Physiological and morphophysiological responses of guar (Cyamopsis tetragonoloba L.) to cobalt and jasmonic acid
محورهای موضوعی : Plant PhysiologyMohamed Baqer Hussine Almosawi 1 , Heidar Meftahizade 2
1 - College of Education for Pure Science, Al-Muthanna University, Samawah 88001, Iraq.
2 - Ardakan University
کلید واژه: antioxidant, heavy metal, photosynthesis, stress, toxicity,
چکیده مقاله :
Cobalt (Co) toxicity presents a substantial challenge to agricultural output, especially in economically significant crops like guar (Cyamopsis tetragonoloba L.). This research explored the capacity of jasmonic acid (JA) to lessen the detrimental impacts of cobalt-induced stress on the physiological and morphophysiological attributes of guar plants. Guar seedlings were subjected to varying levels of Co (0, 50, 100, and 200 µM) and treated with JA (0, 5, 10, and 15 µM). The results indicated that elevated Co concentrations significantly diminished plant growth, biomass accumulation, gum and carbohydrate levels, and water content while simultaneously increasing proline, catalase, peroxidase, phenol, and superoxide dismutase activities, suggesting the presence of oxidative stress. In contrast, applications of JA, particularly at lower concentrations (5 and 10 µM), successfully ameliorated these adverse effects by enhancing water status, reducing cobalt absorption, strengthening antioxidant defense mechanisms, and improving photosynthetic performance. Notably, JA treatment sustained or improved biomass, carbohydrate content, water content, and increased viscosity under Co stress conditions. These results highlight the potential of JA as a bio stimulant to improve guar plant tolerance against Co toxicity, offering a viable approach for sustainable agricultural practices in contaminated settings.
Cobalt (Co) toxicity presents a substantial challenge to agricultural output, especially in economically significant crops like guar (Cyamopsis tetragonoloba L.). This research explored the capacity of jasmonic acid (JA) to lessen the detrimental impacts of cobalt-induced stress on the physiological and morphophysiological attributes of guar plants. Guar seedlings were subjected to varying levels of Co (0, 50, 100, and 200 µM) and treated with JA (0, 5, 10, and 15 µM). The results indicated that elevated Co concentrations significantly diminished plant growth, biomass accumulation, gum and carbohydrate levels, and water content while simultaneously increasing proline, catalase, peroxidase, phenol, and superoxide dismutase activities, suggesting the presence of oxidative stress. In contrast, applications of JA, particularly at lower concentrations (5 and 10 µM), successfully ameliorated these adverse effects by enhancing water status, reducing cobalt absorption, strengthening antioxidant defense mechanisms, and improving photosynthetic performance. Notably, JA treatment sustained or improved biomass, carbohydrate content, water content, and increased viscosity under Co stress conditions. These results highlight the potential of JA as a bio stimulant to improve guar plant tolerance against Co toxicity, offering a viable approach for sustainable agricultural practices in contaminated settings.
Abdelgawad, H., C. Crecchio, M. Nhs, M. A. Abdel-Maksoud, A. Malik, M. S. Sheteiwy, Y. A. Hamoud, S. Sulieman, H. Shaghaleh and M. Alyafei. 2024. Mitigating gadolinium toxicity in guar (Cyamopsis tetragonoloba L.) through the symbiotic associations with arbuscular mycorrhizal fungi: physiological and biochemical insights. BMC Plant Biology, 24, (1) 877.
Ahmad, P., M. N. Alyemeni, L. Wijaya, P. Alam, M. A. Ahanger and S. A. Alamri. 2017. Jasmonic acid alleviates negative impacts of cadmium stress by modifying osmolytes and antioxidants in faba bean (Vicia faba L.).
Archives of Agronomy and Soil Science, 63, (13) 1889-1899.
Ahmad, P., V. Raja, M. Ashraf, L. Wijaya, A. Bajguz and M. N. Alyemeni. 2021. Jasmonic acid (JA) and gibberellic acid (GA3) mitigated Cd-toxicity in chickpea plants through restricted cd uptake and oxidative stress management. Scientific Reports, 11, (1) 19768.
Arnon, D. I. 1967. Photosynthetic activity of isolated chloroplasts. Physiological Reviews, 47, (3) 317-358.
