کارایی عصاره بادیان رومی (Pimpinella anisum)روی فعالیت زیستی Tribolium castaneum و بررسی عملکرد آنتی اکسیدانی آن

محورهای موضوعی : گیاه پزشکی

شهلا امینی ¹ ()
پریسا جنوبی ² ()
عارف معروف ³ ()
احمد مجد ⁴ ()

تاریخ دریافت : 1394/04/01 تاریخ پذیرش : 1394/09/04 تاریخ انتشار : 1393/09/01

کلید واژه: Antioxidant, شپشه آرد, Tribolium castaneum, Pimpinella anisum, آفات انباری, Biological activity, stored product pests, Apiaceae, بادیان رومی, فعالیت زیستی, تیره چتریان, آنتی‎اکسیدان,

چکیده مقاله :

شپشه آرد Tribolium castaneumیکی ازآفات مهم محصولات انباری می‎باشد، برای کنترل این آفات عصاره‎های گیاهی جایگزین مناسبی برای سموم شیمیایی قلمداد می‎شوند. از سوی دیگر گیاهان دارویی به عنوان منابع طبیعی که دارای خاصیت آنتی‎اکسیدانی هستند، مورد توجه محققین برای استفاده در سامانه‎های غذایی و بیولوژیک قرار گرفته‎اند. این تحقیق به منظور بررسی کارایی عصاره بادیان رومی (Pimpinella anisum)روی فعالیت زیستی شپشه آرد و همچنین بررسی عملکرد آنتی‎اکسیدانی این گیاه می‎باشد. در این پژوهش اثر عصاره بذر بادیان رومی روی شاخص‎های تغذیه‎ای حشرات کامل شپشه آرد، درسه غلظت 50، 75 و 100 میکرولیتر و اثرات سمیت تنفسی عصاره نیز (با غلظت‎های 50، 89، 158، 281 و 500 میکرولیتر بر لیتر هوا) در شرایط آزمایشگاهی (دمای 1±29 درجه سلسیوس و رطوبت نسبی 5±65 درصد) انجام شد. همچنین خواص آنتی‎اکسیدانی بادیان رومی با استفاده از دو روش مهار رادیکال پایدار 2و2 دی فنیل-1- پیکریل هیدرازیل (DPPH) و قدرت احیاکنندگی آهن مورد بررسی قرار گرفت. بررسی شاخص‌های تغذیه‎ای نشان داد که بالاترین و پایین ‌ترین غلظت عصاره به ترتیب 86/94 و 68/82 درصد، بازدارندگی تغذیه‎ای داشت. همچنین بیشترین مقدار تلفات در غلظت 500 میکرولیتر بر لیتر هوا و در زمان 24 ساعت ثبت گردید. غلظت لازم برای مرگ و میر 50 درصد جمعیت (LC50) در زمان 24 ساعت پس از تیمار، 19/215 میکرولیتر بر لیتر هوا ثبت شد. در آزمون DPPH، عصاره برگی، غلظت مهار 50 درصد (IC50) بالاتری نسبت به بذر نشان داد، در آزمون احیاکنندگی آهن نیز نتیجه مشابهی بدست آمد.

چکیده انگلیسی:

The red flour beetles Tribolium castaneum is one of the most important pests of stored products, to control this pests plant extracts are suitable components as alternate for chemical pesticides. On the other hand herbs as natural resources that are antioxidant properties, The attention of researchers for use in the food and biological systems are located. In this research theefficiency of Pimpinella anisum extract on biological activity of anise Tribolium castaneum and its antioxidant functional is evaluated. In this study, the effect of seed extract of anisum on nutritional indices of adults T.castaneum, in 3 concentrations 50, 75 and 100 µl and respiratory toxicity effects of extracts ( 50, 89, 158, 281, 500 µl of extract per liter of air) was in vitro (29 ± 1°C and 65 ± 5% R.H.). Also the antioxidant effects of  P.anisum was investigated by two methods, radical scavenging, DPPH  (2, 2-diphenyl-1-picrylhydrazyl) method and reducing power of iron method. Nutrition a lindices showed that the highest and lowest concentration, respectively, has 86/94% and 68/82% inhibition of feeding. The highest mortality concentration was 500 µl/L of air at 24 hours. The required concentration to 50% mortality of population (LC50) at 24 hours after treatment was 215/19 µl/L of air. In DPPH test, the 50% inhibitory concentration of leaf extract was higher than seed extract, the same result was obtained from the power reducing of iron test.

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