• صفحه اصلی
  • بررسی فیتوشیمیایی، آنتی‌اکسیدانی و ضدالتهابی استرول‌ها و اسیدهای چرب دو گونه دارویی( Eryngium caucasicum Trautv) و (caucalis platycarpos L) در استان گیلان

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آدرس مقاله


کد مقاله : EJMP-1711-1295 (R6) بازدید : 151 صفحه: 40 - 54

20.1001.1.23223235.1398.7.1.4.7

نوع مقاله: پژوهشی

بررسی فیتوشیمیایی، آنتی‌اکسیدانی و ضدالتهابی استرول‌ها و اسیدهای چرب دو گونه دارویی( Eryngium caucasicum Trautv) و (caucalis platycarpos L) در استان گیلان

محورهای موضوعی : گیاهان دارویی

سحر محمدی پور 1 , عبدالله حاتم زاده 2 , داوود بخشی 3 , اردلان پاسداران 4

1 - گروه باغبانی شاخه گیاهان دارویی، دانشگاه پردیس گیلان، رشت، ایران
2 - استاد، گروه علوم باغبانی، دانشگاه گیلان، رشت، ایران
3 - استاد، گروه علوم باغبانی، دانشگاه گیلان، رشت، ایران
4 - فارماکوگنوزی/ دانشکده داروسازی/ دانشگاه علوم پزشکی شیراز

تاریخ دریافت : 1396/08/16 تاریخ پذیرش : 1397/11/14 تاریخ انتشار : 1398/03/01

کلید واژه: اسیدهای چرب, آنتی‌اکسیدان, ضدالتهابی, زولنگ (E.caucasicum), ساقه سبز(C.platycarpos),

چکیده مقاله :

التهاب واختلالات وابسته به فرآیند اکسیداسیون زمینه ساز بسیاری از بیماری‌های تهدید کننده سلامت عمومی را میباشند. در این تحقیق به بررسی و شناسایی محتوای استرولی و اسیدهای چرب موجود در دو گونه گیاه E.caucasicum و C.platycarpos پرداخته شده است. دو گونه گیاهی یاد شده متعلق به تیره چتریان(Apiaceae) بوده که نقش قابل توجهی در فرهنگ تغذیه‌ ای منطقه خزری دارامیباشند. علاوه بر بررسی فیتوشیمیایی این دو گونه‚ پتانسیل‌های ضد التهابی و آنتی‌اکسیدانی آنها نیز به منظور آماده سازی مکمل‌های غذایی مورد بررسی قرار گرفت. ارزیابی اسیدهای چرب و فیتواسترول‌های این گیاهان مذکوربا روش کروما توگرافی گازی- طیف سنجی جرمی chromatography-Mass spectroscopy مورد ارزیابی قرار گرفت و برای شناسایی ساختار ترکیبات شناسایی شده از مقایسه کروماتوگرام‌های حاصل با اجزای استاندارد، استفاده شد. برای بررسی اثرات آنتی‌اکسیدانی از متدهای زیر بهره گرفته شد: 1)) 1, 1-diphenyl-1-picrylhydrazyl (DPPH) 2) phosphomolybdenum, 2 2’-azino-bis (3-ethylbenzothiazoline)-6-sulphonic acid radical cation (ABTS) )3) cupric ion reducing activity (CUPRAC) )4) Ferric reducing antioxidant power (FRAP) و )5) metal chelating activity on ferrous ions. همچنین برای ارزیابی فعالیت ضد التهابی اسیدهای چرب و فیتواسترول‌های موجود در فراکسیون‌های دو گونه مذکور از روش human red blood cell (HRBC) membrane stabilization method استفاده شد. مقدار کلی روغن این گیاهان به ترتیب 18.21% و 7.39 % بود که درصد روغن موجود در عصاره گیاه E.caucasicum به طور قابل توجهی از C.platycarpos بیشترمیباشد.

چکیده انگلیسی:

Inflammation and oxidative related disorders consist the most important public health threating diseases. This study investigates the fatty acid and sterol contents of E. caucasicum and C. platycarpos, which are part of the nutrition culture of the Caspian region. Their antioxidant and anti-inflammatory potentials are also tested with regard to preparing supplemental foods. The fatty acid and sterol contents are quantified by gas chromatography–mass spectrometry (GC MS), and are identified by comparison to standard components. All fractions were tested for free radical scavenging properties with respect to 1, 1-diphenyl-1-picrylhydrazyl (DPPH), phosphomolybdenum, 2, 2’-azino-bis (3-ethylbenzothiazoline)-6-sulphonic acid radical cation(ABTS), cupric ion reducing activity (CUPRAC), Ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions methods. Furthermore, the anti-inflammatory activity of the fatty acid and sterol fractions of E. caucasicum and C. platycarpos were assayed by the human red blood cell (HRBC) membrane stabilization method. The total oil content of these plants was 18.21% and 7.39%, respectively, with E. caucasicum yielding a higher percentage of oil. Stigmastanol (21.35%) and β-sitosterol (12.19%) constituted the predominant sterols in E. caucasicum and C. platycarpos, respectively.

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