اثرات سلول‌های بنیادی مزانشیمی ژله وارتون به همراه صمغ درخت بنه در درمان آسیب‌های پوستی ناشی از سوختگی

محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی

الهام حویزی ¹ (گروه زیست شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران)

تاریخ دریافت : 1398/05/30 تاریخ پذیرش : 1399/02/20 تاریخ انتشار : 1399/04/01

کلید واژه: صمغ درخت بنه, مطالعات بافت‌شناسی, سلول‌های بنیادی ژله وارتون, سوختگی, سلول‌درمانی,

چکیده مقاله :

زمینه‌وهدف: سوختگی یکی از آسیب‌های رایج در جهان است که می توان از روش‌های نوین مانند سلول‌درمانی و طب سنتی برای درمان آن استفاده کرد. هدف از مطالعه‌ی حاضر بررسی اثرات ضمادی از صمغ بنه و روغن حیوانی به‌همراه سلول‌های بنیادی مزانشیمی ژله وارتون در درمان سوختگی درجه سه در رت‌های نژاد ویستار می‌باشد. روش‌کار: در این آزمایش تجربی، سلول‌های بنیادی از طریق جداسازی سلول‌های ژله وارتون از بندناف انسانی حاصل شد. برای انجام این مطالعه 28 رت بواسطه استامپ فلزی سوزانده و سپس به طور تصادفی در دو گروه کنترل(7 موش) و تیمار(21 موش) تقسیم شدند. گروه تیمار به سه دسته(هر گروه 7 موش) تیماربا ضماد، سلول‌درمانی و سلول‌درمانی بعلاوه ضماد تقسیم شدند. به هر رت تعداد 106 سلول در پاساژ سه و به‌صورت زیرجلدی تزریق شد. در روز 30 بعداز درمان آسان‌کشی با کلروفروم و تهیه‌ی مقاطع بافتی با رنگ‌آمیزی هماتوکسیلسن-ائوزین و تری‌کروم‌ماسون برای بررسی‌های میکروسکوپی صورت گرفت. یافته‌ها: مشاهدات میکروسکوپی نشان داد‌که در گروه‌های تیمار روند بهبودی با سرعت بالاتری نسبت گروه کنترل انجام گرفت. هم‌چنین بعد از گذشت 30 روز روش سلول‌درمانی+ ضماد به‌طور معنی‌داری موثرتر نسبت به ضماد و تزریق سلول به‌تنهایی بود. مطالعات بافت شناسی نشان دهنده‌ی ازدیاد معنی‌دار در آنژیوژنز، سنتز کلاژن، تعداد سلول‌ها، ضخامت لایه‌های پوست و در نهایت تسریع ترمیم زخم در نمونه‌های تیمار در مقایسه با نمونه‌های گروه کنترل بود. نتیجه‌گیری: براساس نتایج به‌دست آمده، روش‌های درمانی هم‌زمان استفاده شده در این مطالعه در تسریع ترمیم زخم‌های پوستی در مدل حیوانی به‌طور معنی‌داری تاثیر گذار بوده است.

چکیده انگلیسی:

Background: Burn is a common wound in the world and consider the novel methods such as cell therapy can be a helpful strategy in the treatment. The purpose of the present study is investigating the effects of using ointment of animal oil mixed with Gum of Pistacia atlantica associated wharton's jelly mesenchymal stem cells (WJMSCs) on rat third-degree burn models. Methods:In this experimental study, WJMSCs were extracted from human umbilical cord. For this study, 28 Wistar rats were burned by heating a metal rod of 1cm in diameter and then randomly divided into the control (7 rats) and treatment (21 rats) groups. The treatment group was divided into three groups (each group of 7 rats) of daily scrubbingof ointment, cell therapy, and cell therapy+ ointment. 106 cells (passage3) were injected into each rat subcutaneously. On day 30 after treatment, animals killed by chloroform and histological sections were prepared by staining Hematoxylsene-Eosin (H&E) and Trichromosone done for microscopic study. Results: Macroscopic and microscopic results indicated that in the experimental groups, the recovery was significantly more than the control. Also, the cell therapy+ ointment was significantly more effective than ointment and cell alone after 30 days. Histological analysis demonstrated a significant increase in angiogenesis, collagen synthesis, number of cells, thickness of skin layers, and totally acceleration wound healing in experimental groups compared to controls. Conclusion: Based on these data, it can be suggested that simultaneous cell-therapy and traditional medicine accelerate the repair of skin burns in the animal models more significantly.

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