Ashraf, F. and E. H. Siddiqi. 2024. Mitigation of drought-induced stress in sunflower (Helianthus annuus L.) via foliar application of Jasmonic acid through the augmentation of growth, physiological, and biochemical attributes. BMC Plant Biology, 24, (1) 592.
Banerjee, A. and A. Roychoudhury. 2021. Beneficial aspects of cobalt uptake in plants exposed to abiotic stresses. In Frontiers in Plant-Soil Interaction:523-529: Elsevier. Number of 523-529 pp.
Denton-Thompson, S. M. and E. J. Sayer. 2022. Micronutrients in food production: what can we learn from natural ecosystems? Soil Systems, 6, (1) 8.
Đurić, M., A. Subotić, L. Prokić, M. Trifunović-Momčilov and S. Milošević. 2023. Alterations in physiological, biochemical, and molecular responses of Impatiens walleriana to drought by methyl jasmonate foliar application. Genes, 14, (5) 1072.
Elshamly, A. M. 2023. Cobalt combined with potassium humate as beneficial applications in alleviating water stress impacts on groundnut during sensitive growth stages. Journal of Soil Science and Plant Nutrition, 23, (3) 4505-4520.
Farasati Far, B. 2024. Antioxidant Activity of Metal-Organic Frameworks. In Logic for Metal− Organic Framework Selection: MOFs for Biomedical Applications:107-146: ACS Publications. Number of 107-146 pp.
Gad, N., A. Sekara and M. T. Abdelhamid. 2019. The potential role of cobalt and/or organic fertilizers in improving the growth, yield, and nutritional composition of Moringa oleifera. Agronomy, 9, (12) 862.
Genchi, G., G. Lauria, A. Catalano, A. Carocci and M. S. Sinicropi. 2023. Prevalence of cobalt in the environment and its role in biological processes. Biology, 12, (10) 1335.
Hadwan, M. H. 2018. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC biochemistry, 19, 1-8.
Hanaka, A., W. Maksymiec and W. Bednarek. 2015. The effect of methyl jasmonate on selected physiological parameters of copper-treated Phaseolus coccineus plants. Plant Growth Regulation, 77, 167-177.
Hatamman, F. D. and A. A. Abdullah.2021. Effect of soil mulching and cobalt on growth and yield of (Phaseolus vulgaris L.) under protected conditions. Proc. IOP Conference Series: Earth and Environmental Science, 910:012097: IOP Publishing.
Hosseinifard, M., S. Stefaniak, M. Ghorbani Javid, E. Soltani, Ł. Wojtyla and M. Garnczarska. 2022. Contribution of exogenous proline to abiotic stresses tolerance in plants: A review. International Journal of Molecular Sciences, 23, (9) 5186.
Hu, X., X. Wei, J. Ling and J. Chen. 2021. Cobalt: an essential micronutrient for plant growth? Frontiers in plant science, 12, 768523.
Hu, Y.-Y., Y. Song, D.-S. Yu, W.-Z. Qian, M. He, L. Yi and S. Gao. 2023. Oxidative stress induced by cobalt in Jatropha curcas seedlings: response of growth and chemical changes. Journal of Elementology, 28, (4)
Inayat, H., H. Mehmood, S. Danish, S. A. Alharbi, M. J. Ansari and R. Datta. 2024. Impact of cobalt and proline foliar application for alleviation of salinity stress in radish. BMC Plant Biology, 24, (1) 287.
Ismail, L. M., M. I. Soliman, M. H. Abd El-Aziz and H. M. Abdel-Aziz. 2022. Impact of silica ions and nano silica on growth and productivity of pea plants under salinity stress. Plants, 11, (4) 494.
Jobe, M. C., D. M. Mthiyane, P. V. Dludla, S. E. Mazibuko-Mbeje, D. C. Onwudiwe and M. Mwanza. 2023. Pathological role of oxidative stress in aflatoxin-induced toxicity in different experimental models and protective effect of phytochemicals: a review. Molecules, 28, (14) 5369.
Khurizadeh, S., R. Naderi, H. Meftahizadeh, S. Hazrati and S. Nicola. 2024. Foliar Application of Methyl Jasmonate and Chitosan Improve Growth, Yield, and Quality of Guar (Cyamopsis tetragonoloba L.) Under Water-Deficit Stress. Plants, 13, (21) 3099.
