اثر آستاگزانتین بر آسیب القاءشده توسط کلرید کادمیوم در بیضهء موش‌صحرایی نر بالغ نژاد ویستار
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکیمهوش حافظ‌امینی 1 , اکرم عیدی 2 , سید پژمان مرتضوی 3 , فتاح ستوده نژاد نعمت الهی 4
1 - دانش‌آموخته دکتری تخصصی فیزیولوژی، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - استاد گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - دانشیار گروه پاتولوژی، دانشکده دامپزشکی، واحدعلوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
4 - استادیار گروه زیست شناسی، واحدعلوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: آستاگزانتین, موش صحرایی, بیضه, آسیب, کلرید کادمیوم,
چکیده مقاله :
نقش حفاظتی آستاگزانتین بر سلامتی انسان، از جمله پیشگیری از بیماری‌های قلبی- عروقی و جلوگیری از روند پیرشدن سلول‌ها، اشاره به خاصیت آنتی‌اکسیدانی بسیار قوی این ماده دارد. کادمیوم یکی از آلاینده‌های موجود در اکوسیستم ها است. این فلز سنگین به عنوان یک توکسین بر سیستم تولیدمثل اثر گذاشته و سبب تخریب روند اسپرماتوژنز در بافت بیضه می شود. هدف مطالعه حاضر، بررسی تأثیر آستاگزانتین بر آسیب بیضه ای القاءشده توسط کلرید کادمیوم در موش صحرایی بود. بدین منظور، تعداد ۴۵ سر موش صحرایی نر بالغ نژاد ویستار به صورت تصادفی به ۹ گروه تقسیم شدند. گروه کنترل سالم هیچ گونه تیماری دریافت نکرد. گروه شاهد، سرم فیزیولوژی، گروه کنترل نابارور، کلرید کادمیوم (به میزان 1 میلی گرم بر کیلوگرم وزن) به صورت تزریق درون صفاقی، 3 گروه آستاگزانتین را با دوزهای 5، ۱۰ و ۲۰ میلی گرم بر کیلوگرم وزن و 3 گروه هم همزمان کلرید کادمیوم و آستاگزانتین را با دوزهای 5، ۱۰ و ۲۰ میلی گرم بر کیلوگرم به صورت گاواژ دریافت کردند. از هموژنات بافت بیضه جهت سنجش پارامترهای استرس اکسیداتیو، به عنوان نمونه‌های آزمون استفاده شد. پارامترهای مورد نظر به روش فتومتریک با دستگاه الایزا ریدر و میکروپلیت ریدر بررسی شد. آستاگزانتین با مقادیر 10 و 20 میلی‌گرم بر کیلوگرم وزن، موجب افزایش آنزیم های آنتی اکسیدانی سوپراکسید دیسموتاز، گلوتاتیون پراکسیداز و کاتالاز و همچنین کاهش میزان مالون‌دی‌آلدئید شد (001/0p). بنابراین، آستاگزانتین احتمالاً به عنوان یک آنتی اکسیدان در محافظت از بیضه موش صحرایی در برابر استرس اکسیداتیو ناشی از کلرید کادمیوم عمل می کند.
چکیده انگلیسی:
The protective role of astaxanthin on human health, including the prevention of cardiovascular disease, prevention of cell aging, refers to the very strong antioxidant properties of this substance. Cadmium is one of the pollutants in ecosystems. This heavy metal acts as a toxin on the reproductive system and destroys the process of spermatogenesis in testicular tissue. The aim of this study was to evaluate the effect of astaxanthin on cadmium chloride induced testicular damage in adult male Wistar rats. In this study, 45 rats were randomly divided into 9 groups. The healthy control group did not receive any treatment. Control group, physiological serum, infertile control group, cadmium chloride (1 mg / kg body weight) by intraperitoneal injection, astaxanthin receiving groups (5, 10 and 20 mg / kg body weight) by gavage and groups They received cadmium chloride and astaxanthin (5, 10 and 20 mg / kg) at the same time. Test samples from testicular tissue homogenates were used to measure oxidative stress parameters. The parameters were analyzed by photometric method with ELISA reader and microplate reader. The results showed that astaxanthin (10 and 20 mg / kg body weight) increased the antioxidant enzymes SOD, GPX and CAT and astaxanthin (10 mg / kg body weight) decreased MDA. Thus, astaxanthin may act as an antioxidant in protecting the rat testis against oxidative stress induced by cadmium chloride.
منابع و مأخذ:
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Ahmadi, Z. and Ashrafizadeh, M. (2018). Downregulation of osteocalcin gene in chickens treated with lead acetate II. International Biological and Biomedical Journal, 4(4): 177-182.
Acosta, I.B., Junior, A.S.V., EF, E.S., Cardoso, T.F., Caldas, J.S., Jardim, R.D., et al. (2016). Effects of exposure to cadmium in sperm cells of zebrafish. Toxicology Reports, 3(1): 696-700.
