تاثیر شش هفته تمرین تناوبی شدید و کورکومین بر سطوح گلوکز و بیان ژن BMP-2 در عضله صاف عروقی موش‌های صحرایی نر دیابتی
محورهای موضوعی : فصلنامه زیست شناسی جانوری
1 - گروه فیزیولوژی ورزشی دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 - گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد اهواز، ایران
کلید واژه: تمرین تناوبي, BMP2 , كوركومين, ديابت,
چکیده مقاله :
مطالعه ي حاضر با هدف بررسي تاثير شش هفته تمرين تناوبي و مصرف كوركومين بر بیان ژن BMP2 در عضله صاف عروقی موش‌هاي صحرايي نر نژاد ويستار انجام شد. در يك كارآزمايي تجربي تعداد 30 سر موش صحرايي نر ويستار به طور تصادفي به شش گروه (هر گروه 5 سر موش) كنترل، ديابت، سالم تمرين تناوبي، ديابت تمرين تناوبي، ديابت كوركومين و ديابت تمرين كوركومين تقسيم شدند. ديابت با تزريق درون صفاقي سم استرپتوزوتوسين (50 ميليگرم به ازاي هر كيلوگرم) القاء شد. تمرين تناوبي شامل 12-5 وهله كار شديد (75 تا 100 درصد بيشينه سرعت) 60 ثانيهاي با فواصل استراحت فعال 75 ثانيه اي شش روز در هفته به مدت شش هفته بود. كوركومين با دوز 50 ميليگرم در كيلوگرم از وزن بدن به روش گاواژ به حيوانات خورانده شد. پس از شش هفته حيوانات بي هوش و نمونه‌هاي خوني از قلب آنها جمعآوري شد. دادهها با استفاده از آزمون آماري ANOVA و آزمون تعقيبي بن فروني تحليل شدند. القاء ديابت بیان ژنBMP2 در عضله صاف عروقی موش صحرایی نر را افزایش داد. تمرين تناوبي بیان ژن BMP2 عضله صاف عروقی موش مبتلا به دیابت را کاهش داد. شش هفته مصرف كوركومين بیان ژن BMP2 عضله صاف عروقی موش مبتلا به دیابت را كاهش داد. به طور كلي نتايج مطالعهي حاضر نشان داد كه تمرين تناوبي و كوركومين، اختلالات ديابت ناشی از بیان ژن BMP2 و کلسیفیکاسیون و عوارض آن را بهبود ميدهند.
The present study was conducted with the aim of investigating the effect of six weeks of intermittent exercise and curcumin consumption on BMP2 gene expression in the vascular smooth muscle of male Wistar rats. In an experimental trial, 30 male Wistar rats were randomly divided into six groups (5 rats in each group): control, diabetic, healthy intermittent exercise, intermittent exercise diabetes, curcumin diabetes and curcumin exercise diabetes. Diabetes was induced by intraperitoneal injection of streptozotocin poison (50 mg/kg). Intermittent training consisted of 5-12 bouts of intense work (75 to 100% of maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. Curcumin was fed to animals by gavage at a dose of 50 mg per kilogram of body weight. After six weeks, unconscious animals and blood samples were collected from their hearts. The data were analyzed using ANOVA statistical test and Benferroni post hoc test. Induction of diabetes increased BMP2 gene expression in vascular smooth muscle of male rats. Intermittent training decreased BMP2 gene expression in vascular smooth muscle of diabetic rats. Six weeks of curcumin consumption reduced the expression of BMP2 gene in vascular smooth muscle of diabetic rats. In general, the results of the present study showed that intermittent exercise and curcumin improve diabetes disorders caused by BMP2 gene expression and calcification and its complications.
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