Comparison of the effects of acute Resistance-Aerobic training in the morning and evening on cortisol, testosterone, and insulin levels in male and female bodybuilders
محورهای موضوعی : Journal of Physical Activity and Hormones
Mahmood Padash Sotodeh
1
,
Mohammad Reza Fadaei Chafy
2
,
Amir Mohammad Moharrami
3
1 - Department of Physical Education and Sport Sciences, Ra.C., Islamic Azad University, Rasht, Iran
2 - Department of Physical Education and Sport Science, Ra.C., Islamic Azad University, Rasht, Iran
3 - Department of Physical Education and Sport Sciences, Ra.C., Islamic Azad University, Rasht, Iran
کلید واژه: Circadian rhythm, Exercise timing, Cortisol, Testosterone, Insulin, Resistance–aerobic training, Hormonal adaptation,
چکیده مقاله :
Introduction: Circadian rhythm is one of the factors interfering with hormonal responses to a training session. Therefore, the aim of the present study was to compare the effect of acute resistance-aerobic training in the morning and evening on the levels of cortisol, testosterone, and insulin hormones in male and female bodybuilders.
Material & Methods: Subjects included 15 male (age 27.70±3.99 years) and 15 female (age 25.90±4.10 years) bodybuilders voluntarily participated in this semi-experimental study. After obtaining informed consent, blood samples were collected before and after the training session in two separate times (8:00 a.m. and 5:00 p.m.), with a three-day recovery interval between sessions. resistance training was performed for 30 minutes, including upper and lower body resistance movements at 80% of one repetition maximum, and after a 3-minute rest, aerobic training was performed, including running on a treadmill for 20 minutes at an intensity of 75% of maximum heart rate. Blood samples were collected immediately before and after each session to assess serum cortisol, testosterone, and insulin concentrations using ELISA method. Data were analyzed using the Shapiro–Wilk test for normality, paired and independent t-tests, and repeated-measures ANOVA (p≤0.05).
Results: Between-group comparisons showed that, after a session of Resistance-Aerobic training, the decrease in both cortisol and insulin hormones was greater in women in the evening and in men in the morning (p<0.05). However, the increase in testosterone in both women and men was greater in the evening (p<0.05).
Conclusion: The results of the present study showed that circadian rhythm affects the responses of cortisol, testosterone, and insulin hormones to acute aerobic-resistance training in male and female bodybuilders; therefore, gender and training time in the morning and evening are intervening factors on different hormonal responses to a training session.
Introduction: Circadian rhythm is one of the factors interfering with hormonal responses to a training session. Therefore, the aim of the present study was to compare the effect of acute resistance-aerobic training in the morning and evening on the levels of cortisol, testosterone, and insulin hormones in male and female bodybuilders.
Material & Methods: Subjects included 15 male (age 27.70±3.99 years) and 15 female (age 25.90±4.10 years) bodybuilders voluntarily participated in this semi-experimental study. After obtaining informed consent, blood samples were collected before and after the training session in two separate times (8:00 a.m. and 5:00 p.m.), with a three-day recovery interval between sessions. resistance training was performed for 30 minutes, including upper and lower body resistance movements at 80% of one repetition maximum, and after a 3-minute rest, aerobic training was performed, including running on a treadmill for 20 minutes at an intensity of 75% of maximum heart rate. Blood samples were collected immediately before and after each session to assess serum cortisol, testosterone, and insulin concentrations using ELISA method. Data were analyzed using the Shapiro–Wilk test for normality, paired and independent t-tests, and repeated-measures ANOVA (p≤0.05).
Results: Between-group comparisons showed that, after a session of Resistance-Aerobic training, the decrease in both cortisol and insulin hormones was greater in women in the evening and in men in the morning (p<0.05). However, the increase in testosterone in both women and men was greater in the evening (p<0.05).
Conclusion: The results of the present study showed that circadian rhythm affects the responses of cortisol, testosterone, and insulin hormones to acute aerobic-resistance training in male and female bodybuilders; therefore, gender and training time in the morning and evening are intervening factors on different hormonal responses to a training session.
