Identification of Streptococcus mutans bacteria by polymerase chain reaction in dental plaque samples through detection of the gene encoding glucosyltransferase enzyme
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
1 - گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد لاهیجان، لاهیجان، ایران
کلید واژه: Streptococcus mutans, dental plaque, saliva-DMFT, GTFB gene,
چکیده مقاله :
Dental caries is among the most common infectious diseases worldwide and remains a significant public health concern. Streptococcus mutans is widely recognized as a primary causative agent in the initiation and progression of dental caries. This study aimed to assess the prevalence of S. mutans in dental plaque and saliva samples from adults with varying caries activity and to explore the relationship between bacterial presence and caries severity.A total of 128 adult participants from diverse age groups underwent dental examinations following World Health Organization (WHO) criteria. Caries status was quantified using the DMFT index (decayed, missing, and filled teeth). Dental plaque and saliva samples were collected and analyzed for S. mutans using polymerase chain reaction (PCR) targeting the glucosyltransferase B (GTFB) gene. Data analysis was performed using SPSS software.The mean DMFT score among participants was 5.6, indicating moderate caries experience. S. mutans was detected in 16.17% of plaque and saliva samples. The presence of S. mutans correlated with higher caries activity, confirming its significant role in caries pathogenesis. Additionally, findings suggest that other mutans streptococci species may also contribute to dental caries development.This study highlights a notable prevalence of S. mutans in adults with varying caries levels, reinforcing its importance in caries progression. The results underscore the value of microbial profiling in caries risk assessment and suggest that targeting a broader range of mutans streptococci could improve preventive strategies.
Dental caries is among the most common infectious diseases worldwide and remains a significant public health concern. Streptococcus mutans is widely recognized as a primary causative agent in the initiation and progression of dental caries. This study aimed to assess the prevalence of S. mutans in dental plaque and saliva samples from adults with varying caries activity and to explore the relationship between bacterial presence and caries severity.A total of 128 adult participants from diverse age groups underwent dental examinations following World Health Organization (WHO) criteria. Caries status was quantified using the DMFT index (decayed, missing, and filled teeth). Dental plaque and saliva samples were collected and analyzed for S. mutans using polymerase chain reaction (PCR) targeting the glucosyltransferase B (GTFB) gene. Data analysis was performed using SPSS software.The mean DMFT score among participants was 5.6, indicating moderate caries experience. S. mutans was detected in 16.17% of plaque and saliva samples. The presence of S. mutans correlated with higher caries activity, confirming its significant role in caries pathogenesis. Additionally, findings suggest that other mutans streptococci species may also contribute to dental caries development.This study highlights a notable prevalence of S. mutans in adults with varying caries levels, reinforcing its importance in caries progression. The results underscore the value of microbial profiling in caries risk assessment and suggest that targeting a broader range of mutans streptococci could improve preventive strategies.
Vacca-Smith AM, Venkitaraman AR, Quivey RG Jr, Bowen WH: Interactions of streptococcal glucosyltransferases with alpha-amylase and starch on the surface of saliva-coated hydroxyapatite. Arch Oral Biol 1996a; 41: 291–298.
Van Hijum SA, Kralj S, Ozimek LK, Dijkhuizen L, van Geel-Schutten IG: Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria. Microbiol Mol Biol Rev 2006; 70: 157–176.
Van Hijum SA, Kralj S, Ozimek LK, Dijkhuizen L, van Geel-Schutten IG: Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria. Microbiol Mol Biol Rev 2006; 70: 157–176.
Van Houte J. Role of micro-organisms in caries etiology. J Dent Res. 1994; 73:672-81.
Venkitaraman AR, Vacca-Smith AM, Kopec LK, Bowen WH: Characterization of glucosyltransferase B, gtfC, and gtfD in solution and on the surface of hydroxyapatite. J Dent Res 1995; 74: 1695–1701.
Venkitaraman AR, Vacca-Smith AM, Kopec LK, Bowen WH: Characterization of glucosyltransferase B, gtfC, and gtfD in solution and on the surface of hydroxyapatite. J Dent Res 1995; 74: 1695–1701.
Xiao J, Koo H: Structural organization and dynamics of exopolysaccharide matrix and microcolonies formation by Streptococcus mutans in biofilms. J Appl Microbiol 2010; 108: 2103–2113.
Xiao J, Koo H: Structural organization and dynamics of exopolysaccharide matrix and microcolonies formation by Streptococcus mutans in biofilms. J Appl Microbiol 2010; 108: 2103–2113.
