بررسی نقش ژن FADDوc-FLIP در القای آپاپتوز توسط عصاره اتیل استات گیاه Artemisia biennis L. در رده سلولی SW480 سرطان کولورکتال
محورهای موضوعی : ژنتیک
مائده بازگیر اسب راهانی
1
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فرح فراهانی
2
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مریم خوش سخن مظفر
3
1 - دانشجو
2 - گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران.
3 - هیات علمی/ دانشگاه آزاد اسلامی واحد قم
کلید واژه: آپاپتوز, سرطان کولورکتال, Artemisia biennis L., MTT, بیان ژن, Real-Time PCR,
چکیده مقاله :
چکیده
هدف: با توجه به مقاومت برخی تومورها به درمانهای رایج، توجه به داروهای گیاهی ضدتوموری افزایش یافته است. این مطالعه به بررسی تأثیر عصاره اتیلاستات Artemisia biennis L. بر بیان ژنهای FADD و c-FLIP در سلولهای SW480 سرطان کولورکتال میپردازد.
روشها: سلولهای SW480 با عصاره اتیلاستات گیاه Artemisia biennis L. در غلظتهای ۲۵، ۵۰، ۱۰۰ و ۲۰۰ میکروگرم بر میلیلیتر و در بازههای زمانی ۲۴، ۴۸ و ۷۲ ساعت تیمار شدند. زندهمانی سلولها با آزمون MTT و محاسبه IC50، میزان آپاپتوز با رنگآمیزی تریپان بلو، و بیان ژنهای FADD و c-FLIP با روش Real-Time PCR ارزیابی گردید. تحلیل آماری دادهها با آزمون ANOVA انجام شد.
یافتهها: نتایج نشان داد که عصاره اتیلاستات در غلظتها و زمانهای بالاتر، موجب کاهش معنادار زندهمانی سلولها گردید؛ بهطوریکه بیشترین اثر مهاری در غلظت ۲۰۰ میکروگرم بر میلیلیتر و پس از ۷۲ ساعت مشاهده شد. بررسی بیان ژنها نیز حاکی از افزایش معنادار سطح بیان ژن FADD بود، در حالیکه تغییر قابل توجهی در بیان ژن c-FLIP مشاهده نگردید.
نتیجهگیری: عصاره اتیلاستات A. biennis با القای آپوپتوز از طریق افزایش بیان FADD، پتانسیل ضدسرطانی در درمان سرطان کولورکتال دارد. مطالعات بیشتر برای شناسایی مکانیزمهای مولکولی و ترکیبات فعال ضروری است.
Abstract
Objective: Due to the resistance of some tumors to common treatments, attention to herbal antitumor drugs has increased. This study investigates the effect of ethyl acetate extract of Artemisia biennis L. on the expression of FADD and c-FLIP genes in SW480 colorectal cancer cells.
Methods: SW480 cells were treated with ethyl acetate extract of Artemisia biennis L. at concentrations of 25, 50, 100, and 200 μg/mL for 24, 48, and 72 hours. Cell viability was assessed by MTT assay and IC50 calculation, apoptosis rate by trypan blue staining, and expression of FADD and c-FLIP genes by Real-Time PCR. Statistical analysis of data was performed by ANOVA test.
Results: The results showed that ethyl acetate extract at higher concentrations and times significantly reduced cell viability; The highest inhibitory effect was observed at a concentration of 200 μg/ml and after 72 hours. Gene expression analysis also indicated a significant increase in FADD gene expression, while no significant change was observed in c-FLIP gene expression.
Conclusion: A. biennis ethyl acetate extract has anticancer potential in the treatment of colorectal cancer by inducing apoptosis through increasing FADD expression. Further studies are necessary to identify the molecular mechanisms and active compounds.
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