A soft tissue fabricated using a freeze-drying technique with carboxymethyl chitosan and nanoparticles for promoting effects on wound healing
محورهای موضوعی : Journal of Nanoanalysis
Atiyeh Raisi
1
,
Azadeh Asefnejad
2
,
Maryam Shahali
3
,
Zahra Doozandeh
4
,
Bahareh Kamyab Moghadas
5
,
Saeed Saber-Samandari
6
,
Amirsalar Khandan
7
1 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran,
Iran
2 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran,
Iran
3 - Department of Quality Control, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran
4 - Master of Nursing Science, Islamic Azad University, Isfahan (Khorasgan) Branch, Esfahan, Iran
5 - Department of Chemical Engineering, Shiraz Branch, Islamic Azad University, Shiraz, Iran
6 - New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
7 - New Technology Research Center, Amirkabir University of Technology, Tehran, Iran
کلید واژه: Tissue Engineering, soft tissue, freeze drying, Wound dress, Carboxymethyl Chitosan,
چکیده مقاله :
Objective(s): Many people suffer from skin injuries due to various problems such asburns and accidents. Therefore, it is essential to shorten treatment time and providingstrategies that can control the progression of the wound that would be effective inwound healing process and also reduce its economic costs.Methods: The present study aimed to prepare a nanocomposite dressing (NCD)composed of carboxymethyl chitosan (CMC), and Fe2O3 nanoparticles by a methodcalled freeze-drying (FD) technique. The effect of different weight percentages ofFe2O3 (0, 2.5, 5, and 7.5 wt%) reinforcement on mechanical and biological propertiessuch as tensile strength, biodegradability, and cell behavior was evaluated. Also, theX-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis were used tocharacterize the soft porous membrane. The biological response in the physiologicalsaline was performed to determine the rate of degradation of NCD in phosphatebuffer saline (PBS) for a specific time.Results: The obtained results demonstrated that the wound dress was porousarchitecture with micron-size interconnections. In fact, according to the results, asthe magnetite nanoparticles amount increases, the porosity increases too. On theother hand, the tensile strength was 0.32 and 0.85 MPa for the pure sample and thesample containing the highest percentage of magnetic nanoparticles, respectively.Besides, the cytotoxicity of this nanocomposite was determined by MTT assays for 7days and showed no cytotoxicity toward the growth of fibroblasts cells and had properin vitro biocompatibility. The obtained results revealed that NCD had remarkablebiodegradability, biocompatibility, and mechanical properties. Therefore, NCDcomposed of CMC and Fe2O3 nanoparticles was introduced as a promising candidatefor wound healing applications.Conclusions: According to the obtained results, the optimum NCD specimen with 5wt% Fe2O3 has the best mechanical and biological properties.
