اثرات اسید سالیسیلیک روی شاخص‌های رشدی و برخی از نشانگرهای بیوشیمیایی گیاهچه‌های گندم L.) Triticum aestivum) تحت تنش شوری
محورهای موضوعی : بوم شناسی گیاهان زراعیالهام قافیه سنج 1 , کمال الدین دیلمقانی 2 , حسن حکمت شعار 3
1 - 1- کارشناس ارشد علوم گیاهی، دانشگاه آزاد اسلامی واحد مرند، مرند، ایران.
2 - استادیار گروه علوم گیاهی، دانشگاه آزاد اسلامی واحد مرند، مرند، ایران.
3 - دانشیار گروه علوم گیاهی، دانشگاه آزاد اسلامی واحد مرند، مرند، ایران.
کلید واژه: پروتئین, پرولین, شوری, اسید سالیسیلیک, شاخص&lrm, های رشد, مالون دی آلدهید,
چکیده مقاله :
به منظور بررسی اثر اسید سالیسیلیک بر پارامترهای رشد (وزن تر اندام هوایی و ریشه)، پرولین، پروتئین محلول و مالون دی آلدهید در دانهرستهای گندم رقم زرین تحت تنش شوری کلرید سدیم، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار و برای هر ترکیب تیماری در 27 واحد آزمایشی انجام گرفت. فاکتور اول شوری در سه سطح (0، 75، 150 میلی‌مولار) و فاکتور دوم اسید سالیسیلیک در سه غلظت 0، 200، 400 میلیگرم بر لیتر بودند. هشت روز پس از کشت در مرحله دو برگی کامل، گیاهان تیمار NaCl 0، 75 و 150 میلی‌مولار کلرید سدیم را دریافت کردند. شوری 75 میلی‌مولار طی دو نوبت با غلظت‌های 25 و 50 میلی‌مولار و شوری 150 میلی‌مولار طی چهار نوبت با غلظت‌های 25، 50، 25 و 50 میلی‌مولار به فاصله یک هفته از یکدیگر اعمال شد. هفت روز پس از اعمال تیمار شوری، گیاهان تیمار اسید سالیسیلیک 0، 200 و 400 میلی گرم برلیتر را در سه تکرار، طی دو نوبت و به فاصله شش روز از یکدیگر به صورت افشانه برگی دریافت کردند. نتایج نشان داد که با افزایش شوری، مقادیر پروتئین، وزن تر اندام هوایی و ریشه کاهش، ولی مقادیر پرولین و مالون دی آلدهید افزایش یافت. کاربرد اسید سالیسیلیک برون‌زا مقادیر پروتئین، وزنتر اندام هوایی و ریشه را افزایش داد، ولی مقدار پرولین و مالون دی‌‌آلدهید در حضور شوری کاهش یافت. کاربرد اسید سالیسیلیک برون‌زا در نبود شوری بر میزان پرولین و مالون دی آلدهید تاثیری نداشت.
In order to investigate the effect of salicylic acid on growth parameters (shoot and root fresh weight) and some biochemical markers including proline, soluble protein and malondialdehyde levels in wheat seedlings under salt stress, a factorial experiment based on a completely randomized design was conducted with three replications. Factors were salinity at three levels (0, 75 and 150 mM. NaCl ) and salicylic acid at three levels (0, 200 and 400 mg.l-1). Eight days after planting and in complete bifoliolate stage, salt stress treatments were applied. Concentration of 75 mM NaCl was applied by concentrations of 25 and 50mM during two times with seven days interval. NaCl 150 mM was also applied by concentrations of 25, 50, 25 and 50 mM during four times with seven days interval. Seven days after exerting the salt stress, leaves were sprayed by concentrations of 0, 200 and 400 mg.l-1 salicylic acid, with three replications, during two times with six days interval. Based on the results, the amount of protein, shoot and root fresh weight reduced with salinity increasing, but proline and malondialdehyde amounts increased. Exogenous salicylic acid application increased protein, shoot and root fresh weight contents, but reduced proline and malondialdehyde in presence of salinity. However, exogenous salicylic acid application in normal condition stress have not any effect on proline and malondialdehyde contents.
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