اثر هشت هفته تمرین استقامتی فزاینده و مکمل مرزنجوش بر شاخص‌های التهابی در موش‌های صحرایی سالم
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستیصمد امینی فر 1 , محمد نریمانی راد 2 , حمید معین 3
1 - دانشگاه
2 - گروه فیزیولوژی
3 - گروه فیزیولوژی
کلید واژه: تمرین فزاینده, التهاب, آسیب عضلانی, مکمل گیاهی مرزنجوش,
چکیده مقاله :
ز زمینه و هدف: التهاب عضلانی یا میوزیت یک توصیف کلی برای بروز التهابات مزمن و پی شرونده عضلات است. هدف از پژوهش حاضر بررسی اثر هشت هفته تمرین استقامتی فزاینده به همراه مکمل مرزنجوش بر شاخص های التهابی عضلانی در موش های صحرایی سالم بود. روش کار: در این مطالعه 27 سر موش صحرایی حدود دو ماه(250-200 گرم) به صورت تصادفی در سه گروه کنترل، تمرین استقامتی و تمرین استقامتی + مرزنجوش قرار گرفتند. برنامه تمرین استقامتی روی تردمیل به مدت هشت هفته، پنج روز در هفته، بدون شیب و هفته اول تا سوم هر جلسه به مدت 40 دقیقه و در هفته هشتم به مدت 60 دقیقه اجرا شد. مکمل مرزنجوش با استفاده از مخلوط آب به موش های گروه مکمل داده شد. یافته ها بر اساس آمار توصیفی میانگین و انحراف استاندارد و آمار استنباطی تجزیه و تحلیل واریانس یک راهه در سطح معنی داری(05/0=p) با spss تجزیه تحلیل شد. یافته ها: نتایج نشان داد اختلاف معناداری بین سه گروه در میزان TNFα,IL-6, CK وجود دارد. نتایج آزمون تعقیبی توکی نشان داد میزان شاخص های التهابی بعداز فعالیت ورزشی بدون مکمل افزایش معناداری دارد، که در گروه استقامتی + مرزنجوش به طور معنی داری پایین تر از گروه کنترل بود. هم چنین، نتایج نشان داد اختلافی بین گروه تمرین استقامتی و استقامتی با مرزنجوش و گروه کنترل وجود دارد. نتیجه گیری: تمرین استقامتی فزاینده همراه مصرف مرزنجوش احتمالاً به خاطر وجود ترکیبات ضد اکسایشی سبب افزایش ظرفیت تام اکسیدانی و تعادل واکنش های ردوکس در مقابل رادیکال های آزاد تولیدی در اثر فعالیت بدنی شدید می شود.
Background and Aims: Muscle inflammation or myositis is a general description of chronic and progressive inflammation involving muscles. The aim of this study was to investigate the effect of eight weeks of increased training with marjoram supplementation on muscle inflammatory indices in healthy rats. Methods: In this study, 27 rats of about two months (200-250 g) were randomly divided into three groups: control, endurance training and endurance training + marjoram. They got rat were kept in groups of 9 in conditions of free access to water and food. The treadmill endurance training program was performed for eight weeks, five days a week, without slope, and for the first to third week of each session for 40 minutes, and in the eighth week for 60 minutes. Marjoram supplement was given to the rat in the supplement group using a mixture of water. Findings were analyzed spss at the significance level (p = 0.05). Findings: The results showed that there was a significant difference between the three groups in the amount of TNFα, IL-6, CK. The results of Tukey post hoc test showed that the rate of inflammatory markers after exercise without supplementation increased significantly, which was significantly lower in the endurance + marjoram group than the control group. Also, the results showed that there was a difference between the endurance and endurance training groups with marjoram and the control group. Conclusion: Increased endurance training with marjoram consumption probably increases the total oxidative capacity.
1.Abidov, M., Ramazanov, Z., Seifulla, R., Grachev, S. (2019). The effects of Xanthigen™ in the weight management of obese premenopausal women with non‐alcoholic fatty liver disease and normal liver fat. Diabetes, Obesity and Metabolism, 12(1);72-81.
2.Adams, J. (2005). Oregano and marjoram: an herb society of america guide to the genus origanum. The Herb Society of America Press. kirtland USA, 4; 7-52.
3.Adesanoye, OA., Farombi, EO. (2018). Hepatoprotective effects of Vernonia amygdalina (astereaceae) in rats treated with carbon tetrachloride. Experimental Toxicological Pathology, 62(2); 197-206.
4.Ahmad, A., Pillai, K., Najmi, AK., Ahmad, SJ., Pal, S., Balani, D. (2015). Evaluation of hepatoprotective potential of jigrine post-treatment against thioacetamide induced hepatic damage. Journal of Ethnopharmacology, 79(1); 35-41.
5.Aksu, I., Topcu, A., Camsari, UM., Acikgoz, O. (20200. Effect of acute and chronic exercise on oxidant–antioxidant equilibrium in rat hippocampus, prefrontal cortex and striatum. Neuroscience Letters, 452(3); 281-285.
