اثر لیکوپن روی پارامترهای بافت بیضه در قوچ مهربان

محورهای موضوعی : جانوری

قباد دهقانی ¹ (کارشناسی ارشد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، لرستان، ایران)
سعید محمدزاده ² (دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، لرستان، ایران)
علی کیانی ³ (دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، لرستان، ایران)

تاریخ دریافت : 1401/01/04 تاریخ پذیرش : 1401/05/06 تاریخ انتشار : 1400/10/01

کلید واژه: فراسنجه ماکروسکوپی بیضه, لیکوپن, فراسنجه میکروسکوپی بیضه, قوچ,

چکیده مقاله :

هدف: لیکوپن یک هیدروکربن آلیفاتیک است که خاصیت آنتی‌‌اکسیدانی داشته و قادر است رادیکال‌‌های آزاد را از محیط جمع‌آوری و خنثی نماید. هدف پژوهش حاضر بررسی اثر لیکوپن روی تولیدمثل قوچ نژاد مهربان است.مواد و روش‌ها: در این پژوهش 20 رأس بره نر نژاد مهربان با میانگین سن 6 ماه، وزن 5/0±27 کیلوگرم، پس از تأیید سلامت بالینی و واکسیناسیون (آنتروتوکسمی، آگالاکسی، تب برفکی) انتخاب گردیدند. دام‌‌ها به دو گروه آزمایشی 10 رأسی تقسیم شدند. پس از دو هفته دوره عادت‌‌پذیری، بره‌‌ها به مدت 100 روز با جیره‌‌هایی شامل گروه کنترل: جیره پایه، گروه آزمایشی: جیره پایه به همراه 5 گرم لیکوپن روزانه در دو نوبت صبح و عصر تغذیه شدند. آب به صورت آزاد در اختیار بره‌‌ها قرار داشت. در پایان دوره آزمایشی و پس از 24 ساعت محرومیت از خوراک، کلیه بره‌‌ها کشتار شدند. ابتدا بیضه‌ها از کیسه بیضه خارج و صفات ماکروسکوپی بیضه‌‌ها اندازه‌‌گیری شد. سپس برای مطالعات فراسنجه‌‌های میکروسکوپی، بخشی از بافت بیضه برداشته و در فرمالین 10% جهت تهیه مقاطع و مطالعه بافت بیضه تثبیت شد. داده‌‌های آزمایش با استفاده از طرح کامل تصادفی با کمک نرم‌‌افزار (2003) SAS آنالیز و مقایسه میانگین با دانکن در سطح P<0/05 درصد انجام شد.نتایج: استفاده از لیکوپن به‌‌طور معنی‌‌داری ضخامت بافت پوشش (اپیتلیوم) و قطر لومن را در لوله‌‌های اسپرم‌‌ساز نسبت به گروه کنترل، به ترتیب افزایش و کاهش داد (p˂0/05). تفاوت معنی‌‌داری در قطر لوله‌‌های اسپرم‌‌ساز و صفات ماکروسکوپی بافت بیضه در تیمارها مشاهده نشد.نتیجه‌گیری: نتایج این پژوهش نشان داد که لیکوپن تاثیر مثبتی روی لوله‌‌های اسپرم‌‌ساز بافت بیضه در قوچ دارد.

چکیده انگلیسی:

Objectives: Lycopene is an aliphatic hydrocarbon that has antioxidant properties and is capable to collect and neutralize free radicals. The purpose of this experiment was to investigate the effect of lycopene on the reproduction of Mehraban ram.Methods: In this experiment 20 male lambs aged 6 months, weighted 27±0.5 kg after clinical confirmation and vaccination (Food and mouth disease (FMD), Agalaxy and Enterotoxaemia) were selected. Lambs divided to 2 experimental groups with 10 replications. After two weeks of acclimatization, the lambs reared 100 days. The animal groups composed control (basic diet) and treatment basic diet + lycopene 5 gr/day. The lambs were fed twice per day, morning and evening. The water was freely available. At the end of the experimental period, after 24 hours of deprivation of feed, all lambs were slaughtered and testes were removed from the scrotum. Macroscopic traits were measured. Testis samples were fixed in formalin 10% for histology sections and microscopic features. Data of experiment were analyzed using a completely randomized design using SAS 2003 software and the means were compared with Duncan (P<0.05).Results: Application of lycopene significantly increased the thickness of the germinal epithelium and decreased the lumen diameter of seminiferous tubules compared to the control treatment (P˂0.05). No significant difference was observed in seminiferous tubules diameter and macroscopic traits.Conclusion: The results of this experiment showed that lycopene has positive effects on the seminiferous tubules parameters of testis in ram. 

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