Agarwal, A., Prabakaran, S. and Said, T. (2005). Prevention of oxidative stress injury to sperm. Journal of Andrology, 26(6): 654-660.
Agarwal, A., Virk, G., Ong, C. and Du Plessis, S. (2014). Effect of oxidative stress on male The World Journal of Men's Health, 32(1): 1-17.
Aitken, R.J. (1997). Molecular mechanisms regulating human sperm function. Molecular Human Reproduction, 3(3): 169-173.
Andrisani, A., Dona, G., Tibaldi, E., Maria Brunati, A., Sabbadin, C.H., Armanini, D., et al. (2015). Astaxanthin improves human sperm capacitation by inducing lyn displacement and activation. Marine Drugs, 13(9): 5533-5551.
Alkhedaide, A., Alshehri, Z.S., Sabry, A., Abdel-Ghaffar, T., Soliman, M.M. and Attia. H. (2016). Protective effect of grape seed extract against cadmium-induced testicular dysfunction. Molecular Medicine Reports, 13(4): 3101-3109.
Alvarez, J.G., Touchtone, J., Touchstone J.C., Blasco, L. and Storey, B. (1987). Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa superoxide dismutase as major enzyme protectant against oxygen toxicity. Journal of Andrology, 8(5): 338-348.
Amara, S., Abdelmelek, H., Garrel, C., Guiraud, P., Douki, T., Ravanat, J.L., et al. (2008). Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. Development & Reproduction, 54(2): 129-134.
Aprioku, J.S., (2013). Pharmacology of free radicals and the impact of reactive oxygen species on the testis. Journal of Reproduction and Infertility, 14(4): 158-172.
Asadi, N. (2017). The impact of oxidative stress on testicular function and the role of antioxidants in improving it: a review. Journal of Clinical and Diagnostic Research: JCDR, 11(5): 1-5.
Asghari, M.H., Moloudizargari, M., Bahadar, H. and Abdollahi, M. (2016). A review of the protective effect of melatonin in pesticide-induced toxicity. Expert Opinion on Drug Metabolism and Toxicology, 13(5): 545-554.
Ashrafizadeh, M. and Ahmadi, Z. (2019). Effect of Astaxanthin treatment on the sperm quality of the mice treated with nicotine. Reviews in Clinical Medicine, 6(1): 1-5.
Bahmanzadeh, M., Vahidnia, A., Mahdinejadiani, S.H., Shokri, S. and Alizadeh, Z. (2016). Dietary supplementation with astaxanthin may ameliorate sperm parameters and DNA integrity in streptozotocin-induced diabetic rats. Clinical and Experimental Reproductive Medicine, 43(2): 90-96.
Baninameh, Z., Moloudizargari, M., Ghasemnejad Berenji, H., Rezaie Chamani, S., Hassanzadeh, V., Ferrante, M., et al. (2016). Urtica dioica attenuate effect of doxorubicin-induced changes on sperm parameters in the International Journal of Medical Research and Health Sciences, 5(11): 116-121.
Bauer, R., Demeter, I., Hasemann, V. and Johansen, J.T. (1980). Structural properties of the zinc site in Cu,Znsuperoxide dismutase; perturbed angular correlation of gamma ray spectroscopy on the Cu, 111Cd-superoxide dismutase derivative. Biochemical and Biophysical Research Communications, 94(4): 1296-1302.
Borowitzka, M.A. (1999). Commercial production of microalgae: ponds, tanks, tubes and fermentors. Journal of Biotechnology, 70(1): 313-321.
Caslino, E., Sblano, C. and Landriscina, C. (1997). Enzyme activity alteration by cadmium administration to rats: the possibility of iron involuement in lipid peroxidation. Archives of Biochemistry and Biophysics, 346(2): 171-179.
Dhankhar, J., Kadiad, S. and Sharma, A. (2012). Astaxanthin: A potential carotenoid. International Journal of Pharmaceutical Sciences and Research, 3(5): 1246.
Dokumacioglu, E., Iskender, H., Yenice, , Kapakin, K.A.T., Sevim, C., Hayirli, A., et al. (2018). Effects of astaxanthin on biochemical and histopathological parameters related to oxidative stress on testes of rats on high fructose regime. Andrologia, 50(7): 1-10.
Farhangdoost, F., JafariBarmak, M., Hemayatkhah Jahromi, V., Azizi, A., Mahmoodi, R., et al. (2013). Aloe vera extract effect on sperm quality and testicular tissue of rats induced by cadmium chloride. Armaghane-Danesh, 19(1): 47-55.
Fatahian Dehkordi, R., Alijani, M.R., Rahimi Junqani, R. and Bahadoran, Sh. (2020). The effects of pomegranate skin extract and powder on renal tissue structure following experimental cadmium poisoning in Japanese quail. Veterinary Clinical Pathology, 14(53): 37-50. [In Persian]
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