تاثير باكتريهاي بومي توليد كننده سيدروفور و اكسين(IAA) بر كارتنوئيد، پرولين و درصد اسانس بابونه آلماني (Matricaria Chamomilla L.)
محورهای موضوعی : گیاهان دارویی
سيد حسن تفرجي
1
,
وحید توللی
2
1 - گروه كشاورزي، دانشگاه پيام نور، تهران، ايران
2 - گروه کشاورزی-دانشگاه پیام نور-تهران-ایران
کلید واژه: اسانس, اکسین, بابونه آلمانی, باکتریهای محرک رشد, سیدروفور,
چکیده مقاله :
این پژوهش با هدف ارزیابی تأثیر باکتریهای بومی تولیدکننده سیدروفور و اکسین بر شاخصهای رشد و متابولیتهای ثانویه بابونه آلمانی (Matricaria chamomilla L.) در شرایط گلخانه انجام شد. در این مطالعه، باکتریهای ریزوسفری از خاکهای آهكي جنوب استان فارس جداسازی و از نظر تولید سیدروفور و اکسین (IAA) غربالگری شدند. جدایههای برتر با تكنيك توالي يابي ژن rRNA 16S شناسایی مولکولی شدند و تأثیر آنها در یک آزمایش گلخانهای با طرح کاملاً تصادفی با سه تکرار بر روی گیاه بابونه مورد ارزیابی قرار گرفت. شاخصها شامل وزن خشک اندام هوایی، محتوای کلروفیل کل، کاروتنوئید، پرولین و درصد اسانس بود. از بین جدایههای برتر، سویه Pseudomonas fluorescens SF1044 بهطور معنیداری باعث افزایش وزن خشک (8/22 %)، کلروفیل کل (8/35 %)، کاروتنوئید (3/35 %) و درصد اسانس (1/36 %) در مقایسه با شاهد شد. سویه Pseudomonas syringae SF1050 نیز در رتبه دوم مؤثرترین تیمارها قرار گرفت. در مقابل، سویه SF1044 موجب کاهش معنیدار محتوای پرولین (8/31 %) شد که نشاندهنده کاهش تنش در گیاهان تلقیحشده بود. نتایج گویای پتانسیل بالای جدایههای بومی، بهویژه سویه SF1044، در بهبود رشد و ارتقای کمی و کیفی بابونه از طریق مکانیسمهای مختلف است. این سویه میتواند به عنوان کاندیدی امیدوارکننده برای تولید کود زیستی و کاربرد در کشت پایدار این گیاه دارویی ارزشمند در خاکهای آهکی معرفی شود.
This research was conducted to evaluate the effect of indigenous siderophore and auxin-producing bacteria on growth indices and secondary metabolites of German chamomile (Matricaria chamomilla L.) under greenhouse conditions. In this study, rhizospheric bacteria were isolated from calcareous soils in the southern part of Fars province and screened for siderophore and auxin (IAA) production. The superior isolates were molecularly identified using 16S rRNA gene sequencing technique, and their effects were assessed in a greenhouse experiment employing a completely randomized design with three replications on chamomile plants. Measured indices included shoot dry weight, total chlorophyll content, carotenoid, proline, and essential oil percentage. Among the superior isolates, the strain Pseudomonas fluorescens SF1044 significantly increased dry weight (22.8%), total chlorophyll (35.8%), carotenoid (35.3%), and essential oil percentage (36.1%) compared to the control. The strain Pseudomonas syringae SF1050 was ranked as the second most effective treatment. In contrast, strain SF1044 caused a significant decrease in proline content (31.8%), indicating reduced stress in the inoculated plants. The results demonstrate the high potential of the indigenous isolates, particularly strain SF1044, in improving growth and enhancing the quantitative and qualitative yield of chamomile through various mechanisms. This strain can be introduced as a promising candidate for the production of biofertilizers and application in the sustainable cultivation of this valuable medicinal plant in calcareous soils.
Amalia, D. A. L., Oedjijono, O., Aziz, S., and Ardian, M. N. (2025). Exploration and Characterization of Native Rhizobacteria from Saline Soils of Brebes, Indonesia for Growth Promotion of Allium ascalonicum L. International Journal of Plant & Soil Science, 37(6): 203-214.
Asghari, B., Khademian, R., and Sedaghati, B. (2020). Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Scientia Horticulturae, 263: 109132.
Banchio, E., Bogino, P. C., Santoro, M., Torres, L., Zygadlo, J., and Giordano, W. (2010). Systemic induction of monoterpene biosynthesis in Origanum× majoricum by soil bacteria. Journal of Agricultural and Food Chemistry, 58(1) : 650-654.
Basu, A., Prasad, P., Das, S. N., Kalam, S., Sayyed, R., Reddy, M., and El Enshasy, H. (2021). Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability, 13(3) : 1140.
