of the villi and the density of the number of goblet cells indicated the beneficial effects of the Guanidinoacetic acid supplementation used in improving the health status of quails (p ≤ 0.05).In conclusion we could demonestare that the benefits of Guanidinoacetic acid supplementation on carcass traits, some blood biochemical, intestinal morphology and intestinal microbial flora in broiler Japanese quails.
1. Ahmadipour, B., Khajali, F., Sharifi, M.R. 2018. Effect of guanidinoacetic acid supplementation on growth performance and gut morphology in broiler chickens. Poultry Science Journal, 6:19-24.
2. Al-Daraji, H.J., Salih, A.M. 2012. Effect of dietary L-arginine on carcass traits of broilers. Animal Veterinary Science, 2:40-44.
3. AOAC. 2005. Official methods of analysis, Association of official analytical chemists. AOAC Press, Gaithersburg, USA.
4. Darmankohi, H., Tavakoli, P., Mohit, A., Mohammadpour, F. 2017. Effects of dietary supplementation of Guanidino acetic acid and L-arginine on performance, blood metabolites and carcass characteristics of Japanese quails. Animal Production Research, 8.9.2:1-10.
5. Davis, S.L. 2011. Plasma levels of prolactin, growth hormone, and insulin in sheep following the infusion of arginine, leucine and phenylalanine. Endocrinology, 91:549-555.
6. Dilger, R.N., Bryant-Angeloni, K., Payne, R.L., Lemme, A., Parsons, C.M. 2013. Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks. Poultry Science, 92:171-177.
7. Duncan, D.B. 1995. Multiple range test and F-test. Biometrics, 11:1-42.
8. Ebrahimi, M., Zare, A., Shivazad, M., Ansari Pirsaraei, Z., Tebyanian, M., Adib Moradi, M., nourijelyani, K.2015. The Effects of L-arginine Supplement on Growth, Meat Production, and Fat Deposition in Broiler Chickens. Iranian Journal of Animal Science Research, 5(4):281-290.
9. Fathi., M. 2015. Effects of L-Arginine levels supplementation on some blood parameters, thyroid hormones, mortality and performance of broilers with cold-induced ascites. Research Journal of Livestock Science, 107:195-206.
10. Fernandes, J.I.M., Murakami, A.E., Martins, E.N., Sakamoto, M.I., Garcia, E. R.M. 2009. Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science, 88:1399-1406.
11. Fouad, A.M., El-Senousey, H.K., Yang, X.J., Yao, H. 2013. Dietary L-arginine supplementation reduces abdominal fat content by modulating lipid metabolism in broiler chickens. Animal, 7:1239-1245.
12. Halle, I., M. Henning and P. Kohler. 2006. Studies of the effects of creatine on performance of laying hens, on growth and carcass quality of broilers. Landbauforschung Volkenrode, 56:11-18.
13. Khalil, S., Saenbungkhor, N., Kesnava, K., Sivapirunthep, P., Sitthigripong, R., Jumanee, S., Chaosap, C. 2021. Effects of guanidinoacetic acid Supplementation on Productive performance, pectoral myopathies, and meat quality of broiler chickens. Animals, 11(11):3180.
14. Khajali, F., Wideman R.F. 2010. Dietary arginine: Metabolic, environmental, immunological andphysiological interrelationships. World’s Poultry Science Journal, 66: 751-766.
15. Lemme, A., Ringel, J., Rostagno, H.S., Redshaw, M.S. 2007. Supplemental guanidino acetic acid improved feed conversion, weight gain, and breast meat yield in male and female broilers.16th European Symposium on Poultry Nutrition, pp:26-30.
16. Lev, R., Spicer, S.S. 1964. Specific staining of sulphate groups with Alcian blue at low pH. Journal of Histochemical and Cytochemistry, 12:309-310.
17. Michiels, J., Maertens, L., Buyse, J., Lemme, A., Rademacher, M., Dierick, N.A. De Smet, S. 2012. Supplementation of guanidinoacetic acid to broiler diets: Effects on performance, carcass characteristics, meat quality, and energy metabolism. Poultry Science, 91:402-412.
18. Mousavi, S.N., Afsar, A., Lotfollahian, H. 2013. Effects of guanidinoacetic acid supplementation to broiler diets with varying energy contents. Poultry Science, 22:47-54.
19. Nasiroleslami, M., Torki, M., Saki, A.A., Abdolmohammadi A.R. 2018. Effects of dietary guanidinoacetic acid and betaine supplementation on performance, blood biochemical parameters and antioxidant status of broilers subjected to cold stress. Journal of Applide Animal Research, 46:1016-1022
20. NRC., National Research Council. 1994. Nutrient requirements of poultry.9th Rev.Ed., Washington, DC. National Academy Press.
21. Ostojic, S.M. 2015. Advanced physiological roles of guanidinoacetic acid. European Journal of Nutrition, 54:1211-1215.
22. Rahimian, Y., Kheiri, F., Faghani, M. 2024. Evaluation the effect of dietary vitamin E, sesamin and thymoquinone bioactive compounds on immunological response, intestinal traits and MUC-2 gene expression in broiler Japanese quails (Coturnix japonica). Animal Biotechnology, 35(1):1-11.
23. Raei, A., Karimi, A., Sadeghi, A. 2019. Performance, antioxidant status, nutrient retention and serum profile responses of laying Japanese quails to increasing addition levels of dietary guanidinoacetic acid. Italian Journal of Animal Science, 19(1):75-85.
24. Ringel, J., Lemme, A., Araujo, L.F. 2008. The effect of supplemental guanidinoacetic acid in Brazilian type broiler diets at summer conditions. Poultry Science, 87:154.
25. SAS Institute. 2001. SAS.STAT user’s guide for personal computer. Release 6.12 SAS Institute, Inc., Cary, N.C., USA.
26. Tossenberger, J., Rademacher, M., Nemeth, K., Halas, V., Lemme, A. 2016. Digestibility and metabolism of dietary guanidinoacetic acid fed to broilers. Poultry Science, 95:2058-2067.
27. Vali N. 2008. The Japanese quail. A Review. International Journal of Poultry Science, 7:925-931.
28. Wang, L., Shi, B., Shan, A., Zhang, Y. 2012. Effects of guanidinoacetic acid on growth performance, meat quality and antioxidation in growing-finishing pigs. Journal of Animal Veterinary Advances, 11:631-636.
29. Wu, L.Y., Fang, Y.J., Guo, X.Y. 2011. Dietary L-arginine supplementation beneficially regulates body fat deposition of meat type ducks. British Poultry Science, 52: 221-226.
30. Wyss, M., Kaddurah-Daouk, R. 2000. Creatine and creatinine metabolism. Physiological Review, 80:1107-1213.