اثر هشت هفته تمرین مقاومتی پس از پیوند سلول های بنیادی مشتق از بافت چربی بر برخی فاکتورهای آپوپتوزی و ضد آپوپتوزی بافت قلب در موش های مدل کبد چرب غیرالکلی
محورهای موضوعی : اثر فعالیت بدنی بر بیماری ها
عرفان غلامی
1
(دانشجوی دکتری فیزیولوژی ورزشی،گروه فیزیولوژی ورزش، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران)
سید عبداله هاشم ورزی
2
(استادیار گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران)
سیده یاسمن اسدی
3
(استادیار گروه تربیت بدنی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران)
کلید واژه: تمرین مقاومتی, سلول بنیادی, آپوپتوز, کبد چرب,
چکیده مقاله :
زمینه و هدف: بیماری کبد چرب غیرالکلی یکی از مهمترین اختلالات مزمن کبدی است که احتمالا با مشکلات قلبی نیز مرتبط است. پژوهش حاضر، با هدف بررسی اثر هشت هفته تمرین مقاومتی پس از پیوند سلول بنیادی بر فاکتورهای آپوپتوزی و ضد آپوپتوزی بافت قلب در موش‌های مدل کبد چرب انجام شد. مواد و روش‌ها: 36 سر موش، به دو گروه کنترل (6 سر) و بیمار کبد چرب (30 سر) تقسیم شدند. گروه کنترل 6 هفته از غذای استاندارد و گروه‌های بیمار، از غذای پرچرب جهت القای بیماری کبد چرب استفاده کردند. در پایان هفته ششم، جهت تایید ایجاد بیماری کبد چرب، به طور تصادفی از دم 3 سر موش خونگیری و آنزیم های کبدی اندازه گیری شد. سپس حیوانات گروه بیمار به طور تصادفی به 5 گروه سالین، کبد چرب، تمرین، سلول و تمرین + سلول تقسیم شدند. به گروه‌های دریافت کننده سلول، حدود 106×5/1 عدد سلول بنیادی از طریق سیاهرگ دمی تزریق گردید. برنامه تمرینی شامل 8 هفته صعود از نردبان بود. نتایج: مقادیر Bax و Bcl-2 بافت قلب در گروه‌های درمان نسبت به گروه بیمار به ترتیب کاهش و افزایش یافت اما این تغییرات تنها در گروه تمرین و تمرین + سلول نسبت به گروه بیمار معنی‌دار بود. نتیجه‌گیری: بیماری کبد چرب غیرالکلی به طور چشمگیری با آپوپتوز سلول‌های قلبی همراه است. تمرین مقاومتی پس از پیوند سلول بنیادی سبب کاهش مقادیر Bax و افزایش مقادیر Bcl-2 بافت قلب گردید. بنابراین به نظر می رسد این روش می‌تواند به عنوان یک راهکار درمانی مورد توجه قرار گیرد.
Introduction: Nonalcoholic fatty liver disease is one of the most common chronic hepatic diseases which may be associated with cardiovascular disease. The study was conducted with the aim of investigating the effect of eight weeks of resistance training after stem cell transplantation in fatty liver model rats. Methods: 36 rats were divided into two groups: control (6 rats) and patients with fatty liver (30 rats). The control group used standard food for 6 weeks, while the animals in the diseased groups used high-fat food to induce fatty liver disease. At the end of the sixth week, to confirm the development of fatty liver disease, blood was randomly drawn from the tails of 3 rats. Then the animals of the patient group were randomly divided into 5 groups Saline, Fatty liver, Exercise, Cell, Exercise + Cell. About 1.5 x 106 stem cells for each mouse were injected into the cell receiving groups through the tail vein. The training program consisted of 8 weeks of climbing the ladder. Results: The amounts of Bax and Bcl-2 of the heart tissue decreased and increased respectively in the treated groups compared to the patient group, but these changes were significant only in the exercise group and exercise + cells compared to the patient group. Conclusion: Non-alcoholic fatty liver disease is significantly associated with cardiac cell apoptosis. Resistance training after stem cell transplantation decreased the Bax and increased the Bcl-2 in heart tissue. Therefore, it seems that this method can be considered as a therapeutic solution.
