Analysis of Disaccharides Interaction with Human Growth Hormone by Spectroscopy and Molecular Docking
محورهای موضوعی : شیمی کاربردیسولماز کامرانی 1 , پریچهره یغمایی 2 , مریم نیکخواه 3 , Saman Hosseinlhani 4
1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه نانوبیوتکنولوژی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
4 - گروه بیوشیمی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
کلید واژه: Molecular Docking, Somatotropin, Stability, Sucrose, Trehalose,
چکیده مقاله :
Sucrose and trehalose are disaccharides commonly used as stabilizer excipients in the formulation of therapeutic proteins. One of the essential therapeutic proteins that scientists focus on is the recombinant human growth hormone (rhGH). In the current study, we used UV spectroscopy and molecular docking simulation to investigate the effect of sucrose and trehalose on the stability of rhGH. The results showed that sucrose and trehalose could increase the thermal stability of rhGH. However, the aggregation of growth hormone in the presence of sucrose is less than in trehalose. Molecular docking studies showed that these differences could result from the interactions between growth hormone and the disaccharide. So, hGH has more regions that interact with sucrose than trehalose.
Sucrose and trehalose are disaccharides commonly used as stabilizer excipients in the formulation of therapeutic proteins. One of the essential therapeutic proteins that scientists focus on is the recombinant human growth hormone (rhGH). In the current study, we used UV spectroscopy and molecular docking simulation to investigate the effect of sucrose and trehalose on the stability of rhGH. The results showed that sucrose and trehalose could increase the thermal stability of rhGH. However, the aggregation of growth hormone in the presence of sucrose is less than in trehalose. Molecular docking studies showed that these differences could result from the interactions between growth hormone and the disaccharide. So, hGH has more regions that interact with sucrose than trehalose.
