بررسی تغییرات ناشی از استرس اکسیداتیو در نتیجه اعمال استرس اجتماعی در دو بافت مغز و کلیه
محورهای موضوعی : فصلنامه زیست شناسی جانوریایراندخت زینائی 1 , شهربانو عریان 2 , محمدرضا واعظ مهدوی 3 , اکرم عیدی 4 , مهرداد روغنی 5
1 - گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران
3 - گروه فیزیولوژی، دانشکده علوم پزشکی شاهد، تهران، ایران
4 - گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
5 - گروه فیزیولوژی، دانشکده علوم پزشکی شاهد، تهران، ایران
کلید واژه: گلوتاتیون, نیتریک اکساید, استرس اجتماعی, آنتی اکسیدان زردچوبه, محدودیت مشاهده تغییر هم خانه,
چکیده مقاله :
ت تاثیر استرس های اجتماعی بر بروز بیماریهای قلبی – عروقی و بیماریهای روحی روانی روشن است. برای بررسی تاثیر گذاری این استرسها بر القائ استرس اکسیداتیو ایجاد تغییر در سطح اکسیدانی و آنتی اکسیدانی مدل محدودیت تغییر هم خانه مشاهده در حضور دریافت آنتی اکسیدان زردچوبه طراحی شد. 60 سر رت نر نژاد ئویستار تحت شرایط استاندارد دما و نور و آب قرار گرفتند و در گروههای تحت استرس یک سوم غذای معمول هر رت در اختبارش قرار داده شد. استرس تغییر هم خانه و مشاهده هم دردو گروه این رتها القا گردید. دردو گروه غذای مصرفی شان محتوی زرد چوبه بود.به مدت 4 ماه رته تحت شرایپط تعریف شده برای هر گروه یعنی تغییر هم خانه مشاهده زردئچوبه ،محدودیت غذایی مشاهده تغییر هم خانه زردچوبه،کنترل،کنترل زردچوبه نگهداری شدند .پس از اتمام دوره نگهداری رتها با اتر بیهوش شده و بافت کلیه و مغز آنها هموژن شد. هر کدا از فاکتور های مرو نظر یعنی مالون دیآلدئید،گلوتاتیون، نیتریک اکساید،کاتپسین D و لیپوفوشین در بافتها سنجش شد. سطح مالون دی آلدئیدبطور معنی داری هم در مغز و هم در کلیه در گروه تحت استرس افزایش پیداکرد. سطح نیتریک اکساید و گلوتاتیون در مغز افزایش یافت میزان فعالیت کاتپسین D و تجمع لیپوفوشین تغییر ی نداشت. مغز نسبت به تشکیل MDA حساس تر است اما سطح گلوتاتیون و نیتریک اکساید در آنها بالاتر است. تغییرات ناشی از القائ استرسهای اجتماعی در دو بافت مغز و کلیه بصورت یکسان رخ نمی دهد.
The effect of social stress on the occurrence of cardiovascular and mental diseases is obvious. In order to investigate the effect of these stresses on oxidative stress induction, and changes in the oxidant and antioxidant levels, the model for roommate change restriction and observation in the presence of curcuma longa antioxidant was designed A total of 60 Wister rats were kept under standard conditions of temperature, light, and water. In the under-stress groups, each rat was provided with one third of its common food. The roommate change stress and observation were applied to two groups of these rats. Two groups of rates were fed by the food contained curcuma longa. These rats kept under defined conditions for each group for 4 months i.e., roommate change, observation, curcuma longa; food restriction, observation, roommate change, curcuma longa; control; control, curcuma longa. After the rats' maintenance period was completed, they were anesthetized with ether and their kidney and brain tissues were homogenized. Each of the given factors i.e., malon-dialdehyde, glutathione, nitric oxide, cathepsin D and lipofuscin was measured in tissues. The malon-dialdehyde level increased significantly both in brain and kidney in under-stress group. The level of nitric oxide and glutathione increased in brain, but the activity of cathepsin D and lipofuscin accumulation remained unchanged. Also the amount of nitric oxide and glutathione remained the same. The brain is more sensitive to MDA formation, but the amount of glutathione and nitric oxide is higher. Thus social stress induced changes do not occur equally in kidney and brain tissues.
1.Vaez Mahdavi M.R., Mojarab S.H., Tarihi T., Roghani M., Faghihzadeh S., Hashempour-Ezati M., 2010. The effect of food deprivation, social statusand inequality on myocardial cell of aging in male rabbits. Daneshvar Medicine Journal, 17(86): 11-18.