1. Chan WK, Dan YY, Wong VW; GO ASIA Initiative. Editorial: NAFLD in Asia-clinical associations with advanced disease become clearer. Authors' reply. Aliment Pharmacol Ther. 2018: 47(7): 1037-38. doi: 10.1111/apt.14572.
2. Bălănescu A, Bălănescu P, Comănici V, Stan I, Acs B, Prisăcariu L, et al. Lipid profile pattern in pediatric overweight population with or without NAFLD in relation to IDF criteria for metabolic syndrome: a preliminary study. Rom J Intern Med. 2018 Mar; 56(1): 47-54. doi: 10.1515/rjim-2017-0040.
3. Hossein Mahdian, Parvin Farzanegi, Amin Farzaneh Hesari. [Effect of resveratrol supplementation and interval exercise on heart cells apoptosis in male rats with non-alcoholic fatty liver]. J Gorgan Univ Med Sci. 2020 Winter; 21(4): 39-46. [Article in Persian].
4. Norouzpour M, Marandi M, Ghanbarzadeh M, Zare Mayavan A. The effect of com¬bined training on serum concentrations of inflammatory cytokines and factors associated with metabolic syndrome in elderly women with fatty liver. Journal of Sport and Exercise Physiology 2022;15(2):64-75 (Persian).
5. Kate Hallsworth, Christian Thoma, Sarah Moore, Thomas Ploetz, Quentin M Anstee, Roy Taylor, Christopher P Day, Michael I Trenell. Non-alcoholic fatty liver disease is associated with higher levels of objectively measured sedentary behaviour and lower levels of physical activity than matched healthy controls. Frontline Gastroenterology 2015; 6:44–51.
6. Shira Zelber-Sagi, Justyna Godos and Federico Salomone. Lifestyle changes for the treatment of nonalcoholic fatty liver disease: a review of observational studies and intervention trials. Therapeutic Advances in Gastroenterolog 2016; 9(3) 392–407.
7. Pollack M, Phaneuf S, Dirks A, Leeuwenburgh C. The role of apoptosis in the normal aging brain, skeletal muscle, and heart. Ann N Y Acad Sci. 2002 Apr; 959: 93-107. doi: 10.1111/j.1749-6632.2002.tb02086.x
8. Loomba R, Friedman SL, Shulman GI. Mechanisms and disease consequences of nonalcoholic fatty liver disease. Cell 2021;184(10):2537–64.
9. Chalasani N, Younossi Z, Lavine E J, Diehel M A, Brunt M E, Cusi K, et al. The Diagnosis and Management of Non-alcoholic Fatty Liver Disease: Practice Guideline by the American Gastroenterological Association, American Association for the Study of Liver Diseases, and American College of Gastroenterology 2012 by the AGA Institute. Am J Gastroenterol 2012. (142):1592–609.
10. Hajighasem A, Farzanegi P, Mazaheri Z. Effects of combined therapy with resveratrol, continuous and interval exercises on apoptosis, oxidative stress, and inflammatory biomarkers in the liver of old rats with non-alcoholic fatty liver disease. Arch Physiol Biochem. 2019 May; 125(2): 142-49. doi: 10.1080/13813455.2018.1441872.
11. Youkun Bi, Xuejun Guo, Mengqi Zhang, Keqi Zhu, Chentao Sh, Baoqi Fan, Yanyun Wu, Zhiguang Yang and Guangju Ji. Bone marrow derived‑mesenchymal stem cell improves diabetes‑associated fatty liver via mitochondria transformation in mice. Stem Cell Research & Therapy (2021): 12:602.
12. Pourheydar B , Shahi M, Farjah GH, Javanmard M, Karimipour M, Atabaki F. Evaluation of apoptosis in hippocampal cells of rat following intravenous injection of bone marrow stromal cells in ischemia-reperfusion model. Studies in Medical Sciences 2014, 25(7): 586-597.
13. Efati M, Khorrami M, Zarei Mahmmudabadi A, Raouf Sarshoori J. Induction of an animal model of non-alcoholic fatty liver disease using a formulated high-fat diet. J Babol Univ Med Sci 2017;18(11):57-62 (Persian).
