Effects of Inoculation and Fermentation Time on in vitro Digestibility, Microbial Population and Rumen Fermentation Characteristics of Industrial Potato Waste
محورهای موضوعی :
S.A. Muhammad
1
,
I.B. Suyub
2
,
F. Nobilly
3
,
H. Yaakub
4
1 - Department of Animal Science, Universiti Putra Malaysia, Malaysia
2 - Department of Animal Science, Universiti Putra Malaysia, Malaysia
3 - Department of Animal Science, Universiti Putra Malaysia, Malaysia
4 - Department of Animal Science, Universiti Putra Malaysia, Malaysia
کلید واژه: biohydrogenation, fermentation, inoculation, rumen metabolites,
چکیده مقاله :
Potato processing generates waste that is estimated to be around 12-20% of the original potato weight. The waste can further be processed or incorporated into animal feed formulations. However, there is limited information on potentials of industrial potato waste (IPW) as ruminant feedstuff. The study aimed to deter-mine the effect of inoculation and fermentation time on in vitro organic matter digestibility (IVOMD), me-tabolizable energy (ME), rumen microbial population and ruminal fermentation characteristics. The ex-periment involved inoculation of IPW with zero inoculum (control), Lactiplantibacillus plantarum (MW296876), Saccharomyces cerevisiae (MW296931) and Aspergillus oryzae (MW297015). The experi-mental design was completely randomized design (CRD) with factorial arrangement (4 treatments×5 repli-cations×4 fermentation time). After inoculation and fermentation, the substrates were subjected to anaerobic incubation, and gas volumes were recorded at 3, 6, 12, 24, 48 and 72 h. The results revealed that there was no significant (P>0.05) interaction between treatment and fermentation time on gas production and IVOMD. The rumen microbial population revealed that total bacteria, total methanogens, Ruminococcus flavafaciens, Ruminococcus albus and Fibrobacter succinogens had no significant (P>0.05) interaction between the effect of treatment and fermentation time. However, total protozoa, total fungi and Butyrivibrio fibrisolvens exhibited a significant (P<0.05) interaction. Although the methane content (7.11±1.49-8.07±0.32 mM) of A. oryzae did not change across the fermentation time, the values recorded were lowest (P<0.05) compared to 7.77 - 13.03 mM recorded for the other treatments. A. oryzae recorded highest (P<0.05) concentration (1299.40-2085.29 µg/100 mL) of C18:0 (stearic acid) across all the fermentation time. It was concluded that microbial inoculation of IPW affects net gas production, it improves biohydro-genation process and reduces methane production. Among the three inocula used, A. oryzae is recom-mended because it recorded highest content of stearic acid via biohydrogenation process, and reduced methane gas production.
Potato processing generates waste that is estimated to be around 12-20% of the original potato weight. The waste can further be processed or incorporated into animal feed formulations. However, there is limited information on potentials of industrial potato waste (IPW) as ruminant feedstuff. The study aimed to deter-mine the effect of inoculation and fermentation time on in vitro organic matter digestibility (IVOMD), me-tabolizable energy (ME), rumen microbial population and ruminal fermentation characteristics. The ex-periment involved inoculation of IPW with zero inoculum (control), Lactiplantibacillus plantarum (MW296876), Saccharomyces cerevisiae (MW296931) and Aspergillus oryzae (MW297015). The experi-mental design was completely randomized design (CRD) with factorial arrangement (4 treatments×5 repli-cations×4 fermentation time). After inoculation and fermentation, the substrates were subjected to anaerobic incubation, and gas volumes were recorded at 3, 6, 12, 24, 48 and 72 h. The results revealed that there was no significant (P>0.05) interaction between treatment and fermentation time on gas production and IVOMD. The rumen microbial population revealed that total bacteria, total methanogens, Ruminococcus flavafaciens, Ruminococcus albus and Fibrobacter succinogens had no significant (P>0.05) interaction between the effect of treatment and fermentation time. However, total protozoa, total fungi and Butyrivibrio fibrisolvens exhibited a significant (P<0.05) interaction. Although the methane content (7.11±1.49-8.07±0.32 mM) of A. oryzae did not change across the fermentation time, the values recorded were lowest (P<0.05) compared to 7.77 - 13.03 mM recorded for the other treatments. A. oryzae recorded highest (P<0.05) concentration (1299.40-2085.29 µg/100 mL) of C18:0 (stearic acid) across all the fermentation time. It was concluded that microbial inoculation of IPW affects net gas production, it improves biohydro-genation process and reduces methane production. Among the three inocula used, A. oryzae is recom-mended because it recorded highest content of stearic acid via biohydrogenation process, and reduced methane gas production.
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