2. Kaplan J.R., Manuck S.B., Lusso F.M., Taub D.M., 1982. Social status, environment and atherosclerosis in cynomolgus Monkey. Arteriosclerosis, 2(5): 359-682.
3. Ahmad R., Tripathi K., Tripathi P., Singh S., Singh R, Siingh, R.K., 2008. Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia.Invivo, International Society for the Study of Comparative Oncology, 22(4): 525-258.
4.Halliwell B., Gutteridge J.M.C., (1999). Free Radicals in Biology and Medicine. 3rd Edition, Oxford University Press, 1-25.
5.Sivonova M., Tatarkova z., Duurackova Z., 2007. Relationship between antioxidant potential and oxidative damage to lipids, proteins and DNA in aged rats, Physiological Research, (6): 757-764.
6.Drigen R., 2000. Metabolism and functions of glutathione in brain. Progress in Neurobiology, 62: 649-671.
7. Kazemi M., Marandi S.M., Movahedian Attar A., Mohammadian H., Sharifi Jebeli HR., 2018. Action of L- Arginin on oxidative - nitrosative stress induced by acute exercise in liverof rats. Medical Journal of Tabriz University oF Medicl Science, 40(2): 65-71.
8.Radha K., Maheshwari A.K., Jaya S., 2006. Multiple biological activities of curcumin. A short review. Life Science, 78(18): 2081-2087.
9.Manikandan P., Sumitra M., Aishvarya S., Manohar B.M., 2004. Curcumin modulates free radical quenching in myocardial ischemia in rats. International Journal of Biochemistry & Cell Biology, 36 (10): 1967-80.
10.Hung M., Lysz T., Ferraro T., Abidi T.T.F., Laskin J.D., conney A.H., 1991. Inhibitory effects of curcumin on in vitro lipoxy genase and cyclooxygenase activities in mouse epidermis. Cancer Reserch, 51(3): 813-819.
11.Natio M., Wu X., Normura H., Kodama M., 2002. The protective effect of tetrahydrocurcumin on oxidative stress in cholesterol – fed rabbits . Journal of Atherosclerosis and Thrombosis, 9(5): 243-250.
12.Sun J., Zhang X., Broderick M., Fein H., 2003. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors, 3(8): 276-284.
13.Encinas J.M., Manganas L., Enrkolopov G., 2005. Nitric oxide and multiple scelerosis. Current Neurology and Neuroscience Reports. 5(3): 232-23.
14.Minarowaska A., Miarowski L., 2007. Regulatory role of cath D in apoptosis. ET Cytobiol, 45(3): 159-163.
15.Zhang L., Sheng R., Qin Z., 2009. The lysosome and neurodegeneration diseases. Acta Biochim Biophys Sin (Shanghai), 41(6):437-45.
16.Benes P., Netvicka V., Fusek M., 2008. Cathepsin D-Mny functions of one aspartic protease. Critical Reviews in Oncology/Hematology, 68(1): 12-28.
17.Kagedal K., Johnsson U., Ollinger K., 2001. The lysosomal proteas cathepsin D mediates induced by oxidative stress. FASEB J, 15(9): 1592-4.
18.Jung T., Hohn A., Grune T., 2010. Methods in molecular biology: Lipofuscin detection and quantificcatin by microscope techniques. Springer Protocol, 173-193.
19.Terman A., Brunk U.T., 1998. Lipofuscin: Mechanisms of formation and increase with age. Acta pathologica, microbiologica, immunologica Scandinavica (APMIS), 106(2):265-76.
20.Almorth B., Struve J., Berglund A., 2005. Oxidative damage in fish used as biomarker in field and laboratory studies. Aquatic Toxicology, 73(2):171-80.
21.Ayoubi A., Valizadeh R., Omidi A., Abolfazli M., 2014. Evaluation of Turmeric (Curcuma longa) effects in preventing consequences of lead acetate in male rats. Journal of Birjand University of Medical Sciences, 21(1): 68-76. (In Persian).
22.Levay EA., Govic A., Penman J., Paolini AG., Kent S., 2007. Effect of adult – onset caloric restriction on anxiety – like behavior in rats. Phsiology and Behavior, 92(5): 889-96.
23.Owen J.B, Buttertfield D.A., 2010. Meaesurment of oxidized/reduced glutation ratio. Methods in Molecular Biology, 648: 269-77.
24.Brunk UT., Eur J., 2002. Biochem. The mitochondrial lysysom axis theory of aging accumulation of damage mitochondria as a result of imperfect autophagosytosis, 269(8): 1996-2002.
