The Effect of Using Different Zinc Sources on Performance, Blood Parameters, and Some Enzymes Related to the Immune System in Holstein Calves
محورهای موضوعی :F. Mirzaei Aghjehgheshlagh 1 , M. Pirshalkohi 2 , B. Navidshad 3 , H. Abdi Benmar 4 , J. Seifdavati 5 , A. Mahdavi 6
1 - Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
3 - Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
5 - Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran
6 - Department of Animal Science, Semnan University, Semnan, Iran
کلید واژه: blood parameters, Holstein calf, immune system, organic and inorganic forms of zinc,
چکیده مقاله :
This study aimed to evaluate the effects of different zinc sources on performance, blood parameters, and key immune system enzymes in calves. The experiment was conducted over a period of 70 days using 20 calves divided into four treatment groups. The treatments included: (1) a basal diet without zinc supplemen-tation, (2) a basal diet supplemented with 40 ppm zinc in the form of nano zinc oxide, (3) a basal diet sup-plemented with 40 ppm zinc in the form of zinc oxide, and (4) a basal diet supplemented with 40 ppm zinc in the form of zinc glycine. The results indicated that starter intake, total feed intake, daily weight gain, and final weight in calves fed diets containing different zinc forms showed significant improvement compared to the control group during the last month of the study (P<0.05). A significant difference was also observed between the control group and the groups receiving nano zinc oxide and zinc glycine during the second period of the experiment (P<0.05). Blood parameter analysis revealed that zinc supplementation had no significant effect. However, the zinc levels in the nano zinc oxide and zinc glycine groups were signifi-cantly higher compared to the control (P<0.05). Additionally, the inclusion of different zinc forms markedly influenced total antioxidant activity and superoxide dismutase concentration in calves (P<0.05). Overall, these findings suggest that dietary supplementation with various forms of zinc.
This study aimed to evaluate the effects of different zinc sources on performance, blood parameters, and key immune system enzymes in calves. The experiment was conducted over a period of 70 days using 20 calves divided into four treatment groups. The treatments included: (1) a basal diet without zinc supplemen-tation, (2) a basal diet supplemented with 40 ppm zinc in the form of nano zinc oxide, (3) a basal diet sup-plemented with 40 ppm zinc in the form of zinc oxide, and (4) a basal diet supplemented with 40 ppm zinc in the form of zinc glycine. The results indicated that starter intake, total feed intake, daily weight gain, and final weight in calves fed diets containing different zinc forms showed significant improvement compared to the control group during the last month of the study (P<0.05). A significant difference was also observed between the control group and the groups receiving nano zinc oxide and zinc glycine during the second period of the experiment (P<0.05). Blood parameter analysis revealed that zinc supplementation had no significant effect. However, the zinc levels in the nano zinc oxide and zinc glycine groups were signifi-cantly higher compared to the control (P<0.05). Additionally, the inclusion of different zinc forms markedly influenced total antioxidant activity and superoxide dismutase concentration in calves (P<0.05). Overall, these findings suggest that dietary supplementation with various forms of zinc.
Abdelnour S.A., Alagawany M., Hashem N.M., Farag M.R., Al-ghamdi E.S. Hassan F.U., Bilal R.M., Elnesr S.S., Dawood M.A.O. and Nagadi S.A. (2021). Nanominerals: Fabrication methods benefits and hazards, and their applications in rumi-nants with special reference to selenium and zinc nanoparti-cles. Animals. 11, 1916-1927.
Alimohamady R., Aliarabi H. and Bruckmaier R.M. (2019). Effect of different sources of supplemental zinc on performance, nu-trient digestibility, and antioxidant enzyme activities in lambs. Biol. Trace Elem. Res. 189, 75-86.
Altemimi A.B., Farag H.A.M., Salih T.H., Awlqadr F.H., Al-Manhel A.J.A., Vieira I.R.S. and Conte-Junior C.A. (2024). Application of nanoparticles in human nutrition: A review. Nutrients. 16, 636-652.
Anil T.S.V., Venkata Seshaiah C., Ashalatha P. and Sudhakar K. (2019). Effect of dietary supplementation of nano zinc oxide (nZnO) on growth performance in crossbred calves. Int. J. Curr. Microbiol. App. Sci. 8(12), 1852-1856.
