بررسی مقایسه‌ای اثر ضدباکتریایی پالیده لاکتوباسیلوس اسیدوفیلوس، آنتی‌بیوتیک سفالکسین و اسانس سیر (Allium sativum) بر باکتری‌های غالب ایجاد‌کننده اوتیت در سگ‌های شهرستان ارومیّه
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
محمدحسین صادقی زالی
1
1 - استادیار گروه میکروبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
تاریخ دریافت : 1401/11/28
تاریخ پذیرش : 1402/05/10
تاریخ انتشار : 1402/05/01
کلید واژه:
شهرستان ارومیه,
لاکتوباسیلوس اسیدوفیلوس,
اسانس سیر,
سفالکسین,
اوتیت سگ,
چکیده مقاله :
مطالعه حاضر جهت مقایسه اثر بخشی پالیده لاکتوباسیلوس اسیدوفیلوس (ATCC:4356)، آنتی‌بیونیک سفالکسین و اسانس سیر بر باکتری های غالب ایجاد کننده اوتیت در سگ های شهرستان ارومیّه انجام گردید. بدین منظور نمونه گیری از موارد عفونت گوش میانی تعداد 102 قلاده سگ (50 نمونه مربوط به سگ‌های جنس نر و 52 نمونه مربوط به سگ های جنس ماده) انجام شد. نتایج نشان داد که از 90 نمونه، به عنوان اوتیت های باکتریائی عفونی (24/88 درصد موارد)، به ترتیب استافیلوکوکوس اورئوس (5/22 درصد)، استرپتوکوکوس پنومونیه (6/18 درصد) و سودوموناس آئروژینوزا (7/14 درصد) جدا گردید. 12 نمونه (76/11 درصد موارد) هم مربوط به اوتیت های غیر عفونی بود. همچنین مشخص گردید که پالیده لاکتوباسیلوس اسیدوفیلوس و اسانس سیر توانستند رشد باکتری های غالب ایجاد کننده اوتیت در سگ های شهرستان ارومیّه را تقریباً به اندازه آنتی‌بیونیک سفالکسین، مهار نمایند که در این بین ممانعت از رشد استافیلوکوکوس اورئوس نسبت به استرپتوکوکوس پنومونیه به طور معنی داری، بیشتر مشاهده گردید (05/0p<). همچنین میانگین حداقل غلظت بازدارندگی از رشد و حداقل غلظت کشندگی در مورد باکتری استافیلوکوکوس اورئوس تحت تاثیر پالیده لاکتوباسیلوس اسیدوفیلوس، اسانس سیر، آنتی بیوتیک سفالکسین و همچنین ترکیب پالیده لاکتوباسیلوس اسیدوفیلوس با اسانس سیر و نیز پالیده لاکتوباسیلوس اسیدوفیلوس با آنتی‌بیونیک سفالکسین، به طور غیر معنی داری کمتر از فاکتورهای مذکور، در مورد استرپتوکوکوس پنومونیه بود. همچنین حداقل غلظت بازدارندگی از رشد و غلظت باکتری کشی ترکیب اسانس سیر و آنتی‌بیوتیک سفالکسین بر روی هر دو باکتری مذکور، یکسان بود.
چکیده انگلیسی:
This study compared the effectiveness of filters of acidophilus, cephalexin, and garlic essential oil on dominant bacteria causing otitis dogs in Urmia. Garlic oil was obtained in pure form. Different dilutions of antibiotic cephalexin were prepared after preparing. The acidophilus filter preparation was carried out. Analyzing the antimicrobial properties and stability of acidophilus filter is done through determining MIC, MBC and hard Disc. The results showed that 50 cases of samples were male and 52 samples were female of 102 middle ear infection samples. In 90 cases (88.24%) the infection was due to bacterial otitis of which Staphylococcus aureus (22.5%), Streptococcus pneumoniae (18.6%) and Pseudomonas aeruginosa (14.7%) had maximum middle ear- infection leading bacteria, respectively, and 12 (11.76%) had infectious causes. Acidophilus filter and garlic oil could inhibit otitis predominant bacteria (Staphylococcus aureus and Streptococcus pneumoniae) in dog in the city of Urmia almost as common antibiotics and inhibited the growth of Staphylococcus aureus significantly more than Streptococcus pneumoniae (P <0.05). The mean minimum inhibitory concentrations and bactericidal concentration of Staphylococcus aureus growth affected by acidophilus filter, garlic oil and cephalexin antibiotic and also the complexes of garlic essential oil + acidophilus filter, and acidophilus filter + cephalexin were significantly lower than that of Streptococcus pneumonia and the minimum inhibitory concentration and bactericidal concentration of the growth of garlic oil + cephalexin was the same on both of bacteria.
