بررسی بیان ژن های مرتبط با تنش در گوسفندان آمیخته و بومی DOR: 20.1001.1.17359880.1400.14.3.5.6
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
ندا روح بخش
1
(
دانشگاه محقق اردبیلی- اردبیل- ایران
)
نعمت هدایت ایوریق
2
(
عضو هیات علمی، گروه علوم دامی، دانشکده کشاورزی، دانشگاه محقق اردبیلی
)
رضا سید شریفی
3
(
عضو هیئت علمی، گروه علوم دامی، دانشکده کشاورزی، دانشگاه محقق اردبیلی
)
میرداریوش شکوری
4
(
گروه علوم دامی و صنایع غذایی، دانشکده کشاورزی و منایع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران
)
کلید واژه: گوسفند دورگ, بیان ژن, گوسفند بومی, تنش,
چکیده مقاله :
زمینه و هدف: افزایش دمای هوا و خشک شدن مراتع حیوانات را دچار تنش آبی و تغذیه ای می کند. در این پژوهش ژن‌های کنترل کننده مقاومت در گوسفندان دورگ و بومی مورد بررسی قرارگرفت.روش کار: در این تحقیق از ده بره ی نر آمیخته رومانف و بومی و ده بره نر خالص بومی در 4 تیمار و 5 تکرار در هر گروه استفاده شدکه در دوگروه با محدودیت آبی و بدون محدودیت آبی تقسیم شدند در پایان آزمایش نمونه‌ها ی بافت کبد از حیوانات کشتار شده جمع آوری و بعد از استخراج RNA و پاکسازی نمونه ها انجام واکنش Real Time PCR توالی و طول قطعه های مورد نظر بررسی قرار گرفت و برای آنالیز آماری از روش آنالیز فاکتوریل 2×2 در قالب طرح کاملاً تصادفی با استفاده از نرم افزار آماری SAS استفاده گردید.یافته ها: با استخراج ژن ها مشخص گردید میزان بیان ژن های، NRT2, PRKAA1, SIRT3, TFAM,GPX1 در شرایط تنش در گوسفندان بومی نسبت به گوسفندهای آمیخته افزایش داشته است.نتیجه گیری: مطالعات انجام یافته بر روی بیان ژن های، PRKAA1, NRT2,GPX1,TFAM SIRT3در کبد دو گروه گوسفند بومی و دورگ در شرایط تنش و نرمال چنین استنباط شد که در شرایط استرس این چهار گروه ژن با مقابله با اکسیداسیون اکسیداتیو و ذخیره انرژی و جلوگیری از اتلاف انرژی و محافظت از سلول, مقاومت حیوان را در برابر شرایط تنش افزایش می دهند با توجه به سازگاری ژنتیکی گوسفندهای بومی با شرایط بیابانی منطقه, سبب مقاومت بیشتر حیوان در برابر گرما و بیماری می گردد.
Inroduction & Objective: Increasing in temperature and drying of pastures cause water and nutritional stress to animals. In this study, resistance control genes in crossbred and native sheep were examined. Material and Methods: In this study, ten cross of Romanov native male lambs and ten purebred male native lambs in 4 treatments and 5 replications in each group were used. They are divided into two groups with normal and water restriction condition. At the end of the experiment, liver tissue samples were collected from slaughtered animals and it was kept in liquid nitrogen, to investigation of gene expression whole RNA was extracted and purified and the cDNA was synthesis. In the end Real Time PCR reaction were performed using specific primers. For statistical analysis we used randomized design as factorial analysis method using SAS 9.2. Results: By extracting the genes, it was found that the expression of GPX1, TFAM, SIRT3, PRKAA1, NRT2 genes increased under stress in native sheep compared to crossbred sheep.Conclusion: The expression of GPX1, TFAM, NRF2, SIRT, PKAA1 genes in the liver of two groups of native and crossbred in normal and stress conditions indicate that Under stress, these four groups of genes act as oxidative process and store energy and prevent energy loss and protect the cell. Due to the genetic compatibility of native sheep with the drought conditions of the region, it makes the animal more resistant to heat and disease.
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