14. Hajighasem A, Farzanegi P, Mazaheri Z, Naghizadeh M, Salehi Gh. Effects of resveratrol, exercises and their combination on Farnesoid X receptor, Liver X receptor and Sirtuin 1 gene expression and apoptosis in the liver of elderly rats with nonalcoholic fatty liver. Peer J 2018; 6:5522: 1-15.
15. Hamidi Perchikolaei S.O, Hashemvarzi S.A, Pourghasem M. The Effect of 6 Weeks Progressive Aerobic Training with Injection of Adipose Tissue-Derived Stem Cells on Nerve Growth Factor and Vascular Endothelial Growth Factor Levels in Streptozotocin-Induced Diabetic Rat’s Hippocampus. Sport Physiology. Fall 2019; 11(43):123-40. (In Persian). DOI: 10.22089/spj.2019.7401.1907.
16. Taha MF, Hedayati V. Isolation, identification and multipotential differentiation of mouse adipose tissue derived stem cells. Tissue Cell. 2010;42(4):211-16.
17. Moradi A, Mohammadi S, Hamidi Alamdari D. Effect of adipose tissue-derived stem cells on the control of the blood glucose level in diabetic rats. J Shahid Sadoughi Univ Med Sci. 2015;23(8):717-26. (In Persian).
18. Sadeghi M, Abbassi Daloii A, Ziaolhagh S. Effect of 6 Weeks of Resistance Training and oldenone Supplementation on 5-alpha Reductase and Aromatase Gene Expression in Testes Tissue of Male Wistar Rats. Horizon of Medical Sciences. 2017;23(3):193-199.
19. Shahriari Felordi M, Torabi Sh, Shokoohian B, Farzaneh Z, Mohamadnejad M, Malekzadeh R, Baharvand H, Vosough M. Novel Cell-Based Therapies in Hepatic Disorders. J Mazandaran Univ Med Sci 2020; 30(185): 184-208 (Persian).
20. Shamsoddini A, Sobhani V, Ghamar Chehreh ME, Alavian SM, Zaree A. Effect of Aerobic and Resistance Exercise Training on Liver Enzymes and Hepatic Fat in Iranian Men With Nonalcoholic Fatty Liver Disease. Hepat Mon 2015; 15(10):e31434.
21. Nabizadeh Haghighi A, Shabani R. Comparing effects of medication therapy and exercise training with diet on liver enzymes levels and liver sonography in patients with non-alcoholic fatty liver disease (NAFLD). Journal of University of Medical Sciences 2016; 5(4):488-500.
22. Capitelli CS, Lopes CS, Alves AC, Barbiero J, Oliveira LF, Silva V, Vital M. Opposite Effects of Bone Marrow-Derived Cells Transplantation in MPTP-rat Model of Parkinson’s Disease: A Comparison Study of Mononuclear and Mesenchymal Stem Cells. Int J Med Sci 2014; 11(10): 1049-106.
23. Kang R, Zhou Y, Tan S, Zhou G, Aagaard L, Xie L, Bünger C, Bolund L, Luo Y. Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity. Stem Cell Research & Therapy (2015) 6:144. 1-14.
24. Polgar S, Karimi L, Buultjens M, Morris M, Busse M. Assessing the Efficacy of Cell Transplantation for Parkinson’s Disease: A Patient-Centered Approach. Journal of Parkinson's Disease, 2018; 8(3): 375-383.
25. Wahl P, Brixius K, Bloch W. Exercise-induced stem cell activation and its implication for cardiovascular and skeletal muscle regeneration. Minimally Invasive Therapy & Allied Technologies 2008; 17(2): 91-99.
26. Zhong Z, Chen A, Fa Z, Ding Z, Xiao L, Wu G, Wang Q, Zhang R. Bone marrow mesenchymal stem cells upregulate PI3K/AKT pathway and down-regulate NF-κB pathway by secreting glial\ cell-derived neurotrophic factors to regulate microglial polarization and alleviate deafferentation pain in rat. Neurobiology of Disease 2020; (143); 1-17.
27. Hashemvarzi SA, Heidarianpour A, Fallah Mohammadi Z, Pourghasem M. Synergistic Effects of Aerobic Exercise after Bone Marrow Stem Cell Transplantation on Recovery of Dopaminergic Neurons and Angiogenesis Markers of Parkinsonian Rats. International Journal of Applied Exercise Physiology, 2016, 1 (5):69-80.