Arabi H.A., Tabatabaei M.M., Fadaei-Far A., Torkashvand S., Bahari A.A., Zamani P., Alipour D. and Dezfulian A.H. (2011). The effect of adding organic zinc supplement with or without copper on performance, plasma mineral profile, and some enzyme activities in male Mehraban lambs. J. Anim. Sci. Res. 21(3), 111-122.
Arabi F., Imandar M., Negahdary M., Imanda M., Noughabi M.T., Akbari-dastjerdi H. and Fazilati M. (2012). Investigation of the anti-bacterial effect of zinc oxide nanoparticles upon the life of Listeria monocytogenes. Ann. Biol. Res. 3, 3679-3685.
Case C.L. and Carlson M.S. (2002). Effect of feeding organic and inorganic sources of additional zinc on growth performance and zinc balance in nursery pigs. J. Anim. Sci. 80, 1917-1924.
Elamin K.M., Dafalla N.A., Abdel Atti K.A. and Tameem Eldar A.A. (2013). Effects of zinc supplementation on growth per-formance and some blood parameters of goat kids in Sudan. Int. J. Pure Appl. Biol. Res. Sci. 1(1), 35-45.
Fadayifar A., Aliarabi H., Tabatabaei M.M., Zamani P., Bahari A.A., Malecki M. and Dezfoulian A.H. (2012). Improvement in lamb performance on barley-based diet supplemented with zinc. Livest. Sci. 144, 285-289.
Garg A.K. and Mudgal V.S. (2008). Effect of organic zinc sup-plementation on growth, nutrient utilization, and mineral pro-file in lambs. Anim. Feed Sci. Technol. 144, 82-96.
Glass G.A. and Gershon D. (1984). Decreased enzymic protection and increased sensitivity to oxidative damage in erythrocytes as a function of cell and donor aging. Bioch. J. 218, 513-537.
Hou P., Li B., Wang Y., Li D., Huang X., Sun W., Liang X. and Zhang E. (2023). The effect of dietary supplementation with zinc amino acids on immunity, antioxidant capacity, and gut microbiota composition in calves. Animals. 13, 1570-1578.
Hussan F., Krishna D., Preetam V.C., Reddy P.B. and Gurran S. (2022). Dietary supplementation of nano zinc oxide on per-formance, carcass, serum, and meat quality parameters of commercial broilers. Biol. Trace Elem. Res. 200, 348-353.
Kachhadia L., Katole S.B., Pandya P.R. and Patil G. (2023). Ef-fect of feeding different forms of zinc on growth performance of male crossbred calves. Indian J. Anim. Nutr. 39(4), 15-24.
Kegley E.B. and Spears J.W. (1994). Effect of zinc supplementa-tion on performance and zinc metabolism of lambs fed forage-based diets. J. Agric. Sci. 123, 287-292.
Kessler J., Morel I., Dufey P.A., Gutzwiller A., Stern A. and Geyer H. (2003). Effects of organic zinc sources on perform-ance, zinc status, and carcass, meat, and claw quality in fatten-ing bulls. Livest. Prod. Sci. 81, 161-171.
Kesler K.W. and Abuelo A. (2024). Zinc about it – zinc and calf immunity. Front. Immunol. 15, 1-11.
Khan S.A. (1978). Interaction of copper and zinc and its influence on the metabolism of major nutrients in growing calves. Ph D Thesis. Aligarh Muslim Univ., Aligarh, India.
Kincaid R.L., Chew B.P. and Cronrath J.D. (1997). Zinc oxide and amino acids as sources of dietary zinc for calves: Effects on uptake and immunity. J. Dairy Sci. 80, 1381-1388.
Konieczka P. (2004). Quality Assessment and Quality Control of Analytical Results. CDAims, Gdansk, Polish.
Lin W., Xu Y., Huang C., Ma Y., Shannon K.B. and Chen D. (2009). Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells. J. Nanopart. Res. 11, 25-39.
Liu J., Ma F., Degen A. and Sun P. (2023). The effects of zinc supplementation on growth, diarrhea, antioxidant capacity, and immune function in Holstein dairy calves. Animals. 13, 2493-2503.
Mandal G.P., Dass R.S., Isore D.P., Garg A.K. and Ram G.C. (2007). Effect of zinc supplementation from two sources on growth, nutrient utilization, and immune response in male crossbred cattle (Bos indicus×Bos taurus) bulls. Anim. Feed Sci. Technol. 138, 1-12.