Kosiorek, M. and M. Wyszkowski. 2021. Macroelement content in plants after amendment application to cobalt-contaminated soil. Journal of Soils and Sediments, 21, 1769-1784.
Kubi, H. a. A., M. A. Khan, A. Adhikari, M. Imran, S.-M. Kang, M. Hamayun and I.-J. Lee. 2021. Silicon and plant growth-promoting rhizobacteria Pseudomonas psychrotolerans CS51 mitigates salt stress in Zea mays L. Agriculture, 11, (3) 272.
Kumari, S., M. K. Manglam, L. Kumar, P. Seal, J. P. Borah, M. Kumar Zope and M. Kar. 2022. Magnetic properties and hyperthermia action of cobalt zinc ferrite fibers. Journal of Sol-Gel Science and Technology, 101, (3) 546-561.
Linh, T. M., N. C. Mai, P. T. Hoe, L. Q. Lien, N. K. Ban, L. T. T. Hien, N. H. Chau and N. T. Van. 2020. Metal‐based nanoparticles enhance drought tolerance in soybean. Journal of Nanomaterials, 2020, (1) 4056563.
Liu, H. and M. P. Timko. 2021. Jasmonic acid signaling and molecular crosstalk with other phytohormones. International Journal of Molecular Sciences, 22, (6) 2914.
Mahmood, F., S. S. Zehra, M. Hasan, A. Zafar, T. Tariq, M. Abdullah, M. A. Nazir, M. Jamil, S. G. Hassan and X. Huang. 2023. Bioinspired cobalt oxide nanoball synthesis, characterization, and their potential as metal stress absorbants. ACS omega, 8, (6) 5836-5849.
Meftahizadeh, H., G. S. Baath, R. K. Saini, M. Falakian and M. Hatami. 2023. Melatonin-mediated alleviation of soil salinity stress by modulation of redox reactions and phytochemical status in guar (Cyamopsis tetragonoloba L.). Journal of plant growth regulation, 42, (8) 4851-4869.
Meftahizadeh, H., M. Ghorbanpour and M. H. Asareh. 2019. Comparison of morphological and phytochemical characteristics in guar (Cyamopsis tetragonoloba L.) landraces and cultivars under different sowing dates in an arid environment. Industrial Crops and Products, 140, 111606.
Meftahizadeh, H. and M. Hatami. 2022. Changes in agronomic characteristics and galactomannan content in twenty cluster bean genotypes of different origins affected by sowing dates. Acta Ecologica Sinica, 42, (1) 24-32.
Miliauskas, G., P. Venskutonis and T. Van Beek. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry, 85, (2) 231-237.
Morgun, V., S. Kots, T. Mamenko, L. Rybachenko and P. Pukhtaievych. 2021. Regulation of superoxide dismutase activity in soybean plants by inoculating seeds with rhizobia containing nanoparticles of metal carboxylates under conditions of different water supply. Biosystems Diversity, 29, (1) 33-38.
Pandey, A., M. Agrawal and S. B. Agrawal. 2023. Ultraviolet-B and heavy metal-induced regulation of secondary metabolites in medicinal plants: a review. Metabolites, 13, (3) 341.
Raza, A., S. Charagh, S. Najafi-Kakavand and M. H. Siddiqui. 2021. The crucial role of jasmonates in enhancing heavy metals tolerance in plants. Jasmonates and salicylates signaling in plants, 159-183.
Rehman, M., M. S. Saeed, X. Fan, A. Salam, R. Munir, M. U. Yasin, A. R. Khan, S. Muhammad, B. Ali and I. Ali. 2023. The multifaceted role of jasmonic acid in plant stress mitigation: An overview. Plants, 12, (23) 3982.
Salam, A., M. S. Afridi, A. R. Khan, W. Azhar, Y. Shuaiqi, Z. Ulhassan, J. Qi, N. Xuo, Y. Chunyan and N. Chen. 2023. Cobalt induced toxicity and tolerance in plants: insights from omics approaches. Heavy Metal Toxicity and Tolerance in Plants: A Biological, Omics, and Genetic Engineering Approach, 207-229.
