Synthesis and evaluation of antibacterial properties of green copper oxide nanoparticles from Hypericum perforatum plant extract and Marrubium Vulgare
محورهای موضوعی : Journal of NanoanalysisAshkan Farazin 1 , Shirin Kavezadeh 2
1 - Department of Solid Mechanics, Mechanical Engineering University of Kashan, Kashan, Iran
2 - Department of Electrical Engineering, University of Isfahan, Iran, P.O. Box 81746-73441
کلید واژه: Hypericum Perforatum, Antibacterial Properties, Scanning electron microscope, Marrubium Vulgare, Industrial antibiotics,
چکیده مقاله :
In the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal ions to nanoparticles as reducing agents, these two plants were tested for their antioxidant properties by the free radical scavenging method, and the IC50 quantity was measured. Hypericum perforatum plant with IC50 equivalent to 0.413 had more antioxidant content than Marrubium Vulgare plant with IC50 equivalent to 1.562, so it was superior in the process of green synthesis. The properties of the synthesized nanoparticles were analyzed using X-ray diffraction (XRD), Scanning electron microscope (SEM), and Ultraviolet-visible (UV-Visible). The presence of a metal-oxygen bond was confirmed by Fourier-transform infrared spectroscopy (FTIR). X-ray energy diffraction spectra showed the purity of the synthesized nanoparticles. The synthesized nanoparticles were observed with spherical morphology and size distribution of 30 to 40 nm and with uniform size distribution. The results of the XRD spectrum showed that pH adjustment did not affect the synthesis of copper oxide nanoparticles. The nanoparticles synthesized against the two bacteria used in this present study did not show significant antibacterial properties compared to industrial antibiotics.
In the present study, copper oxide nanoparticles were synthesized using Hypericum perforatum plant extract from the Malpican family and Marrubium Vulgare plant from the mint family. Since it is believed that the antioxidants in the plant reduce the reduction of metal ions to nanoparticles as reducing agents, these two plants were tested for their antioxidant properties by the free radical scavenging method, and the IC50 quantity was measured. Hypericum perforatum plant with IC50 equivalent to 0.413 had more antioxidant content than Marrubium Vulgare plant with IC50 equivalent to 1.562, so it was superior in the process of green synthesis. The properties of the synthesized nanoparticles were analyzed using X-ray diffraction (XRD), Scanning electron microscope (SEM), and Ultraviolet-visible (UV-Visible). The presence of a metal-oxygen bond was confirmed by Fourier-transform infrared spectroscopy (FTIR). X-ray energy diffraction spectra showed the purity of the synthesized nanoparticles. The synthesized nanoparticles were observed with spherical morphology and size distribution of 30 to 40 nm and with uniform size distribution. The results of the XRD spectrum showed that pH adjustment did not affect the synthesis of copper oxide nanoparticles. The nanoparticles synthesized against the two bacteria used in this present study did not show significant antibacterial properties compared to industrial antibiotics.
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