مطالعه مورفولوژی و مورفومتری بیضه گربه به دنبال تجویز مقادیر مختلف بوسولفان

محورهای موضوعی : پاتوبیولوژی مقایسه ای

مهدی عصمت‌پرست، پرویز تاجیک*، حمید قاسم‌زاده‌نوا . ¹ (.)

تاریخ دریافت : 1397/01/24 تاریخ پذیرش : 1397/01/24 تاریخ انتشار : 1397/01/01

کلید واژه: بوسولفان, اسپرماتوژنز, بیضه, گربه,

چکیده مقاله :

به منظور موفقیت در پیوند سلول‌های اسپرماتوگونی نیاز است تا در اولین گام مدل مناسبی برای تولید حیوانات آزواسپرمیک تعریف گردد. روش‌های متعددی جهت تولید حیوانات آزواسپرمیک موجود است که این موارد شامل استفاده از بوسولفان به صورت سیستمیک، رادیوتراپی، ایسکمی سرمایی و افزایش دمای بیضه می‌باشند. هدف از انجام این طرح ابداع روشی جهت تولید گربه‌های آزواسپرمیک به منظور آماده‌سازی آنها برای دریافت پیوند سلول‌های بنیادی اسپرماتوگونی با تزریق بوسولفان می‌باشد. در این مطالعه از 15 راس گربه نر مو کوتاه خانگی 3 الی 5 ماهه استفاده شد. پس از اولین تزریق در هفته‌ی 5، 9، 13 و 17 دوزهای صفر، 4 و 10 میلی‌گرم/کیلوگرم وزن بدن، بوسولفان به صورت داخل وریدی به ترتیب به گربه‌های گروه‌های شاهد و تیمار تزریق شد. بیضه‌ها 2 ماه پس از پایان تزریقات بوسیله جراحی خارج شد و مورد ارزیابی قرار گرفت. همچنین میانگین قطر مجرای داخل لوله اسپرم ساز و ضخامت اپیتلیوم زاینده براساس میکرومتر محاسبه شد. سلول‌های سرتولی و سلول‌های لیدیگ نیز مورد ارزیابی قرار گرفت. در بررسی بافتی در گروه‌های تحت درمان با دوزهای 4 و 10 میلی‌گرم/کیلوگرم وزن بدن بوسولفان، درجات متفاوتی از تخریب لوله‌های اسپرم ساز، کاهش تعداد لوله‌های اسپرم ساز و ضخامت اپیتلیوم زاینده مشاهده شد. بوسولفان از طریق تأثیر بر سلول‌های زاینده و سوماتیک موجود در بیضه می‌تواند در تخریب اسپرماتوژنز و القاء آزواسپرمی در بیضه نقش داشته باشد.

چکیده انگلیسی:

In order to successfully transplant spermatogonial cells need to be defined a suitable model for producing azoospermia animals. Several methods are available for made azoospermic animals, include systemic injection of busulfan, radiation, cold ischemia and increased testicular temperature. The aim of the study is development of a method for producing azoospermic cats in order to prepare them for spermatogonial stem cells transplantation. In this study, 15 domestic short hair cats at the age of 3 to 5 month were divided in 3 groups. After first injection, at 5, 9, 13 and 17 weeks, doses of zero, four and ten mg/kg of busulfan intravenously were injected to the control and treatment groups, respectively. Two months after the injections testicles surgically removed and evaluated. The mean diameter of the seminiferous tubules and germinal epithelium thickness was calculated. Sertoli and leydig cells were also evaluated. Histological evaluation of treating groups with 4 and 10 mg/kg busulfan shows different degrees of degradation of seminiferous tubules. The thickness of germinal epithelium significantly decreased. Busulfan through its effect on germ cells and somatic cells can disrupt spermatogenesis and induced azoospermia.

منابع و مأخذ:

 

1-       Aich, S., Manna, C.K. (2001): Histophysiological changes of the testicular tissue due to busulphan administration in the wild Indian house rat. Acta. Biol. Hung. 52 (1):105-16.

2-      Anderson, P.h.O., Knoben, J.E., Troutman, W.G. (2002) Handbook of clinical data 10^th Edition. Mc Graw Hill. P: 136-140.