Sarraf, M., E. Janeeshma, N. Arif, M. Q. U. Farooqi, V. Kumar, N. A. Ansari, M. I. Ghani, M. A. Ahanger and M. Hasanuzzaman. 2023. Understanding the role of beneficial elements in developing plant stress resilience: Signalling and crosstalk with phytohormones and microbes. Plant Stress, 10, 100224.
Selim, S., N. Akhtar, N. Hagagy, A. Alanazi, M. Warrad, E. El Azab, M. Y. M. Elamir, M. M. Al-Sanea, S. K. A. Jaouni and M. Abdel-Mawgoud. 2022. Selection of newly identified growth-promoting Archaea haloferax species with a potential action on cobalt resistance in maize plants. Frontiers in Plant Science, 13, 872654.
Sheteiwy, M. S., H. Shao, W. Qi, P. Daly, A. Sharma, H. Shaghaleh, Y. A. Hamoud, M. A. El‐Esawi, R. Pan and Q. Wan. 2021. Seed priming and foliar application with jasmonic acid enhance salinity stress tolerance of soybean (Glycine max L.) seedlings. Journal of the Science of Food and Agriculture, 101, (5) 2027-2041.
Silva, S., M. C. Dias and A. M. Silva. 2022. Titanium and zinc based nanomaterials in agriculture: A promising approach to deal with (a) biotic stresses? Toxics, 10, (4) 172.
Swain, R., S. Sahoo, M. Behera and G. R. Rout. 2023. Instigating prevalent abiotic stress resilience in crop by exogenous application of phytohormones and nutrient. Frontiers in Plant Science, 14, 1104874.
Tamindžić, G., S. Azizbekian, D. Miljaković, M. Ignjatov, Z. Nikolić, D. Budakov, S. Vasiljević and M. Grahovac. 2024. Assessment of Various Nanoprimings for Boosting Pea Germination and Early Growth in Both Optimal and Drought-Stressed Environments. Plants, 13, (11) 1547.
Tomić, D., V. Stevović, D. Đurović, R. Stanisavljević, M. Madić, M. Petrović, Đ. Lazarević and J. Knežević. 2020. Seed testing of foliar-fertilised red clover crops after various periods of storage. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48, (1) 284-293.
Upadhyay, R., R. Saini, P. Shukla and K. Tiwari. 2025. Role of secondary metabolites in plant defense mechanisms: A molecular and biotechnological insights. Phytochemistry Reviews, 24, (1) 953-983.
Vega, A., N. Delgado and M. Handford. 2022. Increasing heavy metal tolerance by the exogenous application of organic acids. International Journal of Molecular Sciences, 23, (10) 5438.
Wang, Y., S. Mostafa, W. Zeng and B. Jin. 2021. Function and mechanism of jasmonic acid in plant responses to abiotic and biotic stresses. International Journal of Molecular Sciences, 22, (16) 8568.
Wasternack, C. and M. Strnad. 2019. Jasmonates are signals in the biosynthesis of secondary metabolites—Pathways, transcription factors and applied aspects—A brief review. New biotechnology, 48, 1-11.
Yavaş, İ., S. Ali, Z. Abbas and S. Hussain. 2022. Heavy metal toxicity in plants: an overview on tolerance mechanisms and management strategies.
Yu, X., W. Zhang, Y. Zhang, X. Zhang, D. Lang and X. Zhang. 2018. The roles of methyl jasmonate to stress in plants. Functional Plant Biology, 46, (3) 197-212.
Zahid, A., K. Ul Din, M. Ahmad, U. Hayat, U. Zulfiqar, S. M. H. Askri, M. Z. Anjum, M. F. Maqsood, N. Aijaz and T. Chaudhary. 2024. Exogenous application of sulfur-rich thiourea (STU) to alleviate the adverse effects of cobalt stress in wheat. BMC Plant Biology, 24, (1) 126.
Zhu, L., J. Guo, Y. Sun, S. Wang and C. Zhou. 2021. Acetic acid-producing endophyte Lysinibacillus fusiformis orchestrates jasmonic acid signaling and contributes to repression of cadmium uptake in tomato plants. Frontiers in Plant Science, 12, 670216.
