بررسی تاثیرات گانودریک اسید و نانوذره مس بر تولید آفلاتوکسینB1 توسط آسپرژیلوس فلاووس
محورهای موضوعی : قارچ شناسی
محدثه لاری پور
1
,
الهه صبوری
2
,
تینا زرین پناه
3
,
حمیدرضا شیر محمدی
4
,
فرشته رحمتی
5
1 - گروه میکروبیولوژی، دانشگاه آزاد اسلامی تهران شمال، تهران، ایران
2 - دانشجوی کارشناسی ارشد، گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران
3 - گروه میکروبیولوژی، دانشگاه آزاد واحد اسلامی واحد بین‌المللی کیش، کیش، ایران.
4 - دانشجوی کارشناسی ارشد، گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران
5 - استادیار، گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران
کلید واژه: آفلاتوکسین B1, آسپرژیلوس فلاووس, گانودریک اسید, نانوذره مس, فعالیت ضدقارچی.,
چکیده مقاله :
سابقه و هدف: آفلاتوکسین B1 از مهمترین سموم قارچی که از طریق مصرف مواد غذایی به بدن انسان وارد شده واثرات سرطان زایی در انسان دارد. قارچ های خوراکی- دارویی کلاهدار حاوی مواد موثره با اثرات ضد میکروبی، ضد سرطانی می باشند. هدف این مطالعه بررسی اثرات بازدارندگی و ضد توکسین‌زایی گانودریک اسید مشتق از گانودرما لوسیدوم Ganoderma lucidum و نانوذره مس به‌صورت مستقل و هم‌افزا بر رشد آسپرژیلوس فلاووس Aspergillus flavus و تولید آفلاتوکسین B1 بود.
مواد و روش‌ها: در این پژوهش تجربی، گانودریک اسید و نانوذره مس بر اساس روش‌های استاندارد استخراج و آماده‌سازی شدند. فعالیت ضد قارچی با استفاده از آزمون‌های دیسک دیفیوژن، MIC، MFC و IC50 ارزیابی شد. برای سنجش سطح آفلاتوکسین B1، از کروماتوگرافی مایع با کارایی بالا (HPLC) استفاده گردید.
یافته‌ها: هر دو ترکیب به‌طور مؤثری رشد قارچ را مهار کردند. نانوذره مس در غلظت µg/ml 125 به‌طور کامل موجب مهار رشد شد (MIC)، در حالی‌که گانودریک اسید در غلظت µg/ml 250 به این اثر دست یافت. با این حال، نتایج HPLC نشان داد که گانودریک اسید به‌طور معنی‌داری آفلاتوکسین B1 را بیش از سایر تیمارها کاهش داد.
نتیجه‌گیری: گانودریک اسید می‌تواند به‌عنوان یک ترکیب زیستی، ایمن و مؤثر برای مهار آسپرژیلوس فلاووس و کاهش سطح آفلاتوکسین B1 در مواد غذایی و خوراک دام مطرح باشد. یافته‌ها نشان می‌دهد که افزودن نانوذره مس به این ترکیب، موجب بهبود معنادار عملکرد ضدتوکسین‌زایی نشده و تحقیقات آینده باید به بررسی دقیق‌تر مکانیسم‌های مولکولی این تعاملات بپردازد.
Background and Objective: Aflatoxin B1 is one of the most critical mycotoxins that enters the human body through the consumption of food and has carcinogenic effects in humans. Edible and medicinal macrofungi contain active compounds with antimicrobial and anticancer properties.The objective of this study was to investigate the inhibitory and antitoxigenic effects of ganoderic acid derived from Ganoderma lucidum and a copper nanoparticle (CuNP), both individually and synergistically, on the growth of Aspergillus flavus and the production of Aflatoxin B1.
Materials and Methods: I In this experimental research, ganoderic acid and the copper nanoparticle were extracted and prepared using standard methods. Antifungal activity was evaluated using the disk diffusion assay, Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and Inhibitory Concentration 50% (IC50) tests. High-Performance Liquid Chromatography (HPLC) was used to measure the level of Aflatoxin B1.
Results: Both compounds effectively inhibited fungal growth. The copper nanoparticle completely inhibited growth at a concentration of 125 µg/ml (MIC), while ganoderic acid achieved this effect at a concentration of 250 µg/ml. However, HPLC results indicated that ganoderic acid significantly reduced Aflatoxin B1 more than the other treatments
Conclusion: Ganoderic acid can be proposed as a safe, effective, and biological compound for inhibiting Aspergillus flavus and reducing Aflatoxin B1 levels in foodstuffs and animal feed. The findings suggest that the addition of the copper nanoparticle to this compound did not significantly improve the antitoxigenic performance, and future research should focus on a more precise investigation of the molecular mechanisms of these interactions.
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