3-      Ballester, J., Dominguez, J., Munoz, M.C., Sensat, M., Rigau, T. (2005): Tungstate treatment improves Leydig cell function in streptozotocin- diabetic rats. J. Androl. 26(6):706-15

4-       Bar-Shira Maymon, B., Yogev, L., Marks, A., Hauser, R., Botchan, A., Yavets, H. (2004): Sertoli cell inactivation damage to the human testis after cancer chemotherapy. Fertil. Stril. 81(5):1391-1394.

5-      Boujrad, N., Hochereau-de Riviers, M.T., Kamtchouing, P., Perreau, C., Carreau, S. (1995): Evolution of somatic and germ cell populations after busulfan treatment in utero or neonatal cryptorchidism in the rat. Andrologia. 27(4):223-8.

6-      Brinster, R.L., Avarbock, M.R. (1994): Germline transmission of donor haplotype following spermatogonial transplantation. Proc. Natl. Acad. Sci. USA. 91(1): 1303-7.

 

7-      Brinster, RL., Zimmerman, JW. (1994): Spermatogenesis following male germ cell transplantation. Proc. Natl. Acad. Sci. U S A. 91(1):1298–11302.

1-            Bucci, L.R., Meistrich, M.L. (1987): Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations. Mutat. Res. 176(2):259-68

8-      Cai, L., Hales, B.F., Robaire, B. (1997): Induction of apoptosis in the germ cells of adult male rats after exposure to cyclophosphamide. Biol. Reprod. 56(6):1490-7.

9-       Choi, Y.J., Ok, D.W., Kwon, D.N., Chung, J.I., Kim, H.C. (2004): Murine male germ cell apoptosis induced by busulfan treatment correleates with loss of c-kitexpression in a Fas/FasL-and P53-independent manner. FEBS. Lett. 575(1-3):41-51.

1-             Gunderson, H.J., Bagger, P., Bendtsen, T.F., Evans, S. M, Korbo, L. (1988): The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS. 96(10):857-81.ِ

10-  Guneli, E., Tugyan, K., Ozturk, H., Gumustekin, M., Cilaker S., Uysal, N. (2008): Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur. Surg. Res. 40(4):354-60.

11-   Haddad, S., Carvalho, T.L., Anselmo-Franci, J.A., Petenusci, S.O., Favaretto, A.L. (1997): Ultrasound stimulation of rat testes damaged by busulfan. Ultrasound Med. Biol. 23(9):1421-25.

12-  Honaramooz, A., Behboodi, E., Hausler, C., Blash, S., Ayres, S., Azuma, C. (2005):  Depletion of Endogenous Germ Cells in Male Pigs and Goats in Preparation for Germ Cell Transplantation. J. Androl. 26(6):698–705.

13-  Howell, S.J., Shalet, S.M. (2005): Spermatogenesis after cancer treatment: damage and recovery. J. Natl. Cancer. Inst. Monogr. (34):12-17.

1-            Izadyar, F., Den Ouden, K., Stout, TA., Stout, J., Coret, J., Lankveld, DP., Spoormakers, TJ., Colenbrander, B. (2003): Autologous and homologous transplantation of bovine spermatogonial stem cells. Reproduc. 126(6):765-74.

14-   

 

1-            Jansz, G.F., Pomerantz, D.K. (1985): The effect of prenatal treatment with busulfan on in vitro androgen production by testes from rats of various ages. Can. J. Physio. Pharmaco. 63(9):1155-8.

15-  Jiang, F.X. (1998): Behavior of spermatogonia following recovery from busulfan treatment in the rat. Anat. Embryol. 198(1): 53-61.

16-  Jiang, FX., Short, RV. (1995): Male germ cell transplantation in rats: apparent synchronization of spermatogenesis between host and donor seminiferous epithelia. Int. J. Androl. 18(6): 326-30.

17-  Kubota, H., Brinster, RL. (2008.): Culture of rodent spermatogonial stem cells, male germline stem cells of the postnatal animal. Methods. Cell. Biol. 86: 59-84.      

18-  Kubuta, H., Avarbock, MR., Brinster, RL. (2004): Growth factors essential for self renewal and expansion of mouse spermatogonial stem cell. Proc. Natl. Acad. Sci. USA. 101(47):16489- 94.              

