بررسی و مقایسه اثر روش‌های استخراج با تقطیر و سیال فوق بحرانی بر عملکرد، فعالیت آنتی‌اکسیدانی و ضدقارچی اسانس گیاه دارویی Artemisia persica Boiss.

محورهای موضوعی : گیاهان دارویی

پروین رامک ¹ (مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان لرستان)
وحید کریمیان ² (باشگاه پژوهشگران جوان و نخبگان، واحد یاسوج، دانشگاه آزاد اسلامی)
اصغر سپهوند ³ (مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان)

تاریخ دریافت : 1398/02/18 تاریخ پذیرش : 1398/06/02 تاریخ انتشار : 1398/06/01

کلید واژه: ضد قارچی, Artemisia persica, سیال فوق بحرانی, : آنتی‌اکسیدان,

چکیده مقاله :

دراین مطالعه سرشاخه‌های گلدار درمنه ایرانی (Artemisia persica Boiss.) در فصل تابستان سال 1396، از مزرعه تحقیقاتی نورآباد لرستان برداشت و اسانس گیاهان به روش‌های تقطیر با آب و سیال فوق بحرانی استخراج شد. مهمترین مواد موثره اسانس‌ها با استفاده از دستگاه GC/MS آنالیز گردید و عملکرد آنتی اکسیدانی و ضد قارچی آنها مورد ارزیابی قرار گرفت. با استفاده از روش براث میکرو دایلوشن اثر اسانس‌ها بر روی سویه‌های Microsporum canis ، Trichophyton rubrum، Epidermophyton floccosum و Trichophyton mentagrophytes ، سنجش شد. همچنین فعالیت آنتی‌اکسیدانی اسانس‌ها با دو آزمون مهار رادیکال های آزاد 2 و 2 - دی فنیل- 1- پیکریل هیدرازیل (DPPH) و 2 و 2- آزینو بیس 3 -اتیل بنزوتیازولین -6- سولفونیک اسید (ABTS) ارزیابی گردید. بازده اسانس در روش تقطیر با آب؛ 7/1درصد و در روش سیال فوق بحرانی بین 7/1 تا 7/5 درصد متتغییر بود. عمده‌ترین ترکیبات شناسایی شده در روش سیال فوق بحرانی عبارت بودند از؛ داوانون (29 درصد)، لاسینیاتافوران H (1/18 درصد)، آرتدوگلاسیا اکسید C (5/8 درصد) و نوروداوانه (6/7 درصد) و در روش تقطیر با آب؛ داوانون (22 درصد)، لاسینیاتافوران G (1/7 درصد) و آرتدوگلاسیا اکسید D (6 درصد)، ترکیبات غالب بودند. اسانس استخراج شده به روش تقطیر با آب دارای بیشترین اثر ضد قارچی بر سویه‌های اپیدرموفایتون فلوکوزوم و تریکوفایتون منتاگروفیتز با MFC و MIC معادل μg/mL 32، بود. همچنین اسانس استخراج شده با آب، در آزمون‌های DPPH و ABTS به ترتیب با IC50 ؛ 32/20 و 23/14 میکروگرم بر میلی‌لیتر، بیشترین قدرت مهار رادیکال های آزاد را داشت.

چکیده انگلیسی:

In this study, aerial parts of Artemisia persica in blooming satage were collected from Noor Abad Research Farm (Lorestan province, Iran) in the summer of 2017. Essential oils of plants were extracted by water distillation and supercritical fluid extraction (SFE) methods and were analyzed by using GC/MS device. Antifungal properties of essential oils were evaluated on Microsporum canis, Trichophyton rubrum, Epidermophyton floccosum, and Trichophyton mentagrophytes strains by broth microdilution method. Antioxidant activity of the essential oils was also evaluated by two free radical scavenging tests, viz. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Essential oil yields were 1.7% and 1.7-5.7% in water distillation and SFE methods, respectively. Major compounds identified in the SFE method were Davanone (29%), lacintafuran H (18.1%), C-artedoglacia oxide (8.5%), and neurodevnet (7.6%). Davanone (22%), lacintaphoran G (7.1%), and D-artedoglacia oxide (6%) were the predominant compounds detected in water distillation method. The essential oil extracted by water distillation had the highest antifungal effects on E. floccosum and T. montagrophytes strains with MFC and MIC of 32 µg/mL. Water extracted essential oil presented the highest free radical scavenging capacity in DPPH and ABTS tests with IC50 values of 20.32 and 14.23 μg/ml, respectively.

منابع و مأخذ:

 

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