25.Zeraatpishe A., Oryan SH., Bagheri MH., Pilevarian AA., Malekirad AA., Baeeri M., Abdollahi M., (2011). Theeffect of Melissa officinalisl. (Lemonbalm) infusion on enzymatic antioxidants activity in radiology staff . Toxicology and industrialhealth, 27(3): 205-212.
26.Malekirad A.A., Oryan S., Fani A., Babapour V., 2010. Study on clinical and biochemical toxicity biomarkers in zinc – lead mine workers. Toxiocology Industrial Health, 26(6): 331-337.
27.Oparinde DP., Salawu AA, Atiba AS, Duduyemi BM., 2013.Significance of body weight measurement along with malondialdehyde :Review of published research articles. Key Research Journal of Biotechnology, 1(1):1-3.
28.Kasperska Z.A., Brzoza Z., Rogala B., Polaniak R., Brikner E., 2006. Antioxidant enzyme activity and malondialdehyde concentration in the plasma and erythrocytes of patient with urticarial induced by nonsteroidal anti-inflammatory drugs. Journal of Investigational Allergology and Clinical Immunology, 18(5): 327-375.
29.Bian K., Dourosout M.F., Murad F., 2008. Vascular system: Role of nitric oxide in cardiovascular disease. Journal of clinical hypertension (Greenwich), 10(4): 304- 310.
30.Girouard H., Wang G., Gallo E.F., 2009. NMDA receptor activation increase free radicalsproduction through nitric oxide and NOX2. The journal of neuroscience. The Official Journal of The Society for Neuroscience, 29(8): 2545-52.
31.Calabrese V., Mancuso C., Calvai M., Rizzarelli E., Butterfield A.D., Stella A.M., 2007. Nitric oxide in central Nervous syetem. Nature reviews, Neuroscience, 8(10): 766-75.
32.Rodrigo J., Femandez A.D., Alosso D., Serrano J., Femandez–vizarra P., Martinez – Murrillo K., 2004. Nitric oxide in the rat cerebellum after hypoxia / ischemia. Cerebellum (London, England), 3(4): 194-203.
33.Nath N., Morinanga O., Singh I., 2010. S-nitrosoglutathione a physiologic nitric oxide carrier attenuates experimental autoimmune encephalomyelitis. Journal of Neuroimmune Pharmacology, 5(2): 240-251.
34.Nishikawa M., Sato E.F., Kuroki T., Utsumi K., 1998. Inue apoptosis of rat hepatoma ceu invivo. Hepatology, 28(6): 1474-1480.
35.Kim Y.M., Chang H.T., kim S.S., Han J.A., Yoo Y.M., Kim K.M., 1999. Nitric oxide protects PCL2 cells from serum deprivation – induced apptosis by cGMP – dependent inhibition of caspase signaling.The journal of neuroscience. The Official Journal of the Society for Neuroscience, 19(16): 6740-6747.
36. Rezaei S., Vaez Mahdavi M.H., Noorbala A.A., Roghani M., Faghih Zadeh S., Effect of food restriction, food deprivation and food inequality on anxiety-like behavior in rats. Daneshvar Medicine Journal, 21(112): 35-45. (In Persian).
37.Lu S.C., 2009. Regulation syntethesis. Moleular Aspect of Medicine, 30(1-2): 42-59.
38.Valipour1 A., Valipour M., A review of Curcumin and its role in disease prevention and treatment Clinical Excellence, 6(1): 35-54. (In Persian).
39.Mahdidoost S., Vaeez Mahdavi M.R., Kabvdanian Ardestani S., Sedaghat R., Jalilvand M., Khalili M., 2012. The effect of stress due to food deprivation social inequality and instability and quantityive lipofuscin in brain. Iranian Society of Physiology and Pharmacology, 16(4): 350-359.
40.Mojarab S.H., 2010.Effect of food inequality and unstable socialstatus on myocardial cell of male rabbits. Daneshvar Medicine, 17(86): 1-10. (In Persian).
41.Heydari F., Vaeez Mahdavi M.R., Minaei B., Roghani M, Fallah N., Heydary R., Gharebaghi K., 2008. Food inequality negatively impacts on cardiac health in rabbits. Plos one, 3(11): 370-375.
42.Vaez Mahdavi M.R., Roghani M., Khalili M., Dalir R., 2009. The effect of food restriction on learning and memory of male wister rat. Behavorial analysis Basic and Clinical Nature Scienc,1(2): 20-23.
_||_