Chtourou H, Souissi N. The effect of training at a specific time of day: a review. Journal of strength and conditioning research. 2012;26(7):1984-2005. DOI: 10.1519/JSC.0b013e31825770a7
Ayala V, Martínez-Bebia M, Latorre JA, Gimenez-Blasi N, Jimenez-Casquet MJ, Conde-Pipo J, et al. Influence of circadian rhythms on sports performance. Chronobiology international. 2021;38(11):1522-36. DOI: 10.1080/07420528.2021.1931273
Takahashi JS. Transcriptional architecture of the mammalian circadian clock. Nature reviews Genetics. 2017;18(3):164-79. DOI: 10.1038/nrg.2016.150
Bird S, Tarpenning K. Effect of circadian rhythm on testosterone and cortisol response to a single bout of heavy resistance exercise in young men. Chronobiology international. 2003;20:1169-171. DOI: 10.1081/cbi-120025236
Kalsbeek A, la Fleur S, Fliers E. Circadian control of glucose metabolism. Molecular metabolism. 2014;3(4):372-83. DOI: 10.1016/j.molmet.2014.03.002
Nicolaides NC, Charmandari E, Kino T, Chrousos GP. Stress-Related and Circadian Secretion and Target Tissue Actions of Glucocorticoids: Impact on Health. Frontiers in endocrinology. 2017;8:70. DOI: 10.3389/fendo.2017.00070
Potter GD, Skene DJ, Arendt J, Cade JE, Grant PJ, Hardie LJ. Circadian Rhythm and Sleep Disruption: Causes, Metabolic Consequences, and Countermeasures. Endocrine reviews. 2016;37(6):584-608. DOI: 10.1210/er.2016-1083
Kim H-K, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, et al. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses. PLOS ONE. 2015;10(9):e0137567. DOI: 10.1371/journal.pone.0137567
Kanaley JA, Weltman JY, Pieper KS, Weltman A, Hartman ML. Cortisol and Growth Hormone Responses to Exercise at Different Times of Day1. The Journal of Clinical Endocrinology & Metabolism. 2001;86(6):2881-9. DOI: 10.1210/jcem.86.6.7566
Pledge D, Grosset J-F, Onambélé-Pearson GL. Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise? Cytokine. 2011;55(2):318-23. DOI: 10.1016/j.cyto.2011.05.005
Kim N, Ka S, Park J. Effects of exercise timing and intensity on physiological circadian rhythm and sleep quality: a systematic review. Physical activity and nutrition. 2023;27(3):52-63. DOI: 10.20463/pan.2023.0026
Crewther BT, Cook C, Cardinale M, Weatherby RP, Lowe T. Two emerging concepts for elite athletes: the short-term effects of testosterone and cortisol on the neuromuscular system and the dose-response training role of these endogenous hormones. Sports medicine (Auckland, NZ). 2011;41(2):103-23. DOI: 10.2165/11539170-000000000-00000
Hackney AC. Exercise as a stressor to the human neuroendocrine system. Medicina (Kaunas, Lithuania). 2006;42(10):788-97. PMID: 17090977
Vitale JA, Weydahl A. Chronotype, Physical Activity, and Sport Performance: A Systematic Review. Sports medicine (Auckland, NZ). 2017;47(9):1859-68. DOI: 10.1007/s40279-017-0741-z
De Luccia T. Use of the Testosterone/Cortisol Ratio Variable in Sports. The Open Sports Sciences Journal. 2016;9:104-13. DOI: 10.2174/1875399X01609010104
Kim HK, Radak Z, Takahashi M, Inami T, Shibata S. Chrono-exercise: Time-of-day-dependent physiological responses to exercise. Sports medicine and health science. 2023;5(1):50-8. DOI: 10.1016/j.smhs.2022.12.001
Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to resistance exercise and training. Sports medicine (Auckland, NZ). 2005;35(4):339-61. DOI: 10.2165/00007256-200535040-00004
Mennitti C, Farina G, Imperatore A, De Fonzo G, Gentile A, La Civita E, et al. How Does Physical Activity Modulate Hormone Responses? Biomolecules. 2024;14(11). DOI: 10.3390/biom14111466
Nicolaides NC, Kanaka-Gantenbein C, Pervanidou P. Developmental Neuroendocrinology of Early-Life Stress: Impact on Child Development and Behavior. 2024;22(3):461-74. DOI: 10.2174/1570159X21666230908094338
Hayes LD, Bickerstaff GF, Baker JS. Interactions of cortisol, testosterone, and resistance training: influence of circadian rhythms. Chronobiology international. 2010;27(4):675-705. DOI: 10.3109/07420521003778773
Triki R, Ben Abderrahman A, Salhi I, Rhibi F, Saeidi A, Almaqhawi A, et al. Effects of time-of-day resistance training on muscle strength, hormonal adaptations, and sleep quality during Ramadan fasting. Frontiers in nutrition. 2024;11:1439738. DOI: 10.3389/fnut.2024.1439738
Borghouts LB, Keizer HA. Exercise and insulin sensitivity: a review. International journal of sports medicine. 2000;21(1):1-12. DOI: 10.1055/s-2000-8847
Hong JY, Li YJ, Metcalfe RS, Chen YC. Effects of acute and chronic stair-climbing exercise on metabolic health: A systematic review. Journal of sports sciences. 2024;42(6):498-510. DOI: 10.1080/02640414.2024.2353974
