اثر تمرین تناوبی با شدت متوسط بر نشانگرهای بیوژنز میتوکندری موشهای تغذیه شده با رژیم غذایی پر چرب
محورهای موضوعی : فعالیت بدنی و تندرستی
احمد خواجوند عابدینی
1
,
احمد عبدی
2
,
علیرضا براری
3
,
قاسم ترابی پلت کله
4
,
مصطفی کاظمی
5
,
معصومه السادات میرشفائی
6
1 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
3 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
4 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
5 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
6 - کارشناس ارشد گروه فیزیولوژی ورزشی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران
کلید واژه:
چکیده مقاله :
گزارش شده که PGC-1α با همکاری Nrf2 نقش اساسی در بیوژنز میتوکندری عضلات اسکلتی دارد. هدف از پژوهش حاضر بررسی اثر تمرین تناوبی با شدت متوسط بر نشانگرهای بیوژنز میتوکندری موشهای تغذیه شده با رژیم غذایی پر چرب بود. در این مطالعه تجربی، 24 موش صحرایی نر به طور تصادفی به سه گروه (8=n): رژیم غذایی نرمال (ND)، رژیم غذایی پرچرب (HFD) و رژیم غذایی پرچرب+تمرین (HFDT) تقسیم شدند. گروه تمرین به مدت 8 هفته و 5 جلسه در هفته بر روی نوار گردان تمرین تناوبی با شدت متوسط (MIIT) را انجام دادند. برنامه MIIT شامل 13 وهله فعالیت 4 دقیقهای با شدت 25-16 متر در دقیقه، و با دورههای استراحتی 2 دقیقهای بود. دادهها با استفاده از ANOVA در سطح معنیداری 05/0 p< آزمون شد. میزان بیان نسبی PGC-1α (0001/0=p) و Nrf2 (0001/0=p) در گروههای HFD نسبت به CN کاهش معنیداری داشت. همچنین میزان بیان نسبی PGC-1α (0001/0=p) و Nrf2 (0001/0=p) در گروههای HFDT نسبت به HFD افزایش معنیداری داشت. به نظر MIIT با افزایش بیان PGC-1α و Nrf2، یک روش مناسب برای بهبود عملکرد بیوژنز میتوکندری موشهای تغذیه شده با HFD میباشد.
PGC-1α has been reported to play an essential role in skeletal muscle mitochondrial biogenesis in collaboration with Nrf2. The aim of the present study was to investigate the effect of moderate intensity interval training on mitochondrial biogenesis markers in mice fed a high fat diet. In this experimental study, 24 male rats were randomly divided into three groups (n=9): Normal Diet (ND), High-Fat Diet (HFD) and High-Fat Diet +Training (HFDT). The training group performed moderate intensity interval training (MIIT) on the treadmill for 8 weeks and 5 sessions per week. The MIIT program includes 13 repetitions of 4-minute activity with an intensity of 16-25 meters per minute, with 2-minute rest periods. Data were analyzed using ANOVA at p<0.05. There was a significant decrease in the relative expression of PGC-1α (p=0.0001) and Nrf2 (p=0.0001) in HFD groups compared to CN. Also, the relative expression of PGC-1α (p=0.0001) and Nrf2 (p=0.0001) increased significantly in HFDT groups compared to HFD. It seems that MIIT by increasing the expression of PGC-1α and Nrf2 is a suitable method to improve the performance of mitochondrial biogenesis in HFD-fed rats.
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