منابع و مأخذ:
Akimoto, Y., Uda, A., Omata, H., Shibutani, J., Nishimura, H., Komiya, M., et al. (1990). Cephalexin concentrations in human serum, gingiva, and mandibular bone following a single oral administration. General Pharmacology, 21(5): 621-623.
Alberti, P.W., Bastos, I., Bluestone, C.D., Brobby, G. and Hendarmin, H. (2000). Prevention of hearing impairment from chronic otitis media. Report of a Who/Ciba Foundation Worksho, pp: 24-25.
Allium sativum, L. Kew science (2017). Plants of the World Online; Royal Botanic Gardens, Kew, England.
García Perdomo, C.M., Ramírez Minota, P.A., Zúñiga-Benítez, H. and PeñuelaA. (2022). Cephalexin removal by persulfate activation using simulated sunlight and ferrous ions. Water Science Technology, 85(1): 52-62.
Ghadimipour, R., Sedigh-Eteghad, S., Chalangar, R., Alipoor-yeganeh, M., Khadiri, B. and Afkari Gh. (2015). In Vitro antibacterial properties of aqueous extract of garlic against on common diarrhea causing bacteria. Journal of North Khorasan University of Medical Sciences, 7(2): 357-366. [In Persian]
Kefili, T., Razavi, S.H., Emam-Jome, Z., Salehi, G.R. and Neghavi, M.R. (2010). A Comparison of Two Molecular Microbiological Methods, RAPD-PCR and DGGE-PCR for Identification of Lactobacilli Strains Isolated During Lighvan Cheese Making Process. Iranian Journal of Biosystems Engineering, 40: 35-45. [In Persian]
Khiralla, G.M., Mohamad, E.A.H., Farag, A.G. and Elhariry, H. (2015). Ntibiofilm effect of Lactobacillus pentosus and Lactobacillus plantarum cell-free supernatants against some bacterial pathogens. Journal of Biotechnology Research, 6: 86-95.
Kullen, M.J., Sanozky-Dawes, R.B., Crowell, D.C. and Klaenhammer, T.R. (2002). Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. Journal of Applied Microbiology, 89(2): 511-516.
Lieberthal, A.S., Carroll, A.E., Chonmaitree, T. Ganiats, T.G., Hoberman, A., Jackson, M.A., et al. (2013). The diagnosis and management of acute otitis media. Pediatrics, 131(3): 964-999.
Namaei, M.H., Mehramiz, M., Ghannadkafi, M. and Mofatteh, M.R. (2015). Prevalence of bacterial causes of chronic otitis media with effusion and their drug resistance in Birjand. Journal of Birjand University of Medical Sciences, 22(1): 59-66. [In Persian]
Minovi, A. and Dazert, S. (2014). Diseases of the middle ear in childhood". GMS current topics in otorhinolaryngology. head and neck surgery, 13: Doc11. doi: 10.3205/cto000114. PMC 4273172 Freely accessible. PMID 25587371.
Qureishi, A., Lee, Y., Belfield, K., Birchall, J.P. and Daniel, M. (2014). Update on otitis media - prevention and treatment. Infection and Drug Resistance, 10: 15-24.
Sambanthamoorthy, K., Feng, X., Patel, R., Patel, S. and Paranavitana, C. (2014). Antimicrobial and antibiofilm potential of biosurfactants isolated from Lactobacilli against multi –drug –resistant patogens. BMC Microbiology, 14: 1-9.
Saki, N., Nikakhalagh, S., Sarafraz M., Rahim, F. and Zarpeyma, S. (2010). Epidemiological study of otitis media in children aged less than 6 years referring to health centers of Hovaiezeh city, Scientific Medical Journal (AJUMS), 9(1): 53-62. [In Persian]
Srinivasan, K. (2009). Dietary fenugreek seed regressespreestalished cholesterol gallstones in mice. Canadian Journal of Physiology and Pharmacology, 87(9): 684-693.
Verhoeff, M., Vanderveen, E.L., Rovers, M.M., Sanders, E.A.M. and Schilder A.G.M. (2006). Chronic suppurative otitis media. International Journal of Pediatric Otorhinolaryngology, 70(1): 1-2.
