Preconcentration and Determination of Biperiden by using Solvent Bar Micro Extraction Combined with HPLC in Biological Fluids and Optimization by Chemometrics Design
محورهای موضوعی : شیمی داروئیملیکا محمدی 1 , persia behbahani 2 , سید علی سبحانیان 3
1 - دانشکده علوم دارویی، گروه شیمی دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - Department of Chemistry, Sciences Faculty, Arak Branch, Islamic Azad University, Arak, Iran
3 - دانشکده علوم دارویی، گروه شیمی دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: Biperiden, HPLC, Solvent Bar Microextraction, Preconcentration,
چکیده مقاله :
Bipridein (1-Bicyclo[2.2.1]hept-5-en-2-yl-1-phenyl-3-piperidin-1-yl-propan-1-ol, I) is an anti-muscarinic and competitive acetylcholine antagonist agent in central receptors. Its main indication is treatment of drug-induced Parkinsonism and treatment of Parkinson's disease symptoms, which improves the motor symptoms of the disease. Because the blood concentration of this compound is very low, precise methods should be used to measure it. In this study, a combination of solvent bar micro extraction (SBME) and high performance liquid chromatography (HPLC) have been used to concentrate and measuring small amounts of this drug in biological fluids by chemometric methods. At the first, the drug was extracted from aqueous solution containing bipyridine at pH = 9.78 by organic solvent embedded in the hollow fiber pores. Then, in order to concentrate, the drug was transferred to the aqueous phase at pH = 2.98 which was contained in the hollow fiber by a back extraction procedure.Results showed that after extraction under the optimum conditions, pre-concentration factor was 94, limit of detection was 0.015 mg/L and relative standard deviation was 4.1-4.7%. It could be concluded that preconcentration and determination of Biperiden by using solvent bar micro extraction combined with HPLC in biological fluids has been effective.
Bipridein (1-Bicyclo[2.2.1]hept-5-en-2-yl-1-phenyl-3-piperidin-1-yl-propan-1-ol, I) is an anti-muscarinic and competitive acetylcholine antagonist agent in central receptors. Its main indication is treatment of drug-induced Parkinsonism and treatment of Parkinson's disease symptoms, which improves the motor symptoms of the disease. Because the blood concentration of this compound is very low, precise methods should be used to measure it. In this study, a combination of solvent bar micro extraction (SBME) and high performance liquid chromatography (HPLC) have been used to concentrate and measuring small amounts of this drug in biological fluids by chemometric methods. At the first, the drug was extracted from aqueous solution containing bipyridine at pH = 9.78 by organic solvent embedded in the hollow fiber pores. Then, in order to concentrate, the drug was transferred to the aqueous phase at pH = 2.98 which was contained in the hollow fiber by a back extraction procedure.Results showed that after extraction under the optimum conditions, pre-concentration factor was 94, limit of detection was 0.015 mg/L and relative standard deviation was 4.1-4.7%. It could be concluded that preconcentration and determination of Biperiden by using solvent bar micro extraction combined with HPLC in biological fluids has been effective.