6.Arsenault, BJ., Cote, M., Cartier, A., Lemieux, I., Despres, JP., Ross, R. (2019). Effect of exercise training on cardiometabolic risk markers among sedentary, but metabolically healthy overweight or obese post-menopausal women with elevated blood pressure. Atherosclerosis, 207(2); 530-533.
7.Arulselvan, P., Fard, MT., Tan, WS., Gothai, S., Fakurazi, S., Norhaizan, ME. (2016). Role of antioxidants and natural products in inflammation. Oxidative Medicine and Cellular Longevity, 15;112-121.
8.Balducci, S., Zanuso, S., Nicolucci, A., Fernando, F., Cavallo, S., Cardelli, P. (2017). Anti-inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nutrition, Metabolism and Cardiovascular Diseases, 20(8); 608-617.
9.Bergman, RN., Ader, M. (2016). Free fatty acids and pathogenesis of type 2 diabetes mellitus. Trends in Endocrinology & Metabolism, 11(9); 351-356.
10.Church, C., Moir, L., McMurray, F., Girard, C., Banks, GT., Teboul, L. (2019). Overexpression of Fto leads to increased food intake and results in obesity. Nature genetics, 42(12); 1086.
11.Dorneles, GP., Haddad, DO., Fagundes, VO., Vargas, BK., Kloecker, A., Peres, A. (2016). High intensity interval exercise decreases IL-8 and enhances the immunomodulatory cytokine interleukin-10 in lean and overweight–obese individuals. Cytokine, 77; 1-9.
12.Drogoudi, P.D., Tsipouridis, C., Michailidis, Z. (2015). Physical and chemical characteristics of pomegranates. HortScience, 40(5);1200-1203.
13.Fain, JN. (2016). Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cells. Vitamins & Hormones, 74; 443-477.
14.Ferrier, KE., Nestel, P., Taylor, A., Drew, BG., Kingwell, BA. (2018). Diet but not aerobic exercise training reduces skeletal muscle TNF-α in overweight humans. Diabetologia, 47(4); 630-637.
15.Fisher, G., Hyatt, TC., Hunter, GR., Oster, RA., Desmond, RA., Gower, BA. (2017). Effect of diet with and without exercise training on markers of inflammation and fat distribution in overweight women. Obesity, 19(6);1131-1136.
16.Friden, J., Lieber, RL. (2020). Eccentric exercise‐induced injuries to contractile and cytoskeletal muscle fibre components. Acta Physiologica Scandinavica, 171(3);321-326.
17.Lira, FS., Rosa, J.C., Yamashita, AS., Koyama, CH., Batista, Jr.ML., Seelaender, M. (2019). Endurance training induces depot-specific changes in IL-10/TNF-α ratio in rat adipose tissue. Cytokine, 45(2); 80-85.
18.Mogharnasi, M., Eslami, R., Behnam, B. (2014). Effects of endurance and circuit resistance trainings on lipid profile, heart rate, and hematological parameters in obese male students. Annals of Applied Sport Science, 4(2); 25-36. [Farsi].
19.Peake, JM., Suzuki, K., Hordern, M., Wilson, G., Nosaka, K., Coombes, JS. (2017). Plasma cytokine changes in relation to exercise intensity and muscle damage. European Journal of Applied Physiology, 95(5); 514-521.
20.Qu, W., Pan, Z., Ma, H. (2014). Extraction modeling and activities of antioxidants from pomegranate marc. Journal of Food Engineering, 99(1);16-23.
21.Ridker, P. M., Rifai, N., Stampfer, M. J., Hennekens, C. H. (2000). Plasma concentration of interleukin-6 and the risk of future myocardial infarction among apparently healthy men. Circulation, 101(15); 1767-1772.
22.Ropelle, ER., Flores, MB., Cintra, DE., Rocha, GZ., Pauli, JR., Morari, J. (2020). IL-6 and IL-10 anti-inflammatory activity links exercise to hypothalamic insulin and leptin sensitivity through IKKβ and ER stress inhibition. Biology, 8(8);56-68.
23.Sand, KL., Flatebo, T., Andersen, MB., Maghazachi, AA. (2018). Effects of exercise on leukocytosis and blood hemostasis in 800 healthy young females and males. World Journal of Experimental Medicine, 3(1); 11-21.
24.Santos, VC., Sierra, APR., Oliveira, R., Caçula, KG., Momesso, CM., Sato, FT. (2018). Marathon race affects neutrophil surface molecules: role of inflammatory mediators. Biochemistry, 11(12); 111-121.
25.Tanabe, Y., Maeda, S., Akazawa, N., Zempo-Miyaki, A., Choi, Y., Ra, SG. (2015). Attenuation of indirect markers of eccentric exercise-induced muscle damage by curcumin. European Journal of Applied Physiology, 115(9);1949-1957.
_||_