Lewis-Johnes, DI., Kerrigan, DD. (1985): A modified johnsen's count for evaluation of spermatogenesis in the rat. IRCS. Med. Sci.13: 510-11.

19-  Meistrich, M.L., Wilson, G., Huhtaniemi, I. (1999): Hormonal treatment after cytotoxic therapy stimulates recovery of spermatogenesis. Cancer Res. 59(15): 3557-60.

1-            Molenaar, R., de Rooij, D.G., Rommerts, F.F., Reuvers, P.J., Van der Molen, H.J. (1985): Specific destruction of Leydigcells in mature rats after in vivo administration of ethane dimethyl sulfonate. Biol. Reprod. 33(5):1213-22

20-   

21-  O' Donell, L., Robertson, K.M., Jones, M.E., Simpson, E.R. (2001): Estrogen and spermatogenesis. Endocr. Rev., 22(3):289-318

22-  Osinubi, A.A., Noronha, C.C., Okanlawon, A.O. (2005) Morphometric and stereological assessment of the effects of long-term administration of quinine on the morphology of rat testis. West. Afr. J. Med. 24(3):200-5.

23-  Petersen, P.M., Giwercman, A., Gundersen, H.J., Pakkenberg, B. (2000): Efficient and unbiased tools for quantitating laydig and sertoli cells in testes from testes biobsies. Img. Anal. Stereol. 19:113-117.

24-  Sawhney, P., Gimmona, C.J., Meistrich, M.L., Richburg, J.H. (2005): Cisplatin- induced long-term failure of spermatogenesis in adult C57/Bl/6J Mice. J. Androl. 26(1): 136-45.

25-  Shinohara, T., Orwig, KE., Avarbock, MR., Brinster, RL. (2002): Gem line stem cell competition in postnatal mouse testes. Biol. Reprod. 66:1491–1497.

26-  Shinohara, T., Orwig, KE., Avarbock, MR., Brinster, RL. (2001): Remodeling of the postnatal mouse testis is accomplished by dramatic changes in stem cell number and niche accessibility. Proc. Natl. Acad. Sci. USA. 98: 6186–6191.

27-   Shinohara, T., Orwig, K.E., Avarbock, M.R., Brinster, R.L. (2003): Restoration of spermatogenesis in infertile mice by sertoli cell transplantation. Biol. Reprod. 68(3):1064-71.

28-  Siril Ariyaratne, H.B., Ian Mason, J., Mendis- Handagama, S.M. (2000):  Effects of thyroid and luteinizing hormones on the onset of precursor cell differentiation into leydig progenitor cells in the prepubertal rat testis. Biol. Reprod. 63(3):898-904.

29-   Tae, H.J., Jang, B.G., Ahn, D.C., Choi, E.Y., Kang, H.S. (2005):  Morphometric studies on the testis of Korean ring-necked pheasant (Phasianus colchicus karpowi) during the breeding and non-breeding seasons. Vet. Res. Commun. 29(7):629-43.

30-  Tan, K.A., Walker, M., Morris, K., Greig, I., Mason, J.I., Sharpe, R.M. (2006): Infant feeding with soy formula milk: effects of puberty progression, reproductive function and testicular cell numbers in marmoset monkeys in adulthood. Hum. Reprod. 21(4): 896-904.

31-  Trevor, A.J., Katzung, B.G., Masters, S.B. (2002): A long medical book Katzung&Trevors Pharmacology examination and board review 6^th Edition. Mc Graw Hill. P: 206-8.

32-  Udagawa, K., Ogawa, T., Watanabe, T., Yumura, Y., Takeda, M., Hosaka, M. (2001):  GnRH analog, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage. Int. J. Urol. 8(11):615-22.

33-  Veccino, P., Uranga, J., Arechaga, J. (2001): Suppression of spermatogenesis for cell transplantation in adult mice. Protoplasma. 217(4):191-8.

34-  Yang, Z.W., Guo, Y., Lin, L., Wang, X.H., Tong, J.S., Zhang, G.Y. (2004): Quantitative (stereological) study of incomplete spermatogenic suppression induced by testosterone undecanoate injection in rats. Asian J. Androl. 6(4):291-7.