Mattioli G.A., Rosa D.E., Turic E., Testa J.A., Lizarraga R.M. and Fazzio L.E. (2019). Effect of injectable copper and zinc sup-plementation on weight, hematological parameters, and im-mune response in pre-weaning beef calves. Biol. Trace Elem. Res. 189, 456-462.
Mishra A., Swain R.K., Mishra S.K., Panda N. and Sethy K. (2014). Growth performance and serum biochemical parame-ters as affected by nano zinc supplementation in layer chicks. Indian J. Anim. Nutr. 31, 384-388.
Nagalakshmi D., Dhanalakshmi K. and Himabindu D. (2009). Effect of dose and source of supplemental zinc on immune re-sponse and oxidative enzymes in lambs. Vet. Res. Commun. 33(7), 631-644.
Najafzadeh H., Ghoreishi M., Mohammadian B., Rahimi M., Afzalzadeh R., Kazemivarnamkhasti M. and Ganjealidarani H. (2013). Serum biochemical and histopathological changes in liver and kidney in lambs after zinc oxide nanoparticles ad-ministration. Vet. World. 6(8), 534-537.
Nazari M., Nikbaf-Shandiz M., Pashayee-Khamene F., Bagheri R., Goudarzi K., Hosseinnia N.V., Dolatshahi S., Omran H.S., Amirani N., Ashtary-larky D., Asbaghi O. and Ghanavati M. (2023). Zinc supplementation in individuals with prediabetes and type 2 diabetes: A GRADE-assessed systematic review and dose-response meta-analysis. Biol. Trace Elem. Res. 202, 2966-2990.
Nile S.H., Baskar V., Selvaraj D., Nile A., Xiao J. and Kai G. (2020). Nanotechnologies in food science: Applications, re-cent trends, and future perspectives. Nano-Micro Letters. 12, 45-55.
NRC. (2001). Nutrient Requirements of Dairy Cattle. 7th Ed. Na-tional Academy Press, Washington, DC., USA.
Ogbuewu I.P. and Mbajiorgu C.A. (2023). Potentials of Dietary zinc supplementation in improving growth performance, health status, and meat quality of broiler chickens. Biol. Trace Elem. Res. 201, 1418-1431
Olechnowicz J., Tinkov A., Skalny A. and Suliburska J. (2017). Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism. J. Physiol. Sci. 68(1), 19-31.
Pal D., Gowda T., Prasad N.K.S., Amarnath C.S., Bharadwaj R., SureshBabu U. and Sampath K.T. (2010). Effect of copper and zinc-methionine supplementation on bioavailability, mineral status and tissue concentrations of copper and zinc in ewes. J. Trace Elem. Med. Biol. 24, 89-94.
Parashar M., Shukla V.K. and Singh R. (2020). Metal oxides nanoparticles via sol–gel method: A review on synthesis, characterization and applications. J. Mater. Sci. Mater. Elec-tron. 31, 3729-3749.
Petrič D., Mikulová K., Bombárová A., Battanyi D., Cobanova K., Kopel P., Lukomska A., Pawlak P., Sidoruk P., Kotwica S., Cieslak A. and Varadyova Z. (2024). Efficacy of zinc nanoparticle supplementation on ruminal environment in lambs. BMC. Vet. Res. 20, 425-436.
Poudel R.R., Bhusal Y., Tharu B. and Kafle N.K. (2017). Role of zinc in insulin regulation and diabetes. J. Soc. Health Diabe-tes. 5(2), 123-130.
Puchala R., Sahlu T. and Davis J.J. (1999). Effects of zinc-methionine on performance of Angora goats. Small. Rumin. Res. 33, 1-8.
Rajaei-Sharifabadi H., Shamkhani E., Hafizi M., Mohammadi S., Shokri Z., Ahmadibonakdar Y. and Seradj A.R. (2024). Source-dependent effects of early-life zinc supplementation in milk on growth performance and starter intake of pre-weaned dairy calves. Front. Anim. Sci. 5, 1-9.
Rajendran D., Kumar G., Ramakrishna S. and Thomas K.S. (2013). Enhancing the milk production and immunity in Hol-stein Friesian crossbred cow by supplementing novel nano zinc oxide. Res. J. Biotechnol. 8, 11-17.