Batool, T., Ali, S., Seleiman, M. F., Naveed, N. H., Ali, A., Ahmed, K., . . . Alotaibi, M. (2020). Plant growth promoting rhizobacteria alleviates drought stress in potato in response to suppressive oxidative stress and antioxidant enzymes activities. Scientific Reports, 10(1): 16975.
Batovska, D., Panova, N., Gerasimova, A., Tumbarski, Y., Ivanov, I., Dincheva, I., . . . Nikolova, K. (2025). Chamomile Matters: Species-and Producer-Dependent Variation in Bulgarian Matricaria recutita L. and Chamaemelum nobile L. Essential Oils and Their Cosmetic Potential. Cosmetics, 12(3) : 123.
Dastborhan, S., Zehtab-Salmasi, S., Nasrollahzadeh, S., and Tavassoli, A. (2011). Effect of plant growth-promoting rhizobacteria and nitrogen fertilizer on yield and essential oil of German chamomile (Matricaria chamomilla L.). Paper presented at the International Symposium on Medicinal and Aromatic Plants IMAPS2010 and History of Mayan Ethnopharmacology IMAPS2011 964.
de Andrade, L. A., Santos, C. H. B., Frezarin, E. T., Sales, L. R., and Rigobelo, E. C. (2023). Plant growth-promoting rhizobacteria for sustainable agricultural production. Microorganisms, 11(4) : 1088.
El Mihyaoui, A., Esteves da Silva, J. C., Charfi, S., Candela Castillo, M. E., Lamarti, A., and Arnao, M. B. (2022). Chamomile (Matricaria chamomilla L.): a review of ethnomedicinal use, phytochemistry and pharmacological uses. Life, 12(4) : 479.
Harahap, R. T., Herdiyantoro, D., Setiawati, M. R., Azizah, I. N. R., and Simarmata, T. (2022). Potential use of PGPR based biofertilizer for improving the nutrient availability in soil and agronomic efficiency of upland rice. Kultivasi, 21(3) : 293-304.
Issifu, M., Songoro, E. K., Onguso, J., Ateka, E. M., and Ngumi, V. W. (2022). Potential of Pseudarthrobacter chlorophenolicus BF2P4-5 as a biofertilizer for the growth promotion of tomato plants. Bacteria, 1(4) : 191-206.
Johnson, J. S., Spakowicz, D. J., Hong, B.-Y., Petersen, L. M., Demkowicz, P., Chen, L., and Gerstein, M. (2019). Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nature communications, 10(1) : 5029.
Kumar, P., Rana, A., Sheokand, M., Kumar, S., Chaudhary, K., Nandal, U., and Dhaka, R. K. (2025). Biofortification and growth enhancement of wheat via bacteria-assisted iron and zinc nanoparticles. Frontiers in Nanotechnology, 7: 1595252.
Noumavo, P. A., Agbodjato, N. A., Baba-Moussa, F., Adjanohoun, A., and Baba-Moussa, L. (2016). Plant growth promoting rhizobacteria: Beneficial effects for healthy and sustainable agriculture. African Journal of Biotechnology, 15(27) : 1452-1463.
Saeed, Q., Xiukang, W., Haider, F. U., Kučerik, J., Mumtaz, M. Z., Holatko, J., and Naveed, M. (2021). Rhizosphere bacteria in plant growth promotion, biocontrol, and bioremediation of contaminated sites: a comprehensive review of effects and mechanisms. International journal of molecular sciences, 22(19) : 10529.
Santoro, M., Cappellari, L. d. R., Giordano, W., and Banchio, E. (2015). Plant growth‐promoting effects of native Pseudomonas strains on Mentha piperita (peppermint): an in vitro study. Plant Biology, 17(6) : 1218-1226.
Sarma, N., Gogoi, R., Begum, T., Lal, M., Perveen, K., Alsahikh, N. A., and Alsulami, J. A. (2023). A study on the chemical profile of cultivated chamomile (Matricaria chamomilla L.) flower essential oil from North East India with special emphasis on its pharmacological importance. Journal of Essential Oil Bearing Plants, 26(3) : 745-760.
Shamsul Hayat, S. H., Qaiser Hayat, Q. H., Alyemeni, M., Wani, A., Pichtel, J., and Aqil Ahmad, A. A. (2012). Role of proline under changing environments: a review.
Shokati, B., and Poudineh, Z. (2017). An overview of plant growth promoting rhizobacteria and their influence on essential oils of medicinal plants: a review article. Iranian Journal of Plant Physiology, 7(3) : 2051-2061.
Sridhar, D., Alherwairini, S. S., Eswaran, S. U. D., Barasarathi, J., Lalitha, S., and Sayyed, R. (2025). The soil microbiome enhances sesame growth and oil composition, and soil nutrients under saline conditions. Scientific Reports, 15(1) : 29432.
Vaghela, N., and Gohel, S. (2023). Medicinal plant‐associated rhizobacteria enhance the production of pharmaceutically important bioactive compounds under abiotic stress conditions. Journal of Basic Microbiology, 63(3-4) : 308-325.
