تغییرات VEGF و HIF- α1 در بافت قلب موش های صحرایی ماده سالمند به دنبال هشت هفته تمرین تناوبی شدید و مصرف جنستئین
محورهای موضوعی : نقش بیان ژن در سلامتیباقر سهیلی 1 , سید علی حسینی 2 , محمود همت فر 3
1 - دانشجوی دکتری گروه فیزیولوژی ورزش، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران،
2 - گروه مدیریت ورزش، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران.
3 - گروه حسابداری، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
کلید واژه: تمرین, جنستئین, آنژیوژنز, قلب, سالمندی,
چکیده مقاله :
مقدمه: اگرچه نقش تمرین و آنتیاکسیدانها بر بهبود عملکرد قلب گزارش شده است. اما اثر همزمان آنها هنوز به خوبی شناخته نشده است. بنابراین مطالعه حاضر با هدف تغییرات VEGF و HIF- α1 در بافت قلب موشهای صحرایی ماده سالمند به دنبال هشت هفته تمرین تناوبی شدید و مصرف جنستئین انجام شد.
روشها: در این مطالعه تجربی 30 سر موش صحرایی ماده 18-24 ماهه نژاد اسپراگداولی انتخاب و به گروه های (1) HIIT، (2) جنستئین، (3) HIIT+مصرف جنستئین، (4) کنترل سالم و (5) شم تقسیم شدند. در مدت 8 هفته گروههای 2 و 3 روزانه mg/kg 60 جنستئین حل شده در DMSO و همچنین گروه 5 روزانه mg/kg 60 DMSO به صورت داخل صفاقی دریافت کردند. گروههای 1 و 3 به ميزان 3 جلسه در هفته روی نوارگردان دویدند. جهت تجزیه و تحلیل یافتهها از آزمونهای آماری آنالیز واریانس یکطرفه همراه با آزمون تعقیبی توکی استفاده شد (05/0>P).
نتایج: بیان VEGF و HIF-1α قلبی در گروه HIIT نسبت به گروه کنترل افزایش یافت (05/0>P). همچنین در گروه HIIT+جنستئین مقادیر VEGF بیشتر از گروه کنترل بود (05/0>P). همچنین در گروه HIIT مقادیر VEGF و HIF-1α قلبی بیشتر از گروه ترکیب تمرین + جنستئین بود (05/0>P).
نتیجهگیری: به نظر میرسد تمرین تناوبی شدید دارای اثرات آنژیوژنیک است اما جنستئین با دوز مطالعه حاضر منجر به تعدیل آنژیوژنز ناشی از ورزش میشود.
Introduction: Although the role of exercise and antioxidants in improving cardiac function has been reported. But their simultaneous effect is still not well known. Therefore, the present study was conducted with the aim of VEGF and HIF-α1 changes in the heart tissue of elderly female rats after eight weeks of intense intermittent exercise and genistein consumption.
Methods: In this experimental study, 30 female Sprague-Dawley rats aged 18-24 months divided into five groups including (1) HIIT, (2)genistein, (3)HIIT with genistein, (4) healthy control and (5) sham. During 8 weeks, groups 2 and 3 received 60 mg/kg genistein dissolved in DMSO daily, and group 5 received 60 mg/kg DMSO intraperitoneally. Groups 1 and 3 ran on the treadmill 3 times a week. To analyze the findings, statistical tests of one-way analysis of variance with Tukey's post hoc test were used(P<0.05).
Results: The expression of cardiac VEGF and HIF-1α increased in the intense interval training group compared to the control group. Also, in the exercise + genistein group, VEGF values were significantly higher than the control group. In addition, in the exercise group, the values of cardiac VEGF and HIF-1α were significantly higher than those of the exercise + genistein group(P<0.05).
Conclusion: It seems that intense intermittent exercise has angiogenic effects, but genstein with the dose of the present study leads to the modulation of angiogenesis caused by exercise.
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