Ranasinghe P., Wathurapatha W.S., Ishara M.H., Jayawardana R., Galappttht P., Katulanda P. and Constantine G.R. (2015). Ef-fects of zinc supplementation on serum lipids: A systematic review and meta-analysis. Nutr. Metabol. 12, 26-35.
Rosi N.L. and Mirkin C.A. (2005). Nanostructures in biodiagnos-tics. Chem. Rev. 105, 1547-1562.
Sahoo A., Swain R.K., Mishra S.K. and Jena B. (2014). Serum biochemical indices of broiler birds fed on inorganic, organic and nano zinc supplemented diets. Int. J. Recent. Sci. Res. 5, 2078-2081.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sawai J., Kawada E., Kanou F., Igarashi H., Hashimoto A., Koku-gan T. and Shimizu M. (1996). Detection of active oxygen generated from ceramic powders having antibacterial activity. J. Chem. Eng. Jap. 29, 627-633.
Seyedalipour B., Ashrieh M. and Khanbabai R. (2014). The effect of 20 nm zinc oxide nanoparticles on enzymatic and tissue changes in the liver of pregnant NMRI mice. Yafteh J. Med. Sci. 16(3), 85-92.
Silva G.D., Rabello C.B.V., Wanderley J.S.D.S., Abreu K.L.D., Sousa L.F.A.D., Lima R.V.N., Costa F.S., Teixeira M.N., San-tos M.J.B.D. and Fireman A.K. (2024). Evaluating the effect of optimal zinc amino-acid complex supplementation in laying pullets on performance and zinc retention. PLoS One. 19(10), e0311813.
Singh K.K., Maity S.B. and Maity A. (2018). Effect of nano zinc oxide on zinc bioavailability and blood biochemical changes in pre-ruminant lambs. Indian J. Anim. Sci. 88(7), 805-807.
Smerchek D.T., Branine M.E., McGill J.L. and Hansen S.L. (2023). Effects of supplemental Zn concentration and trace mineral source on immune function and associated biomarkers of immune status in weaned beef calves received into a feed-lot. J. Anim. Sci. 101, 428-437.
Solaiman S.G. (2019). The effect of high levels of dietary zinc on growth performance, carcass characteristics, blood parameters, immune response and tissue minerals in growing Boer-cross goat kids. Small. Rumin. Res. 178, 1-8.
Song W., Zhang J., Guo J., Zhang J., Ding F., Li L. and Sun Z. (2010). Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicol. Lett. 199, 389-397.
Spears J.W. and Kegley E.B. (1991). Effect of zinc and manga-nese methionine on performance of beef cows and calves. J. Anim. Sci. 61(1), 59-65.
Suttle N. (2010). Mineral Nutrition of Livestock. Midlothian EH26 OPZ, United Kingdom.
Szuba-Trznadel A., Rząsa A., Hikawczuk T. and Fuchs B. (2021). Effect of zinc source and level on growth performance and zinc status of weaned piglets. Animals. 11, 2030-2041.
Thamizhan P., Jadhav S.E., Jadhav P.S., Chaudhary P., Choubey P.K., Singh V.K. and Dutta N. (2024). Influence of select die-tary trace minerals and their levels on growth performance, nutrient utilization and minerals balance in male goats. Biol. Trace. Elem. Res. 202, 3097-3106.
Wright C.L. and Spears J.W. (2004). Effect of zinc source and dietary level on zinc metabolism in Holstein calves. J. Dairy Sci. 87, 1085-1091.
Yang Z.P. and Sun L.P. (2006). Effects of nanometre ZnO on growth performance of early weaned piglets. J. Shanxi Agric. Sci. 3, 24-28.
Yusuf A.O., Adeyi T.K., Sowande O.S., Oni O.A. and Olowook-ere V.O. (2022). Nano zinc oxide supplementation improves growth performance and health of West African dwarf goats. Egyptian J. Anim. Prod. 59(2), 69-78.
Zaboli K. and Arabi H.A. (2013). The effect of different levels of zinc oxide nanoparticles and zinc oxide on some ruminal pa-rameters of male Marghoz goat kids by in vitro and in vivo methods. Anim. Prod. Res. 2(1), 1-14.