Yousefvand, E., Safinejad, K. and Mohammad Asghari H. (2018). Comparing Antibacterial effects of Garlic with Antibiotics by Staphylococcus Aureus Resistant to Methicillin, Streptococcus Pneumoniae and Shigella Dysenteriae Bacteria. New Cellular and Molecular Biotechnology Journal, 8(30): 53-60. [In Persian]
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Akimoto, Y., Uda, A., Omata, H., Shibutani, J., Nishimura, H., Komiya, M., et al. (1990). Cephalexin concentrations in human serum, gingiva, and mandibular bone following a single oral administration. General Pharmacology, 21(5): 621-623.
Alberti, P.W., Bastos, I., Bluestone, C.D., Brobby, G. and Hendarmin, H. (2000). Prevention of hearing impairment from chronic otitis media. Report of a Who/Ciba Foundation Worksho, pp: 24-25.
Allium sativum, L. Kew science (2017). Plants of the World Online; Royal Botanic Gardens, Kew, England.
García Perdomo, C.M., Ramírez Minota, P.A., Zúñiga-Benítez, H. and PeñuelaA. (2022). Cephalexin removal by persulfate activation using simulated sunlight and ferrous ions. Water Science Technology, 85(1): 52-62.
Ghadimipour, R., Sedigh-Eteghad, S., Chalangar, R., Alipoor-yeganeh, M., Khadiri, B. and Afkari Gh. (2015). In Vitro antibacterial properties of aqueous extract of garlic against on common diarrhea causing bacteria. Journal of North Khorasan University of Medical Sciences, 7(2): 357-366. [In Persian]
Kefili, T., Razavi, S.H., Emam-Jome, Z., Salehi, G.R. and Neghavi, M.R. (2010). A Comparison of Two Molecular Microbiological Methods, RAPD-PCR and DGGE-PCR for Identification of Lactobacilli Strains Isolated During Lighvan Cheese Making Process. Iranian Journal of Biosystems Engineering, 40: 35-45. [In Persian]
Khiralla, G.M., Mohamad, E.A.H., Farag, A.G. and Elhariry, H. (2015). Ntibiofilm effect of Lactobacillus pentosus and Lactobacillus plantarum cell-free supernatants against some bacterial pathogens. Journal of Biotechnology Research, 6: 86-95.
Kullen, M.J., Sanozky-Dawes, R.B., Crowell, D.C. and Klaenhammer, T.R. (2002). Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. Journal of Applied Microbiology, 89(2): 511-516.
Lieberthal, A.S., Carroll, A.E., Chonmaitree, T. Ganiats, T.G., Hoberman, A., Jackson, M.A., et al. (2013). The diagnosis and management of acute otitis media. Pediatrics, 131(3): 964-999.
Namaei, M.H., Mehramiz, M., Ghannadkafi, M. and Mofatteh, M.R. (2015). Prevalence of bacterial causes of chronic otitis media with effusion and their drug resistance in Birjand. Journal of Birjand University of Medical Sciences, 22(1): 59-66. [In Persian]
Minovi, A. and Dazert, S. (2014). Diseases of the middle ear in childhood". GMS current topics in otorhinolaryngology. head and neck surgery, 13: Doc11. doi: 10.3205/cto000114. PMC 4273172 Freely accessible. PMID 25587371.
Qureishi, A., Lee, Y., Belfield, K., Birchall, J.P. and Daniel, M. (2014). Update on otitis media - prevention and treatment. Infection and Drug Resistance, 10: 15-24.
Sambanthamoorthy, K., Feng, X., Patel, R., Patel, S. and Paranavitana, C. (2014). Antimicrobial and antibiofilm potential of biosurfactants isolated from Lactobacilli against multi –drug –resistant patogens. BMC Microbiology, 14: 1-9.
Saki, N., Nikakhalagh, S., Sarafraz M., Rahim, F. and Zarpeyma, S. (2010). Epidemiological study of otitis media in children aged less than 6 years referring to health centers of Hovaiezeh city, Scientific Medical Journal (AJUMS), 9(1): 53-62. [In Persian]
Srinivasan, K. (2009). Dietary fenugreek seed regressespreestalished cholesterol gallstones in mice. Canadian Journal of Physiology and Pharmacology, 87(9): 684-693.
Verhoeff, M., Vanderveen, E.L., Rovers, M.M., Sanders, E.A.M. and Schilder A.G.M. (2006). Chronic suppurative otitis media. International Journal of Pediatric Otorhinolaryngology, 70(1): 1-2.
Yousefvand, E., Safinejad, K. and Mohammad Asghari H. (2018). Comparing Antibacterial effects of Garlic with Antibiotics by Staphylococcus Aureus Resistant to Methicillin, Streptococcus Pneumoniae and Shigella Dysenteriae Bacteria. New Cellular and Molecular Biotechnology Journal, 8(30): 53-60. [In Persian]
